Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KO116_RS19680 Genome accession   NZ_CP011052
Coordinates   4305339..4306382 (-) Length   347 a.a.
NCBI ID   WP_035562773.1    Uniprot ID   A0A0D5M885
Organism   Halomonas sp. KO116     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4300339..4311382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KO116_RS19665 (KO116_03974) tyrS 4301636..4302835 (-) 1200 WP_035562778.1 tyrosine--tRNA ligase -
  KO116_RS19670 (KO116_03975) - 4302981..4304120 (+) 1140 WP_035562776.1 anhydro-N-acetylmuramic acid kinase -
  KO116_RS19675 (KO116_03976) pilU 4304171..4305331 (-) 1161 WP_045812190.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KO116_RS19680 (KO116_03977) pilT 4305339..4306382 (-) 1044 WP_035562773.1 type IV pilus twitching motility protein PilT Machinery gene
  KO116_RS19685 (KO116_03978) - 4306415..4307131 (+) 717 WP_035562771.1 YggS family pyridoxal phosphate-dependent enzyme -
  KO116_RS19690 (KO116_03979) proC 4307186..4308010 (+) 825 WP_035562768.1 pyrroline-5-carboxylate reductase -
  KO116_RS19695 (KO116_03980) - 4308080..4308673 (+) 594 WP_035562766.1 YggT family protein -
  KO116_RS19700 (KO116_03981) - 4308740..4309936 (+) 1197 WP_035562763.1 homoserine O-acetyltransferase -
  KO116_RS19705 (KO116_03982) metW 4309936..4310529 (+) 594 WP_035562807.1 methionine biosynthesis protein MetW -
  KO116_RS19710 (KO116_03983) - 4310569..4311018 (+) 450 WP_035562760.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38248.73 Da        Isoelectric Point: 6.1859

>NTDB_id=141842 KO116_RS19680 WP_035562773.1 4305339..4306382(-) (pilT) [Halomonas sp. KO116]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAIDNNDVRKLIYDIMNDRQRRDYEEHLEIDFSFEVPGVAR
FRVNAFTQARGAGAVFRTIPSQVLSMQTLGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRYEHILTIED
PVEFVHASKRCLINQREVHRDTHSFADALRSALREDPDVILVGELRDLETIRLALTAAETGHLVLGTLHTTSAAKTVDRI
IDVFPGEEKAMVRSMLSESLQAVVSQTLLKREGGGRVAAHEILIATSAVRNLIREDKVAQMYSAIQTGGSLGMQTLDSAL
SRLVKEGSVSLQEAQLRSKGTLSIKEE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=141842 KO116_RS19680 WP_035562773.1 4305339..4306382(-) (pilT) [Halomonas sp. KO116]
ATGGATATTACCGAACTGCTAGCATTCTCGGCAAAGCAGAATGCCTCTGACTTGCACCTTTCGGCCGGTTTGCCGCCCAT
GATACGTGTTGATGGCGACATTCGTCGGCTCAATGTGCCTGCTATCGATAATAACGATGTGCGCAAATTGATCTACGACA
TCATGAATGATCGACAGCGGCGCGACTACGAAGAGCATCTGGAAATTGATTTTTCCTTTGAGGTGCCGGGGGTGGCGCGC
TTCCGCGTGAATGCCTTCACCCAAGCGCGCGGTGCAGGGGCGGTGTTTCGCACCATCCCCAGTCAAGTGCTCTCCATGCA
GACCTTGGGGCTAGGCGAGGTGTTTGAGCGCTTAGCGATGTTGCCGCGGGGCTTAGTGCTGGTGACGGGGCCAACGGGCT
CGGGCAAGAGCACCACGCTGGCCGCGATGATCGATTACATTAACGATCATCGTTATGAGCATATTCTCACTATTGAAGAC
CCCGTCGAGTTTGTCCACGCCAGCAAGCGCTGTTTGATTAATCAGCGCGAAGTGCACCGCGACACCCATAGCTTTGCCGA
TGCGCTGCGCAGCGCGCTGCGTGAAGACCCGGATGTGATTCTAGTTGGTGAGCTGCGTGATCTTGAAACTATCCGCTTGG
CGTTGACCGCTGCGGAAACAGGGCATTTGGTACTGGGGACGCTACACACCACTTCGGCAGCTAAAACCGTTGATCGGATT
ATCGATGTCTTTCCCGGTGAAGAGAAAGCCATGGTGCGCTCCATGCTCTCTGAATCACTCCAGGCAGTGGTGTCACAAAC
CCTGCTTAAACGTGAGGGCGGTGGGCGCGTGGCGGCCCACGAAATCCTTATTGCCACGTCTGCAGTGCGCAACTTGATTC
GCGAAGACAAAGTGGCGCAGATGTACTCGGCCATTCAAACCGGTGGCAGCTTGGGCATGCAAACGCTGGATTCCGCGCTG
TCCCGGTTAGTGAAAGAGGGTTCGGTAAGTTTGCAAGAAGCGCAGCTGAGGTCAAAGGGAACGCTGTCGATAAAGGAGGA
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D5M885

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.171

97.695

0.764
  pilT Pseudomonas aeruginosa PAK

78.171

97.695

0.764
  pilT Acinetobacter nosocomialis M2

76.401

97.695

0.746
  pilT Acinetobacter baumannii D1279779

76.401

97.695

0.746
  pilT Acinetobacter baumannii strain A118

76.401

97.695

0.746
  pilT Acinetobacter baylyi ADP1

75.516

97.695

0.738
  pilT Legionella pneumophila strain Lp02

72.239

96.542

0.697
  pilT Legionella pneumophila strain ERS1305867

72.239

96.542

0.697
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.565

99.424

0.692
  pilT Vibrio cholerae strain A1552

69.565

99.424

0.692
  pilT Neisseria meningitidis 8013

65.507

99.424

0.651
  pilT Neisseria gonorrhoeae MS11

65.217

99.424

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

55.215

93.948

0.519
  pilU Pseudomonas stutzeri DSM 10701

41.888

97.695

0.409
  pilU Vibrio cholerae strain A1552

41.493

96.542

0.401
  pilU Acinetobacter baylyi ADP1

40.774

96.83

0.395

Multiple sequence alignment