Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AzCIB_RS19470 Genome accession   NZ_CP011072
Coordinates   4352060..4353100 (+) Length   346 a.a.
NCBI ID   WP_050417415.1    Uniprot ID   -
Organism   Azoarcus sp. CIB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4347060..4358100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AzCIB_RS19440 (AzCIB_3892) - 4347273..4348247 (+) 975 WP_050417410.1 aspartate carbamoyltransferase catalytic subunit -
  AzCIB_RS19445 (AzCIB_3893) - 4348247..4349527 (+) 1281 WP_050417411.1 dihydroorotase -
  AzCIB_RS19450 (AzCIB_3894) - 4349552..4349854 (-) 303 WP_050417412.1 DUF167 family protein -
  AzCIB_RS19455 (AzCIB_3895) - 4349851..4350429 (-) 579 WP_050417413.1 YggT family protein -
  AzCIB_RS19460 (AzCIB_3896) proC 4350435..4351247 (-) 813 WP_050418467.1 pyrroline-5-carboxylate reductase -
  AzCIB_RS19465 (AzCIB_3897) - 4351256..4351957 (-) 702 WP_050417414.1 YggS family pyridoxal phosphate-dependent enzyme -
  AzCIB_RS19470 (AzCIB_3898) pilT 4352060..4353100 (+) 1041 WP_050417415.1 type IV pilus twitching motility protein PilT Machinery gene
  AzCIB_RS19475 (AzCIB_3899) pilU 4353232..4354365 (+) 1134 WP_050417416.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AzCIB_RS19480 (AzCIB_3900) - 4354448..4355608 (-) 1161 WP_050417417.1 THUMP domain-containing protein -
  AzCIB_RS19485 (AzCIB_3901) - 4355694..4356113 (-) 420 WP_050417418.1 CopD family protein -
  AzCIB_RS19490 (AzCIB_3902) - 4356123..4357007 (-) 885 WP_050417419.1 NAD-dependent epimerase/dehydratase family protein -
  AzCIB_RS19495 (AzCIB_3903) - 4357029..4358048 (+) 1020 WP_050417420.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38519.22 Da        Isoelectric Point: 7.0157

>NTDB_id=142034 AzCIB_RS19470 WP_050417415.1 4352060..4353100(+) (pilT) [Azoarcus sp. CIB]
MDITELLAFAVKNKASDLHLSSGLPPMIRVHGDVRRINLPPMEHKDVHSMVYDIMNDQQRKQYEEILECDFSFAVPNLAR
FRVNAFNQNRGAAAVFRTIPSKVLSLEELNCPKIFKDISNQPRGIVLVTGPTGSGKSTTLAAMVDHVNDNEYGHILTIED
PIEFVHESKRCLINQREVARDTLSFNNALRSALREDPDVILVGELRDLETIRLALTGAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGTPAIRNLIRENKIAQMYSSIQTGQNVGMQTLDQC
LLDLVRRNIISPAEAKLKAQNKDNFG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=142034 AzCIB_RS19470 WP_050417415.1 4352060..4353100(+) (pilT) [Azoarcus sp. CIB]
ATGGACATCACCGAACTGCTGGCCTTTGCGGTCAAGAACAAGGCCTCCGACCTCCATCTCTCGTCCGGCCTGCCGCCCAT
GATCCGCGTGCATGGCGACGTGCGGCGCATCAACTTGCCGCCGATGGAGCACAAGGACGTGCACTCCATGGTGTATGACA
TCATGAACGACCAGCAGCGCAAACAGTACGAAGAAATACTGGAATGCGACTTCTCGTTCGCGGTGCCCAACCTCGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCGGCCGCCGTGTTCCGGACCATTCCGTCGAAGGTGCTGAGCCTCGA
AGAGCTCAACTGCCCGAAGATCTTCAAGGACATCTCCAACCAGCCGCGCGGCATCGTGCTCGTCACCGGCCCGACGGGCT
CGGGCAAGTCGACGACCCTCGCGGCGATGGTCGACCACGTCAACGACAACGAATACGGCCACATCCTCACGATCGAGGAC
CCGATCGAATTCGTGCATGAGTCGAAGCGCTGCCTGATCAACCAGCGCGAGGTCGCGCGCGACACGCTGTCGTTCAACAA
CGCGCTGCGTTCGGCGCTGCGCGAAGACCCGGACGTGATCCTCGTCGGCGAATTGCGCGACCTCGAAACCATCCGCCTCG
CGCTGACCGGCGCCGAGACCGGCCACCTCGTATTCGGAACCCTGCACACTTCCTCAGCTGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCGGCCGCCGAAAAGGAGATGGTGCGATCGATGCTGTCCGAATCGCTGCGCGCAGTGATCTCGCAGAC
CCTGCTGAAGACCAAGGACGGCCAGGGCCGCGTCGCCGCGCACGAAATCATGATCGGCACCCCCGCCATCCGCAACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGTCGATCCAGACCGGCCAGAACGTCGGCATGCAGACGCTGGACCAGTGC
CTGCTCGATCTCGTACGCCGCAACATCATCTCGCCCGCGGAAGCCAAGCTCAAGGCGCAGAACAAGGACAACTTCGGCTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.971

99.711

0.777
  pilT Acinetobacter baumannii D1279779

77.971

99.711

0.777
  pilT Acinetobacter baumannii strain A118

77.971

99.711

0.777
  pilT Pseudomonas stutzeri DSM 10701

77.101

99.711

0.769
  pilT Pseudomonas aeruginosa PAK

76.812

99.711

0.766
  pilT Acinetobacter baylyi ADP1

75.942

99.711

0.757
  pilT Legionella pneumophila strain Lp02

73.913

99.711

0.737
  pilT Legionella pneumophila strain ERS1305867

73.913

99.711

0.737
  pilT Neisseria meningitidis 8013

71.884

99.711

0.717
  pilT Neisseria gonorrhoeae MS11

71.594

99.711

0.714
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.941

98.266

0.668
  pilT Vibrio cholerae strain A1552

67.941

98.266

0.668
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.079

95.665

0.517
  pilU Acinetobacter baylyi ADP1

41.954

100

0.422
  pilU Vibrio cholerae strain A1552

39.665

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

40.58

99.711

0.405

Multiple sequence alignment