Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LA76x_RS07145 Genome accession   NZ_CP011129
Coordinates   1744800..1745837 (+) Length   345 a.a.
NCBI ID   WP_031370959.1    Uniprot ID   A0A0S2F856
Organism   Lysobacter antibioticus strain 76     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1739800..1750837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA76x_RS07120 (LA76x_1539) - 1740429..1741373 (+) 945 WP_057917228.1 triacylglycerol lipase -
  LA76x_RS07125 (LA76x_1540) - 1741473..1742375 (+) 903 WP_187308492.1 lipase secretion chaperone -
  LA76x_RS07130 (LA76x_1541) - 1742560..1743033 (-) 474 WP_057917230.1 DUF4426 domain-containing protein -
  LA76x_RS07135 (LA76x_1542) proC 1743030..1743866 (-) 837 WP_057917231.1 pyrroline-5-carboxylate reductase -
  LA76x_RS07140 (LA76x_1543) - 1743941..1744639 (-) 699 WP_237051751.1 YggS family pyridoxal phosphate-dependent enzyme -
  LA76x_RS07145 (LA76x_1544) pilT 1744800..1745837 (+) 1038 WP_031370959.1 type IV pilus twitching motility protein PilT Machinery gene
  LA76x_RS07150 (LA76x_1545) pilU 1745942..1747120 (+) 1179 WP_051884544.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38522.33 Da        Isoelectric Point: 6.7807

>NTDB_id=142597 LA76x_RS07145 WP_031370959.1 1744800..1745837(+) (pilT) [Lysobacter antibioticus strain 76]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDFEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLSLEDLGCPPIFRQLIEQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYAHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQSHGMMTLDQHL
QDLVKRGMILRPQAREYAKDKRLFD

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=142597 LA76x_RS07145 WP_031370959.1 1744800..1745837(+) (pilT) [Lysobacter antibioticus strain 76]
ATGGATATCGCCGAACTTCTGGCGTTTTCGGTTAAGAACAAAGCATCCGATCTTCATCTGTCGGCAGGTCTGCCGCCGAT
GATCCGGGTCGACGGCGACGTACGCCGCATCAACATTCCGGCCCTGGACCACAAACAGGTCCATGCGCTCGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTTCGAAGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCCGGCCTGGCGCGT
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCGGAAGTGCTGTCGCTGGA
AGACCTCGGCTGCCCGCCGATCTTCCGCCAACTGATCGAACAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTCGCGGCGATGATCGACCACATCAACAAGAACGAGTACGCGCACATCCTGTCGGTCGAAGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTCCATCGCGACACCCACGGCTTCAACGA
GGCCCTGCGTTCGGCGCTGCGCGAAGACCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACTTGGTGTTCGGCACCCTGCATACCTCGTCGGCGGCCAAGACCGTTGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAAAAGCCGATGGTGCGTTCGATGCTGTCGGAGTCCTTGCGCGCGGTGATCTCGCAGGC
CTTGCTCAAGAAGGTCGGCGGCGGTCGTACCGCGGCATGGGAGATCATGGTCGGCATCCCCGCCATCCGTAACCTGATCC
GCGAAGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGAGCCACGGCATGATGACGCTCGACCAGCACCTG
CAAGACCTCGTCAAGCGCGGCATGATCCTGCGGCCGCAGGCGCGCGAGTACGCGAAGGACAAGCGTTTGTTCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0S2F856

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

78.171

98.261

0.768
  pilT Legionella pneumophila strain Lp02

73.837

99.71

0.736
  pilT Legionella pneumophila strain ERS1305867

73.837

99.71

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.909

95.362

0.667
  pilT Vibrio cholerae strain A1552

69.909

95.362

0.667
  pilT Neisseria meningitidis 8013

66.276

98.841

0.655
  pilT Neisseria gonorrhoeae MS11

65.982

98.841

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394
  pilU Vibrio cholerae strain A1552

40.308

94.203

0.38

Multiple sequence alignment