Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WQ53_RS02315 Genome accession   NZ_CP011144
Coordinates   530012..531049 (-) Length   345 a.a.
NCBI ID   WP_052630028.1    Uniprot ID   A0A0E3UM63
Organism   Pseudoxanthomonas suwonensis strain J1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 525012..536049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WQ53_RS02285 (WQ53_02295) - 525089..526033 (-) 945 WP_052630017.1 aspartate carbamoyltransferase catalytic subunit -
  WQ53_RS02290 (WQ53_02300) ruvX 526177..526623 (-) 447 WP_082113102.1 Holliday junction resolvase RuvX -
  WQ53_RS02295 (WQ53_02305) - 526646..527215 (-) 570 WP_052630022.1 YqgE/AlgH family protein -
  WQ53_RS02300 (WQ53_02310) - 527284..528000 (-) 717 WP_330217147.1 YitT family protein -
  WQ53_RS02305 (WQ53_02315) - 528147..528728 (-) 582 WP_052630024.1 hypothetical protein -
  WQ53_RS02310 (WQ53_02320) pilU 528831..529961 (-) 1131 WP_052630026.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WQ53_RS02315 (WQ53_02325) pilT 530012..531049 (-) 1038 WP_052630028.1 type IV pilus twitching motility protein PilT Machinery gene
  WQ53_RS02320 (WQ53_02330) - 531164..531844 (+) 681 WP_052630030.1 YggS family pyridoxal phosphate-dependent enzyme -
  WQ53_RS02325 (WQ53_02335) proC 531891..532730 (+) 840 WP_052630032.1 pyrroline-5-carboxylate reductase -
  WQ53_RS02330 (WQ53_02340) - 532727..533194 (+) 468 WP_052630034.1 DUF4426 domain-containing protein -
  WQ53_RS02335 (WQ53_02345) - 533409..533825 (+) 417 WP_052630036.1 hypothetical protein -
  WQ53_RS02340 (WQ53_02350) - 533878..534738 (-) 861 WP_052633873.1 pirin family protein -
  WQ53_RS02345 (WQ53_02355) - 534870..535529 (-) 660 WP_052630038.1 DUF2306 domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38624.33 Da        Isoelectric Point: 6.6395

>NTDB_id=142693 WQ53_RS02315 WP_052630028.1 530012..531049(-) (pilT) [Pseudoxanthomonas suwonensis strain J1]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSDVLTLEDLGCPPIFRELINQPQGLILVTGPTGSGKSTTLAAMIDHINKDEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRGLVTRHQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=142693 WQ53_RS02315 WP_052630028.1 530012..531049(-) (pilT) [Pseudoxanthomonas suwonensis strain J1]
ATGGATATCGCCGAACTGCTGGCCTTCTCGGTCAAGAACAAGGCTTCCGACCTCCACCTGTCGGCCGGCCTGCCGCCGAT
GATCCGGGTCGACGGCGACGTCCGCCGGATCAACATCCCGGCCCTGGACCACAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTACGAGGAGTTCCTCGAGGTCGACTTCTCGTTCGAGATCCCCTCGCTGGCCCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCAGTGTTCCGCACCATCCCCTCCGACGTGCTGACCCTGGA
GGACCTGGGCTGCCCGCCGATCTTCCGCGAACTGATCAACCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGGACGAGTACGGGCACATCCTCACCGTCGAGGAC
CCGATCGAGTTCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCAACGA
GGCCCTGCGCTCGGCGCTGCGCGAGGACCCGGACTACATCCTGGTCGGCGAAATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCCAGCGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTCCGCTCGATGCTGTCCGAGTCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGCACCGCGGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGTGGCCTGGTCACCCGCCACCAGGCCAAGGAATACGCCAAGGACAAGCGTCTGTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E3UM63

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

78.779

99.71

0.786
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

78.466

98.261

0.771
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

95.362

0.681
  pilT Vibrio cholerae strain A1552

71.429

95.362

0.681
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.205

99.13

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.504
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Pseudomonas stutzeri DSM 10701

40.118

98.261

0.394
  pilU Vibrio cholerae strain A1552

40.923

94.203

0.386

Multiple sequence alignment