Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TVD_RS12705 Genome accession   NZ_CP011367
Coordinates   2734503..2735537 (-) Length   344 a.a.
NCBI ID   WP_047251749.1    Uniprot ID   A0A0G3G743
Organism   Thioalkalivibrio versutus strain D301     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2729503..2740537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TVD_RS12685 (TVD_12685) - 2729868..2731160 (+) 1293 WP_047251745.1 dihydroorotase -
  TVD_RS12690 (TVD_12690) - 2731157..2732065 (+) 909 WP_047251746.1 dihydroorotate dehydrogenase electron transfer subunit -
  TVD_RS12695 (TVD_12695) - 2732131..2733249 (-) 1119 WP_047251747.1 PilT/PilU family type 4a pilus ATPase -
  TVD_RS12700 (TVD_12700) pilU 2733270..2734448 (-) 1179 WP_047251748.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TVD_RS12705 (TVD_12705) pilT 2734503..2735537 (-) 1035 WP_047251749.1 type IV pilus twitching motility protein PilT Machinery gene
  TVD_RS12710 (TVD_12710) - 2735679..2736395 (+) 717 WP_047251750.1 YggS family pyridoxal phosphate-dependent enzyme -
  TVD_RS12715 (TVD_12715) proC 2736392..2737210 (+) 819 WP_047251751.1 pyrroline-5-carboxylate reductase -
  TVD_RS12720 (TVD_12720) - 2737542..2738450 (-) 909 WP_047251752.1 fructosamine kinase family protein -
  TVD_RS12725 (TVD_12725) - 2738520..2738903 (+) 384 WP_047251753.1 group III truncated hemoglobin -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38076.44 Da        Isoelectric Point: 6.1863

>NTDB_id=145170 TVD_RS12705 WP_047251749.1 2734503..2735537(-) (pilT) [Thioalkalivibrio versutus strain D301]
MDITQLLAFSVKNGASDLHISAGMPPMVRIDGDMRRVNVPVMDHKEVQGLIYDIMNDRQRKDYEEFLETDFSFEISGLAR
FRVNAFNQDRGAAAVFRTIPSKVLTLEELGAPRIFEEISDYPRGIVLVTGPTGSGKSTTLAGMVNHRNESDYGHILTIED
PIEFVHESKKSLINQREVHRDTHGFSEALRSALREDPDTILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRAMLSESLRAVIAQTLMKKIGGGRVAAHEIMLGTPAIRNLIRENKIAQMYSTIQTSSGSGMQTLDQCL
KDMLAQGVISREDARRKASNPDSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=145170 TVD_RS12705 WP_047251749.1 2734503..2735537(-) (pilT) [Thioalkalivibrio versutus strain D301]
ATGGATATCACGCAGTTGCTGGCCTTTTCGGTCAAGAACGGGGCCTCGGACCTGCACATCTCCGCGGGCATGCCGCCGAT
GGTGCGTATCGACGGCGACATGCGTCGGGTGAACGTGCCAGTCATGGACCACAAGGAGGTCCAGGGCCTGATCTACGACA
TCATGAACGACCGCCAGCGCAAGGACTACGAGGAGTTCCTGGAGACCGACTTTTCGTTCGAGATCTCGGGGCTCGCGCGC
TTTCGTGTCAACGCCTTCAACCAGGACCGGGGCGCGGCAGCGGTCTTCCGGACCATCCCCTCCAAGGTCCTGACGCTGGA
AGAACTCGGCGCCCCGCGCATCTTCGAGGAGATTTCGGACTACCCGCGCGGCATCGTCCTGGTGACCGGCCCGACCGGTT
CAGGCAAGTCCACCACGCTGGCGGGCATGGTCAACCACCGCAATGAAAGTGACTACGGGCATATCCTCACCATCGAGGAC
CCGATCGAGTTCGTGCACGAATCGAAGAAGTCCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCATGGCTTCAGCGA
GGCCCTGCGTTCGGCCCTGCGCGAGGACCCGGACACCATTCTGGTCGGTGAGCTGCGCGACCTTGAAACCATCCGCCTGG
CCCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCCGCAGCCAAGACCATCGACCGCATC
GTCGACGTGTTCCCGGCCGCGGAGAAGGATATGGTCCGCGCGATGCTGTCGGAGTCGCTGCGTGCGGTCATCGCGCAGAC
CCTGATGAAGAAGATTGGCGGCGGGCGCGTCGCGGCGCACGAGATCATGCTCGGCACGCCGGCGATCCGCAATCTGATCC
GCGAGAACAAGATCGCGCAGATGTACTCGACCATTCAGACCAGTTCCGGCTCGGGCATGCAGACGCTGGACCAGTGCCTG
AAGGACATGCTCGCGCAGGGCGTGATCAGCCGCGAGGACGCACGGCGCAAGGCCAGCAACCCGGATTCCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G3G743

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

77.616

100

0.776
  pilT Pseudomonas aeruginosa PAK

77.616

100

0.776
  pilT Acinetobacter baumannii D1279779

77.616

100

0.776
  pilT Pseudomonas stutzeri DSM 10701

77.326

100

0.773
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

76.453

100

0.765
  pilT Legionella pneumophila strain Lp02

72.059

98.837

0.712
  pilT Legionella pneumophila strain ERS1305867

72.059

98.837

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.03

97.965

0.686
  pilT Vibrio cholerae strain A1552

70.03

97.965

0.686
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.708

99.419

0.494
  pilU Acinetobacter baylyi ADP1

40.751

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

39.71

100

0.398
  pilU Vibrio cholerae strain A1552

39.42

100

0.395

Multiple sequence alignment