Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VZ52_RS05220 Genome accession   NZ_CP011257
Coordinates   1112885..1113928 (-) Length   347 a.a.
NCBI ID   WP_045787652.1    Uniprot ID   A0A0D5ASY1
Organism   Ralstonia mannitolilytica strain SN83A39     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1107885..1118928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VZ52_RS05190 (VZ52_05200) - 1108364..1108933 (-) 570 WP_063391851.1 PilX N-terminal domain-containing pilus assembly protein -
  VZ52_RS05195 (VZ52_05205) - 1108937..1109977 (-) 1041 WP_063391852.1 PilW family protein -
  VZ52_RS05200 (VZ52_05210) pilV 1109974..1110588 (-) 615 WP_063391853.1 type IV pilus modification protein PilV -
  VZ52_RS05205 (VZ52_05215) - 1110585..1111058 (-) 474 WP_063391854.1 GspH/FimT family pseudopilin -
  VZ52_RS05210 (VZ52_05220) - 1111063..1111500 (-) 438 WP_063391855.1 type IV pilin protein -
  VZ52_RS05215 (VZ52_05225) pilU 1111692..1112831 (-) 1140 WP_063391856.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VZ52_RS05220 (VZ52_05230) pilT 1112885..1113928 (-) 1044 WP_045787652.1 type IV pilus twitching motility protein PilT Machinery gene
  VZ52_RS05225 (VZ52_05235) - 1114052..1114768 (+) 717 WP_063391857.1 YggS family pyridoxal phosphate-dependent enzyme -
  VZ52_RS05230 (VZ52_05240) proC 1114799..1115623 (+) 825 WP_063391858.1 pyrroline-5-carboxylate reductase -
  VZ52_RS05235 (VZ52_05245) - 1115656..1115931 (+) 276 WP_063391859.1 hypothetical protein -
  VZ52_RS05240 (VZ52_05250) ubiA 1115928..1116803 (-) 876 WP_063393605.1 4-hydroxybenzoate octaprenyltransferase -
  VZ52_RS05245 (VZ52_05255) - 1116868..1118199 (-) 1332 Protein_1054 peroxidase family protein -
  VZ52_RS05250 (VZ52_05260) - 1118261..1118761 (-) 501 WP_045787657.1 Dps family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38264.01 Da        Isoelectric Point: 6.7828

>NTDB_id=143993 VZ52_RS05220 WP_045787652.1 1112885..1113928(-) (pilT) [Ralstonia mannitolilytica strain SN83A39]
MDIAQLLAFSAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHAMVYDIMSDVQRKVYEENLEVDFSFEIPGLSR
FRVNAFNQNRGASAVFRTIPSKVLSLDDLKAPPVFAELAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNENDMGHILTVED
PIEFVHESKKSLINQRELGPHTLSFANALKSALREDPDVILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPSEEKDMVRTMLSESLEAVISQTLLKTRDGSGRIAAHEIMITTPAIRHLIRENKISQMYSMMQTSSGMGMQTLDQC
LADLIKRSAINYADARAIAKNPDAFAN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=143993 VZ52_RS05220 WP_045787652.1 1112885..1113928(-) (pilT) [Ralstonia mannitolilytica strain SN83A39]
ATGGACATCGCGCAGCTTCTGGCCTTCTCGGCCAAGAACAAGGCGTCGGACTTGCATTTGTCGGCCGGCCTGCCGCCGAT
GATCCGGATCCACGGCGACATGCGCCGGATCAACGTGCCGCCGCTCACGCACCAGGATGTGCACGCCATGGTGTACGACA
TCATGAGCGACGTGCAGCGCAAGGTCTACGAAGAAAACCTGGAAGTCGACTTCTCCTTTGAGATCCCGGGCCTGTCGCGC
TTCCGGGTCAACGCGTTCAACCAGAACCGCGGCGCCTCGGCCGTGTTCCGGACGATTCCGTCGAAGGTGCTCTCGCTGGA
CGACCTGAAGGCGCCGCCCGTGTTTGCCGAGCTGGCGATGAAGCCGCGCGGCCTGGTGCTCGTGACCGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCCGCGATGGTCAACCACCGCAACGAAAACGACATGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGAGCCTGATCAACCAGCGCGAACTCGGGCCGCACACGCTGTCGTTCGCCAA
TGCACTGAAGTCGGCGCTGCGTGAAGACCCGGACGTCATCCTCGTCGGCGAATTGCGTGACCTGGAAACGATCCGCCTGG
CGCTGACGGCCGCTGAAACCGGCCACTTGGTGTTTGCCACGCTGCACACGAGTTCTGCGGCCAAGACGATCGACCGGGTG
GTCGACGTGTTCCCGTCGGAAGAGAAGGACATGGTGCGGACCATGCTTTCCGAATCGCTGGAGGCGGTGATCTCGCAGAC
GCTGCTCAAGACGCGCGACGGCTCGGGGCGGATTGCGGCGCACGAGATCATGATCACGACGCCGGCCATCCGACACCTGA
TCCGCGAGAACAAGATCTCGCAGATGTACTCGATGATGCAGACCAGCAGCGGCATGGGCATGCAGACGCTCGACCAGTGC
CTTGCCGACCTCATCAAGCGCTCGGCGATCAACTACGCGGATGCGCGCGCCATCGCCAAGAACCCCGACGCGTTCGCGAA
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D5ASY1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

71.014

99.424

0.706
  pilT Acinetobacter nosocomialis M2

71.014

99.424

0.706
  pilT Acinetobacter baumannii D1279779

71.014

99.424

0.706
  pilT Acinetobacter baumannii strain A118

71.014

99.424

0.706
  pilT Pseudomonas stutzeri DSM 10701

70.725

99.424

0.703
  pilT Acinetobacter baylyi ADP1

69.565

99.424

0.692
  pilT Neisseria gonorrhoeae MS11

68.497

99.712

0.683
  pilT Neisseria meningitidis 8013

68.497

99.712

0.683
  pilT Legionella pneumophila strain Lp02

67.826

99.424

0.674
  pilT Legionella pneumophila strain ERS1305867

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.875

97.118

0.64
  pilT Vibrio cholerae strain A1552

65.875

97.118

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.289

99.712

0.501
  pilU Acinetobacter baylyi ADP1

43.895

99.135

0.435
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403

Multiple sequence alignment