Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VZ52_RS05215 Genome accession   NZ_CP011257
Coordinates   1111692..1112831 (-) Length   379 a.a.
NCBI ID   WP_063391856.1    Uniprot ID   -
Organism   Ralstonia mannitolilytica strain SN83A39     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1106692..1117831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VZ52_RS05190 (VZ52_05200) - 1108364..1108933 (-) 570 WP_063391851.1 PilX N-terminal domain-containing pilus assembly protein -
  VZ52_RS05195 (VZ52_05205) - 1108937..1109977 (-) 1041 WP_063391852.1 PilW family protein -
  VZ52_RS05200 (VZ52_05210) pilV 1109974..1110588 (-) 615 WP_063391853.1 type IV pilus modification protein PilV -
  VZ52_RS05205 (VZ52_05215) - 1110585..1111058 (-) 474 WP_063391854.1 GspH/FimT family pseudopilin -
  VZ52_RS05210 (VZ52_05220) - 1111063..1111500 (-) 438 WP_063391855.1 type IV pilin protein -
  VZ52_RS05215 (VZ52_05225) pilU 1111692..1112831 (-) 1140 WP_063391856.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VZ52_RS05220 (VZ52_05230) pilT 1112885..1113928 (-) 1044 WP_045787652.1 type IV pilus twitching motility protein PilT Machinery gene
  VZ52_RS05225 (VZ52_05235) - 1114052..1114768 (+) 717 WP_063391857.1 YggS family pyridoxal phosphate-dependent enzyme -
  VZ52_RS05230 (VZ52_05240) proC 1114799..1115623 (+) 825 WP_063391858.1 pyrroline-5-carboxylate reductase -
  VZ52_RS05235 (VZ52_05245) - 1115656..1115931 (+) 276 WP_063391859.1 hypothetical protein -
  VZ52_RS05240 (VZ52_05250) ubiA 1115928..1116803 (-) 876 WP_063393605.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42213.59 Da        Isoelectric Point: 6.7253

>NTDB_id=143992 VZ52_RS05215 WP_063391856.1 1111692..1112831(-) (pilU) [Ralstonia mannitolilytica strain SN83A39]
MLDREAATKYIHELLQLMVNSRGSDLFITSDFPPAIKVDGKVTPISQQPLSATQAMGLVKAIMNEKQLREYEESLECNFA
ITAPGAGRFRVSAFVQQGRAGMVLRTINTKIPSLDELDLPPILNEIVMSKRGLVIVVGATGSGKSTSLAAMVGHRNANSY
GHIITIEDPVEYVHAHQNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIINFFPEEKRQQLLIDLSLNLRAMIAQRLLPRKGRKGRVPAVEIMLATPLVQDLIFKGEVHELKEVMKKSREQ
GMISFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHSKRAGQTDLAAGTEHLNVV

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=143992 VZ52_RS05215 WP_063391856.1 1111692..1112831(-) (pilU) [Ralstonia mannitolilytica strain SN83A39]
ATGCTGGACCGCGAAGCCGCCACCAAATACATCCACGAACTCCTGCAGTTGATGGTGAACAGCCGGGGCTCGGACTTGTT
CATCACGTCGGATTTCCCGCCCGCCATCAAGGTGGACGGCAAGGTCACGCCGATCTCGCAGCAGCCGCTGAGCGCCACGC
AGGCCATGGGCCTGGTCAAGGCGATCATGAACGAGAAGCAGCTGCGCGAGTACGAAGAGAGCCTCGAGTGCAACTTCGCC
ATCACGGCGCCGGGGGCCGGGCGCTTCCGCGTGTCGGCCTTCGTGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACGAT
CAACACGAAGATCCCGTCGCTGGATGAGCTCGACCTGCCGCCGATCCTCAACGAGATCGTGATGAGCAAGCGCGGCCTGG
TGATCGTCGTGGGCGCGACCGGCTCGGGCAAGTCGACCTCGCTGGCGGCCATGGTGGGGCATCGCAACGCCAATTCGTAC
GGCCACATCATCACGATCGAAGACCCGGTCGAATACGTGCACGCGCACCAGAACTGCGTGGTCACGCAGCGCGAGGTGGG
CGTCGATACCGAGTCGTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCC
GCGATCGCGACACCATGGAATACGCCATCCAGTATGCGGAAACCGGCCACCTGTGCCTCGCGACGCTGCACGCCAACAGC
TCGAACCAGGCCATCGACCGCATCATCAACTTCTTCCCGGAAGAGAAGCGCCAGCAGTTGCTGATCGACCTCTCGCTGAA
CCTGCGCGCGATGATTGCGCAGCGCCTGCTGCCACGCAAAGGCAGGAAGGGGCGCGTGCCGGCAGTGGAGATCATGCTGG
CCACGCCGCTGGTGCAGGACCTCATCTTCAAGGGCGAGGTGCACGAGCTGAAGGAAGTCATGAAGAAATCGCGCGAGCAG
GGCATGATTTCCTTCGACCAGGCGCTGTTCGAGCTGTACGAGGCGGACAAGATCACCTACGAAGACGCCCTGCGCAACGC
CGACTCGCTCAACGACCTGCGCCTGCAGATCAAGCTGCACAGCAAGCGCGCCGGGCAGACCGACCTGGCGGCCGGCACCG
AGCACCTCAACGTTGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.582

93.404

0.575
  pilU Acinetobacter baylyi ADP1

56

92.348

0.517
  pilU Vibrio cholerae strain A1552

53.736

91.821

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.323

88.918

0.385
  pilT Vibrio cholerae strain A1552

45.426

83.641

0.38
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.426

83.641

0.38
  pilT Legionella pneumophila strain ERS1305867

43.243

87.863

0.38
  pilT Legionella pneumophila strain Lp02

43.243

87.863

0.38
  pilT Acinetobacter baylyi ADP1

44.41

84.96

0.377
  pilT Pseudomonas stutzeri DSM 10701

42.136

88.918

0.375
  pilT Pseudomonas aeruginosa PAK

42.136

88.918

0.375
  pilT Acinetobacter baumannii strain A118

44.201

84.169

0.372
  pilT Acinetobacter nosocomialis M2

44.201

84.169

0.372
  pilT Acinetobacter baumannii D1279779

44.201

84.169

0.372

Multiple sequence alignment