Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AzCIB_RS19475 Genome accession   NZ_CP011072
Coordinates   4353232..4354365 (+) Length   377 a.a.
NCBI ID   WP_050417416.1    Uniprot ID   -
Organism   Azoarcus sp. CIB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4348232..4359365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AzCIB_RS19445 (AzCIB_3893) - 4348247..4349527 (+) 1281 WP_050417411.1 dihydroorotase -
  AzCIB_RS19450 (AzCIB_3894) - 4349552..4349854 (-) 303 WP_050417412.1 DUF167 family protein -
  AzCIB_RS19455 (AzCIB_3895) - 4349851..4350429 (-) 579 WP_050417413.1 YggT family protein -
  AzCIB_RS19460 (AzCIB_3896) proC 4350435..4351247 (-) 813 WP_050418467.1 pyrroline-5-carboxylate reductase -
  AzCIB_RS19465 (AzCIB_3897) - 4351256..4351957 (-) 702 WP_050417414.1 YggS family pyridoxal phosphate-dependent enzyme -
  AzCIB_RS19470 (AzCIB_3898) pilT 4352060..4353100 (+) 1041 WP_050417415.1 type IV pilus twitching motility protein PilT Machinery gene
  AzCIB_RS19475 (AzCIB_3899) pilU 4353232..4354365 (+) 1134 WP_050417416.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AzCIB_RS19480 (AzCIB_3900) - 4354448..4355608 (-) 1161 WP_050417417.1 THUMP domain-containing protein -
  AzCIB_RS19485 (AzCIB_3901) - 4355694..4356113 (-) 420 WP_050417418.1 CopD family protein -
  AzCIB_RS19490 (AzCIB_3902) - 4356123..4357007 (-) 885 WP_050417419.1 NAD-dependent epimerase/dehydratase family protein -
  AzCIB_RS19495 (AzCIB_3903) - 4357029..4358048 (+) 1020 WP_050417420.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  AzCIB_RS19500 (AzCIB_3904) - 4358053..4359189 (+) 1137 WP_050417421.1 HDOD domain-containing protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42204.55 Da        Isoelectric Point: 6.4203

>NTDB_id=142035 AzCIB_RS19475 WP_050417416.1 4353232..4354365(+) (pilU) [Azoarcus sp. CIB]
MERDQALKFMHDLLRLMVQKNGSDLFITGGFPPAIKIDGRVIPQSNQSLTQQHTAELARAVMNDRQAAEFESTKECNFAI
SPAGIGRFRANAFIQQGKVGLVLRTIAQKIPTFDDLGMPTVLRDVAMAKRGLVIFVGGTGTGKTTSLAAMVDFRNEHSYG
HIITVEDPIEYVHLHKNCIVTQREIGIDTDNWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQALDRIINFFPEDRRQQLLMDLSLNLRALISQRLLPKKGEKGRVPAVEVLLNSPLISDLIFKGEVPGIKEVMKRSRELG
MQTFDQALFDLYEDERITYEDALRNADSVNDLRLQIKLNSKLGEKDLVSGIQHLDIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=142035 AzCIB_RS19475 WP_050417416.1 4353232..4354365(+) (pilU) [Azoarcus sp. CIB]
ATGGAACGCGATCAGGCCCTCAAGTTCATGCACGACCTGCTCAGGCTGATGGTGCAGAAGAACGGCTCCGACCTCTTCAT
CACCGGGGGCTTCCCGCCCGCGATCAAGATCGACGGACGCGTCATCCCGCAATCGAACCAGTCGCTCACGCAGCAGCACA
CCGCCGAACTCGCGCGCGCGGTCATGAACGACCGTCAGGCGGCCGAATTCGAGTCCACCAAGGAATGCAACTTCGCGATC
TCGCCGGCGGGCATCGGCCGCTTCCGTGCCAACGCCTTCATCCAGCAGGGCAAGGTCGGCCTCGTGCTGCGCACGATCGC
GCAGAAGATTCCGACCTTCGACGACTTGGGCATGCCCACGGTGCTGCGCGACGTCGCGATGGCCAAGCGCGGCCTCGTGA
TCTTCGTCGGCGGCACCGGCACCGGCAAGACGACCTCGCTCGCGGCGATGGTGGACTTCCGCAACGAACACTCCTACGGC
CACATCATCACGGTCGAGGACCCGATCGAATACGTGCACCTGCACAAGAATTGCATCGTTACGCAGCGCGAGATCGGCAT
CGACACCGACAACTGGGAAGCGGCACTGAAGAACACCCTGCGCCAAGCCCCGGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGACTACGCGATCGCGTTCGCCGAGACCGGCCACCTGTGCCTCGCCACGCTGCACGCGAACAGCGCA
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCCAGCAGCTGCTGATGGACCTTTCACTCAACCT
GCGGGCGCTGATCTCGCAGCGCCTGCTCCCCAAGAAGGGCGAGAAAGGGCGAGTGCCGGCCGTCGAGGTGCTGCTCAACT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTGCCGGGCATCAAGGAAGTGATGAAGCGCTCGCGCGAACTCGGG
ATGCAGACCTTCGACCAGGCACTCTTCGACCTCTACGAGGACGAGAGGATCACCTACGAGGACGCCCTGCGCAACGCCGA
CTCGGTGAACGACCTGCGCCTGCAGATCAAGCTCAACAGCAAGCTTGGCGAGAAGGACCTCGTGTCAGGAATCCAGCACC
TCGACATCGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(99-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.363

96.552

0.602
  pilU Acinetobacter baylyi ADP1

59.829

93.103

0.557
  pilU Vibrio cholerae strain A1552

56.012

90.451

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.284

88.859

0.385
  pilT Pseudomonas stutzeri DSM 10701

41.493

88.859

0.369
  pilT Pseudomonas aeruginosa PAK

41.194

88.859

0.366
  pilT Legionella pneumophila strain ERS1305867

41.088

87.798

0.361
  pilT Legionella pneumophila strain Lp02

41.088

87.798

0.361

Multiple sequence alignment