Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WQ53_RS02310 Genome accession   NZ_CP011144
Coordinates   528831..529961 (-) Length   376 a.a.
NCBI ID   WP_052630026.1    Uniprot ID   A0A0E3YZ32
Organism   Pseudoxanthomonas suwonensis strain J1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 523831..534961
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WQ53_RS02285 (WQ53_02295) - 525089..526033 (-) 945 WP_052630017.1 aspartate carbamoyltransferase catalytic subunit -
  WQ53_RS02290 (WQ53_02300) ruvX 526177..526623 (-) 447 WP_082113102.1 Holliday junction resolvase RuvX -
  WQ53_RS02295 (WQ53_02305) - 526646..527215 (-) 570 WP_052630022.1 YqgE/AlgH family protein -
  WQ53_RS02300 (WQ53_02310) - 527284..528000 (-) 717 WP_330217147.1 YitT family protein -
  WQ53_RS02305 (WQ53_02315) - 528147..528728 (-) 582 WP_052630024.1 hypothetical protein -
  WQ53_RS02310 (WQ53_02320) pilU 528831..529961 (-) 1131 WP_052630026.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WQ53_RS02315 (WQ53_02325) pilT 530012..531049 (-) 1038 WP_052630028.1 type IV pilus twitching motility protein PilT Machinery gene
  WQ53_RS02320 (WQ53_02330) - 531164..531844 (+) 681 WP_052630030.1 YggS family pyridoxal phosphate-dependent enzyme -
  WQ53_RS02325 (WQ53_02335) proC 531891..532730 (+) 840 WP_052630032.1 pyrroline-5-carboxylate reductase -
  WQ53_RS02330 (WQ53_02340) - 532727..533194 (+) 468 WP_052630034.1 DUF4426 domain-containing protein -
  WQ53_RS02335 (WQ53_02345) - 533409..533825 (+) 417 WP_052630036.1 hypothetical protein -
  WQ53_RS02340 (WQ53_02350) - 533878..534738 (-) 861 WP_052633873.1 pirin family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41874.22 Da        Isoelectric Point: 6.8287

>NTDB_id=142692 WQ53_RS02310 WP_052630026.1 528831..529961(-) (pilU) [Pseudoxanthomonas suwonensis strain J1]
MSSIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETKIPTVEELNLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDTWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRGQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLVQDYIRDGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=142692 WQ53_RS02310 WP_052630026.1 528831..529961(-) (pilU) [Pseudoxanthomonas suwonensis strain J1]
ATGAGCAGCATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCGCGGGCAT
GCCGCCGTCGATGAAGGTCCACGGCAAGATCAGCCCGATCACGCAGACCCCGCTCACGCCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACGCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCGATCGGCGTGGCAGGC
GTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTCCTGCGCCGGATCGAGACCAAGATCCC
GACGGTGGAGGAGCTGAACCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAC
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTCCGCACCCGCGAGGGCA
TGGACCACGCCGTCGCCTTCGCCGAGACCGGCCACCTGGTCCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGGCGCGGCCAGCTGTTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGT
CGCCCAGCAGCTGATACCGACCCCGGACGGCAAGGGCCGCCGGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTCC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGTCCCAGGGCGGCGACGCACGCACCCTGGCGCAGGGGCTGGACGGCGTCGAGATCGCAG
AAGTGCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E3YZ32

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.665

97.606

0.67
  pilU Acinetobacter baylyi ADP1

64.706

94.947

0.614
  pilU Vibrio cholerae strain A1552

53.933

94.681

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

94.947

0.407
  pilT Acinetobacter baumannii strain A118

40.988

91.489

0.375
  pilT Acinetobacter nosocomialis M2

40.988

91.489

0.375
  pilT Acinetobacter baumannii D1279779

40.988

91.489

0.375
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment