Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   YQ44_RS22890 Genome accession   NZ_CP011319
Coordinates   5296631..5297674 (+) Length   347 a.a.
NCBI ID   WP_071325353.1    Uniprot ID   A0A1I9Y2C6
Organism   Janthinobacterium sp. 1_2014MBL_MicDiv     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5291631..5302674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  YQ44_RS22850 (YQ44_22875) - 5291805..5292158 (+) 354 WP_071325345.1 DUF883 family protein -
  YQ44_RS22855 (YQ44_22880) - 5292238..5292624 (+) 387 WP_071325346.1 phage holin family protein -
  YQ44_RS22860 (YQ44_22885) - 5292621..5292971 (+) 351 WP_071325347.1 hypothetical protein -
  YQ44_RS22865 (YQ44_22890) - 5293154..5293627 (+) 474 WP_232251323.1 DUF3455 domain-containing protein -
  YQ44_RS22870 (YQ44_22895) - 5293720..5294286 (+) 567 WP_071325349.1 sigma-70 family RNA polymerase sigma factor -
  YQ44_RS22875 (YQ44_22900) - 5294283..5295002 (+) 720 WP_071325350.1 anti-sigma factor -
  YQ44_RS22880 (YQ44_22905) proC 5295010..5295840 (-) 831 WP_071325351.1 pyrroline-5-carboxylate reductase -
  YQ44_RS22885 (YQ44_22910) - 5295859..5296560 (-) 702 WP_071325352.1 YggS family pyridoxal phosphate-dependent enzyme -
  YQ44_RS22890 (YQ44_22915) pilT 5296631..5297674 (+) 1044 WP_071325353.1 type IV pilus twitching motility protein PilT Machinery gene
  YQ44_RS22895 (YQ44_22920) pilU 5297678..5298817 (+) 1140 WP_269466315.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  YQ44_RS22900 (YQ44_22925) - 5298819..5299304 (+) 486 WP_071325354.1 glutathione peroxidase -
  YQ44_RS22905 (YQ44_22930) - 5299491..5301602 (+) 2112 WP_071325355.1 EAL domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38098.65 Da        Isoelectric Point: 6.7785

>NTDB_id=144463 YQ44_RS22890 WP_071325353.1 5296631..5297674(+) (pilT) [Janthinobacterium sp. 1_2014MBL_MicDiv]
MDISELLAFSVSNKASDLHLSSGLPPMIRVNGDVRRLNVAPLEHKEVHRMIYDIMNDSQRKAYEEALECDFSFEIPGLAR
FRVNAYNQERGASAVLRTIPSKVLTLEELNAPRIFGELAMRPRGLVLVTGPTGSGKSTTLAAMVNHVNERLNHHILTIED
PIEFVHEPKKCLINQREVGSHTHSFSNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKSIDRI
IDVFPAEEKEMVRAMLSESLQAVISQNLLKTKDGAGRVAAHEIMLATPAVRNLIREAKVAQMYSAIQTGANVGMQTLDQC
LSDLVRRGTISAETARSAAKAPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=144463 YQ44_RS22890 WP_071325353.1 5296631..5297674(+) (pilT) [Janthinobacterium sp. 1_2014MBL_MicDiv]
ATGGACATCTCCGAACTACTCGCTTTCTCCGTCAGCAACAAGGCTTCCGACCTGCACCTGTCTTCCGGCCTGCCGCCGAT
GATACGGGTGAATGGCGACGTGCGCCGCCTGAACGTGGCGCCGCTCGAGCACAAGGAAGTGCACCGCATGATCTACGACA
TCATGAACGACAGCCAGCGCAAGGCGTATGAAGAAGCGCTGGAATGCGATTTCTCGTTCGAGATTCCCGGCCTGGCCCGC
TTCCGCGTGAATGCCTACAACCAGGAACGCGGCGCCTCGGCCGTGCTGCGCACGATTCCGTCGAAGGTGCTGACCCTGGA
AGAACTGAACGCCCCGCGCATCTTCGGCGAACTGGCCATGCGCCCGCGCGGCCTCGTGCTGGTGACGGGCCCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCCATGGTCAACCACGTCAACGAGCGCCTGAACCACCACATCCTCACCATCGAAGAC
CCGATCGAGTTCGTGCACGAACCGAAGAAATGCCTGATCAACCAGCGCGAAGTGGGCTCGCACACGCATTCGTTCAGCAA
CGCGCTGCGCTCGGCCCTGCGCGAAGACCCGGACGTCATCCTCGTGGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCCGAGACGGGCCACCTCGTGTTCGGCACCCTGCACACCTCGTCGGCGGCGAAATCGATCGACCGCATC
ATCGACGTCTTCCCCGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAATCGCTGCAAGCCGTCATCTCGCAAAA
CTTGCTGAAAACCAAGGATGGCGCGGGCCGCGTGGCCGCGCATGAAATCATGCTGGCCACGCCGGCCGTGCGCAACCTGA
TCCGCGAAGCGAAAGTGGCGCAAATGTATTCGGCCATCCAGACGGGCGCCAACGTGGGCATGCAAACCCTGGACCAGTGC
CTCTCCGACCTGGTGCGGCGCGGCACGATCTCGGCGGAAACGGCCCGCTCGGCCGCCAAGGCCCCCGAAAACTTCCCCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1I9Y2C6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

74.203

99.424

0.738
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

71.386

97.695

0.697
  pilT Legionella pneumophila strain ERS1305867

71.386

97.695

0.697
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.92

97.118

0.689
  pilT Vibrio cholerae strain A1552

70.92

97.118

0.689
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

99.424

0.524
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Vibrio cholerae strain A1552

40.58

99.424

0.403

Multiple sequence alignment