Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   REIFOR_RS01625 Genome accession   NZ_CP011797
Coordinates   348160..349194 (+) Length   344 a.a.
NCBI ID   WP_100255902.1    Uniprot ID   A0A2K8KKJ7
Organism   Reinekea forsetii strain Hel1_31_D35     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 343160..354194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  REIFOR_RS01595 (REIFOR_00320) - 343526..343966 (-) 441 WP_100255897.1 DUF4426 domain-containing protein -
  REIFOR_RS01600 (REIFOR_00321) metW 343968..344567 (-) 600 WP_100255898.1 methionine biosynthesis protein MetW -
  REIFOR_RS01605 (REIFOR_00322) - 344557..345705 (-) 1149 WP_100255899.1 homoserine O-acetyltransferase -
  REIFOR_RS01610 (REIFOR_00323) - 345766..346350 (-) 585 WP_100255900.1 YggT family protein -
  REIFOR_RS01615 (REIFOR_00324) proC 346501..347322 (-) 822 WP_100255901.1 pyrroline-5-carboxylate reductase -
  REIFOR_RS01620 (REIFOR_00325) - 347346..348032 (-) 687 WP_227003735.1 YggS family pyridoxal phosphate-dependent enzyme -
  REIFOR_RS01625 (REIFOR_00326) pilT 348160..349194 (+) 1035 WP_100255902.1 type IV pilus twitching motility protein PilT Machinery gene
  REIFOR_RS01630 (REIFOR_00327) pilU 349291..350418 (+) 1128 WP_100255903.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  REIFOR_RS01635 (REIFOR_00328) - 350497..350685 (+) 189 WP_100255904.1 DUF1289 domain-containing protein -
  REIFOR_RS01640 (REIFOR_00329) - 350666..350929 (-) 264 WP_100255905.1 hypothetical protein -
  REIFOR_RS01645 (REIFOR_00330) - 351059..352654 (+) 1596 WP_100255906.1 GMC family oxidoreductase -
  REIFOR_RS01650 (REIFOR_00331) - 352710..353534 (-) 825 WP_100255907.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38117.72 Da        Isoelectric Point: 6.9313

>NTDB_id=148735 REIFOR_RS01625 WP_100255902.1 348160..349194(+) (pilT) [Reinekea forsetii strain Hel1_31_D35]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPPMGHKEVHGLIYDIMNDRQRKAFEEFFETDFSFEVPGVAR
FRVNAFNHNRGSGAVFRTIPSKVLTMEDLGMGQVFKDIADTARGICLVTGPTGSGKSTTLAAMVNYINETKYEHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSSALRSALREDPDIILVGEMRDLETIRLALEAAETGHMVFGTLHTQSAAKTIDRI
VDVFPGAEKSMVRSMLSESLMAVVSQTLLKKTGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGAQHGMQTLDQCL
QNLVQRGTVHRDIARSKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=148735 REIFOR_RS01625 WP_100255902.1 348160..349194(+) (pilT) [Reinekea forsetii strain Hel1_31_D35]
ATGGACATAACCGAATTACTGGCCTTCAGCGCAAAGCAAAATGCCTCCGATCTGCATCTGTCTGCAGGTCTACCGCCGAT
GATCCGGGTCGATGGCGACGTACGTCGAATCAACCTACCGCCCATGGGTCATAAGGAAGTTCATGGCCTAATCTATGACA
TCATGAACGATCGCCAACGGAAGGCTTTCGAAGAGTTTTTCGAAACCGACTTTTCCTTTGAAGTGCCGGGAGTGGCGCGA
TTTCGGGTTAATGCCTTCAATCACAACCGGGGTTCCGGTGCCGTCTTTCGGACCATCCCCTCCAAGGTGCTGACCATGGA
AGACCTCGGTATGGGCCAGGTGTTTAAAGACATCGCCGATACCGCGCGCGGTATCTGTCTGGTCACCGGCCCAACCGGCT
CGGGTAAGTCGACGACCTTGGCGGCGATGGTCAACTACATCAATGAAACCAAGTACGAACATATCCTGACCATCGAAGAC
CCGATCGAATTTGTCCATGAGTCGAAAAAATGCCTGGTGAACCAGCGCGAAGTCCATCGTGACACGCTGGGCTTCAGTTC
GGCCCTGCGCTCAGCCTTGCGTGAAGATCCGGATATCATTCTGGTCGGCGAAATGCGCGATCTGGAAACCATTCGCTTGG
CATTGGAAGCCGCCGAGACCGGCCATATGGTGTTTGGCACCCTACACACCCAATCGGCCGCCAAGACGATTGACCGCATT
GTCGATGTATTTCCCGGCGCGGAAAAGTCCATGGTGCGCTCGATGCTGTCCGAATCCCTAATGGCGGTGGTTTCACAAAC
TCTGCTCAAGAAGACCGGGGGCGGCCGTGTGGCGGCGCACGAAATTATGATTGGCACGCCGGCCATTCGTAACCTGATCC
GCGAAGACAAGGTCGCCCAGATGTACTCCGCGATCCAAACCGGCGCACAACATGGCATGCAAACGCTCGATCAGTGCCTG
CAGAACCTGGTCCAACGCGGCACCGTGCATCGTGACATAGCGCGCTCGAAAGCCAAGCAACCGGAAAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K8KKJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

85.465

100

0.855
  pilT Pseudomonas aeruginosa PAK

84.884

100

0.849
  pilT Acinetobacter nosocomialis M2

80.814

100

0.808
  pilT Acinetobacter baumannii D1279779

80.814

100

0.808
  pilT Acinetobacter baumannii strain A118

80.814

100

0.808
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

73.373

98.256

0.721
  pilT Legionella pneumophila strain ERS1305867

73.373

98.256

0.721
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Pseudomonas stutzeri DSM 10701

40.909

95.93

0.392
  pilU Acinetobacter baylyi ADP1

37.861

100

0.381
  pilU Vibrio cholerae strain A1552

39.692

94.477

0.375

Multiple sequence alignment