Detailed information NTG0141841

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KO116_RS19675
• Genome accession: NZ_CP011052
• Coordinates: 4304171..4305331 (-)
• Length: 386 a.a.
• NCBI ID: WP_045812190.1
• Uniprot ID: A0A0D5M7S1
• Organism: Halomonas sp. KO116
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4299171..4310331

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KO116_RS19665 (KO116_03974)	tyrS	4301636..4302835 (-)	1200	WP_035562778.1	tyrosine--tRNA ligase	-
KO116_RS19670 (KO116_03975)	-	4302981..4304120 (+)	1140	WP_035562776.1	anhydro-N-acetylmuramic acid kinase	-
KO116_RS19675 (KO116_03976)	pilU	4304171..4305331 (-)	1161	WP_045812190.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KO116_RS19680 (KO116_03977)	pilT	4305339..4306382 (-)	1044	WP_035562773.1	type IV pilus twitching motility protein PilT	Machinery gene
KO116_RS19685 (KO116_03978)	-	4306415..4307131 (+)	717	WP_035562771.1	YggS family pyridoxal phosphate-dependent enzyme	-
KO116_RS19690 (KO116_03979)	proC	4307186..4308010 (+)	825	WP_035562768.1	pyrroline-5-carboxylate reductase	-
KO116_RS19695 (KO116_03980)	-	4308080..4308673 (+)	594	WP_035562766.1	YggT family protein	-
KO116_RS19700 (KO116_03981)	-	4308740..4309936 (+)	1197	WP_035562763.1	homoserine O-acetyltransferase	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 42696.87 Da
• Isoelectric Point: 6.2243

>NTDB_id=141841 KO116_RS19675 WP_045812190.1 4304171..4305331(-) (pilU) [Halomonas sp. KO116]
MSQDAQLSPAQWLHQLLDIMVEQNASDLLISVGAPPSLKMPDGLVPLGQQRLTAHQVSELVTHALPEGLRERFTCEHEAN
FALSREGKWRFRVSAFQQRNQMAMVVRSIAMEIPCLEALGVPEQLAELAQAKRGLVLVVGGTGTGKSTTLAAMIQQRNET
VGGHIISIEDPIEYLHPHKRAIVNQREVGIDTESFEVALKNTLRQAPDVILIGEIRTRETMEHALTFAETGHLCLATLHA
NNANQALERIIHFFPHERHEQIWMDLSLNLRAVVAQQLLPTLAGGRCAAIEIMLQSPRIADLIRKGEVAEMKAAMAKSRD
AGMQTFDQALHDLHQAGHISREVAMAHADSANDLRMLLNVSDAKGRNLSLDEQVPSHLSLRDGDDY

Nucleotide

• Length: 1161 bp

>NTDB_id=141841 KO116_RS19675 WP_045812190.1 4304171..4305331(-) (pilU) [Halomonas sp. KO116]
ATGAGTCAAGATGCCCAGCTTTCGCCCGCCCAGTGGCTGCACCAGCTGCTTGATATTATGGTTGAGCAAAACGCTTCTGA
TTTGCTCATCTCCGTGGGTGCGCCGCCAAGCTTGAAAATGCCCGATGGGTTGGTGCCCTTGGGTCAGCAACGCTTAACCG
CGCATCAGGTAAGTGAGCTGGTTACTCACGCTCTACCGGAAGGATTGCGTGAACGTTTTACCTGTGAGCATGAAGCGAAT
TTCGCGCTCAGCCGAGAAGGCAAGTGGCGCTTTCGGGTCAGCGCGTTTCAGCAGCGCAATCAGATGGCCATGGTAGTGCG
CAGCATCGCGATGGAAATTCCTTGCCTAGAGGCGCTGGGTGTACCAGAACAGTTGGCAGAATTAGCTCAGGCCAAACGGG
GGCTGGTACTGGTGGTGGGTGGCACGGGAACGGGTAAGTCGACGACCCTGGCGGCGATGATTCAGCAGCGCAACGAAACC
GTTGGCGGCCATATCATCAGCATCGAAGACCCCATTGAGTACTTGCACCCGCACAAGCGCGCTATCGTCAATCAGCGCGA
GGTGGGGATCGATACCGAATCGTTTGAGGTGGCGCTAAAAAATACCCTGCGCCAGGCGCCGGATGTGATTCTGATTGGTG
AAATCCGCACCCGAGAAACCATGGAACACGCACTGACCTTTGCTGAAACCGGCCATCTCTGCCTGGCAACGCTACATGCC
AATAACGCCAATCAGGCGTTAGAGCGCATCATTCACTTCTTCCCTCATGAACGCCATGAGCAGATATGGATGGATCTGTC
TCTGAATCTGCGTGCAGTCGTCGCCCAGCAGCTGCTGCCCACGCTTGCAGGCGGGCGCTGTGCTGCTATCGAAATCATGC
TGCAGTCGCCGCGCATCGCCGACTTGATCCGTAAAGGGGAGGTCGCCGAGATGAAAGCAGCGATGGCCAAGTCCCGCGAT
GCCGGAATGCAGACCTTCGATCAGGCGCTGCATGATCTGCATCAGGCGGGGCATATCAGCAGAGAGGTGGCCATGGCACA
CGCCGACTCGGCTAACGACCTGCGCATGCTGCTCAATGTTAGTGACGCCAAGGGGCGTAACCTCTCGCTAGATGAACAGG
TGCCGAGTCATTTAAGCCTACGCGATGGCGATGATTACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (78-280)

3D structure

• AlphaFold DB: A0A0D5M7S1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.667	97.15	0.57
pilU	Vibrio cholerae strain A1552	57.714	90.674	0.523
pilU	Acinetobacter baylyi ADP1	54.121	94.301	0.51
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.385	94.301	0.381
pilT	Legionella pneumophila strain Lp02	42.727	85.492	0.365
pilT	Legionella pneumophila strain ERS1305867	42.727	85.492	0.365
pilT	Pseudomonas aeruginosa PAK	41.667	87.047	0.363
pilT	Pseudomonas stutzeri DSM 10701	41.369	87.047	0.36