Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LA76x_RS07150 Genome accession   NZ_CP011129
Coordinates   1745942..1747120 (+) Length   392 a.a.
NCBI ID   WP_051884544.1    Uniprot ID   A0A0S2F834
Organism   Lysobacter antibioticus strain 76     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1740942..1752120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA76x_RS07125 (LA76x_1540) - 1741473..1742375 (+) 903 WP_187308492.1 lipase secretion chaperone -
  LA76x_RS07130 (LA76x_1541) - 1742560..1743033 (-) 474 WP_057917230.1 DUF4426 domain-containing protein -
  LA76x_RS07135 (LA76x_1542) proC 1743030..1743866 (-) 837 WP_057917231.1 pyrroline-5-carboxylate reductase -
  LA76x_RS07140 (LA76x_1543) - 1743941..1744639 (-) 699 WP_237051751.1 YggS family pyridoxal phosphate-dependent enzyme -
  LA76x_RS07145 (LA76x_1544) pilT 1744800..1745837 (+) 1038 WP_031370959.1 type IV pilus twitching motility protein PilT Machinery gene
  LA76x_RS07150 (LA76x_1545) pilU 1745942..1747120 (+) 1179 WP_051884544.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 392 a.a.        Molecular weight: 43471.87 Da        Isoelectric Point: 6.6615

>NTDB_id=142598 LA76x_RS07150 WP_051884544.1 1745942..1747120(+) (pilU) [Lysobacter antibioticus strain 76]
MNNPANPNPAGPASPATGAIDFTSFLKLMAHQRASDLFITSGMPPSMKVHGKISPITQNPLTPQQSRDLVLNVMSPQQRE
EFEKTHECNFAIGVTGVGRFRVSCFYQRNQVGMVLRRIETKIPTVEELNLPPIIKTLAMTKRGIIIFVGATGTGKSTSLA
AMIGYRNQNSTGHIITIEDPIEFVHRHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETG
HLVLCTLHANNANQAMDRIINFFPEDRRDQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLAQDYIRDGEIHK
LKELMKESVQLGMKTFDQALFELYQAGEIGYEDALRYADSQNEVRLRIKLAQGGDARTLSQGMDGVEVAEVR

Nucleotide


Download         Length: 1179 bp        

>NTDB_id=142598 LA76x_RS07150 WP_051884544.1 1745942..1747120(+) (pilU) [Lysobacter antibioticus strain 76]
ATGAACAACCCAGCCAATCCCAATCCGGCCGGCCCGGCCAGCCCCGCCACCGGCGCCATCGACTTCACCTCCTTCCTCAA
GCTGATGGCGCACCAGCGCGCCTCGGACTTGTTCATCACCTCGGGCATGCCGCCGTCGATGAAGGTCCACGGCAAGATCA
GCCCGATCACCCAGAACCCGCTGACGCCGCAGCAGAGCCGCGATCTCGTGCTTAACGTGATGAGCCCGCAGCAGCGTGAA
GAGTTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGACCGGCGTCGGCCGCTTCCGCGTGAGCTGCTTTTACCA
GCGCAACCAGGTCGGCATGGTCCTGCGCCGCATCGAGACCAAGATCCCGACCGTCGAAGAACTGAACCTGCCGCCGATCA
TCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCGCCACCGGTACCGGTAAGTCGACCTCGCTCGCG
GCGATGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAATTCGTCCACCGCCA
CGAAGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAGTTGGGAGAACGCGCTCAAGAACACCCTGCGCC
AGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCATGGACCACGCTATCGCCTTCGCCGAAACCGGC
CACTTGGTGTTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCCGAGGACCG
CCGCGACCAGTTGCTGATGGACTTGTCGCTGAACCTCAAGGGCGTGGTCGCCCAGCAGCTGATCCCGACCCCGGACGGCA
AGGGCCGCCGCGTCGCCATGGAAATCCTGCTCGGCACCCCGCTGGCCCAGGACTACATCCGCGACGGCGAGATCCACAAG
CTCAAGGAGCTGATGAAGGAATCCGTGCAGCTCGGCATGAAGACCTTCGACCAGGCCCTGTTCGAGCTCTACCAGGCCGG
CGAGATCGGTTACGAAGACGCCCTGCGTTACGCCGACTCGCAGAACGAAGTGCGCCTGCGCATCAAACTCGCCCAGGGCG
GCGACGCGCGCACCTTGTCGCAAGGCATGGACGGCGTCGAAGTGGCCGAAGTGCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(134-288)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0S2F834

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.665

93.622

0.643
  pilU Acinetobacter baylyi ADP1

64.146

91.071

0.584
  pilU Vibrio cholerae strain A1552

53.371

90.816

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.88

89.541

0.375

Multiple sequence alignment