Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSPO_RS10465 Genome accession   NZ_CP011039
Coordinates   2253526..2254584 (-) Length   352 a.a.
NCBI ID   WP_010561719.1    Uniprot ID   A0A0P9JY82
Organism   Pseudoalteromonas spongiae UST010723-006     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2248526..2259584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSPO_RS10435 (PSPO_a2343) rsmE 2248674..2249417 (+) 744 WP_010561713.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PSPO_RS10440 (PSPO_a2344) gshB 2249472..2250425 (+) 954 WP_010561714.1 glutathione synthase -
  PSPO_RS10445 (PSPO_a2346) - 2250455..2251024 (+) 570 WP_010561715.1 YqgE/AlgH family protein -
  PSPO_RS10450 (PSPO_a2347) ruvX 2251025..2251465 (+) 441 WP_010561716.1 Holliday junction resolvase RuvX -
  PSPO_RS10455 (PSPO_a2348) - 2251475..2252371 (-) 897 WP_010561717.1 hypothetical protein -
  PSPO_RS10460 (PSPO_a2350) pilU 2252386..2253516 (-) 1131 WP_010561718.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSPO_RS10465 (PSPO_a2351) pilT 2253526..2254584 (-) 1059 WP_010561719.1 type IV pilus twitching motility protein PilT Machinery gene
  PSPO_RS10470 (PSPO_a2352) - 2254622..2255308 (+) 687 WP_010561720.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSPO_RS10475 (PSPO_a2353) proC 2255324..2256154 (+) 831 Protein_1987 pyrroline-5-carboxylate reductase -
  PSPO_RS10480 (PSPO_a2354) - 2256154..2256690 (+) 537 WP_010561722.1 YggT family protein -
  PSPO_RS10485 (PSPO_a2355) - 2256783..2257223 (+) 441 WP_010561723.1 DUF4426 domain-containing protein -
  PSPO_RS10490 (PSPO_a2356) - 2257578..2259257 (-) 1680 WP_010561724.1 alkaline phosphatase -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38850.10 Da        Isoelectric Point: 6.5018

>NTDB_id=141686 PSPO_RS10465 WP_010561719.1 2253526..2254584(-) (pilT) [Pseudoalteromonas spongiae UST010723-006]
MDITELLAFSVQHNASDLHLSSGVSPMIRVDGDVRRINIPALGPKDVNGLVYDIMNDKQRKEYEENLECDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSTVLTLDDLGCPEIFKDISNNPRGLVLVTGPTGSGKSTTLAAMIDYINSHKHHHVLTIED
PIEFVHDNKQSLINQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMVRSMLSESLRAVISQTLVKKVGGGRIAAHEIMIGTPAIRNLIREDKIAQMYSSLQTGASHGMQTMDQCL
GNLLNRGLITQQDAYAKAHDKSNFGAAGARSY

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=141686 PSPO_RS10465 WP_010561719.1 2253526..2254584(-) (pilT) [Pseudoalteromonas spongiae UST010723-006]
ATGGACATTACTGAATTACTGGCATTCAGTGTGCAACATAATGCATCAGATTTACACTTATCATCAGGTGTTTCACCTAT
GATCCGCGTTGATGGCGACGTTCGAAGAATCAATATCCCCGCACTTGGACCAAAAGACGTAAACGGCTTAGTGTATGATA
TTATGAATGATAAGCAGCGAAAAGAATACGAAGAAAACTTAGAATGTGACTTTTCTTTCGAAGTGCCAAACCTAGCGCGT
TTCCGTGTTAATGCCTTTAATTCTAACCGTGGTCCAGCCGCGGTATTTCGTACCATCCCAAGCACCGTTTTAACCCTTGA
TGACTTAGGCTGTCCTGAAATATTCAAAGATATTTCCAATAACCCACGTGGCTTAGTGTTGGTAACGGGACCGACAGGTT
CGGGTAAATCAACAACGCTTGCCGCGATGATTGATTATATTAATTCGCATAAGCACCACCACGTACTTACCATTGAAGAT
CCTATCGAATTCGTACACGACAATAAGCAAAGCTTAATTAACCAACGTGAAGTACACCGTGATACGCACAGCTTTAATAA
CGCACTTCGCTCGGCGCTTCGTGAAGATCCAGATGTTATTCTGGTCGGTGAATTACGTGATTTAGAGACCATTCGCCTTG
CAATGACCGCGGCAGAAACCGGTCACTTAGTATTTGGCACCTTGCACACCACCTCAGCACCAAAAACCATTGACCGTATT
ATCGACGTATTCCCAGGTGAAGAAAAAGACATGGTACGTTCAATGCTATCTGAATCGTTACGTGCTGTAATTTCACAAAC
ACTCGTGAAAAAAGTAGGTGGCGGACGAATCGCAGCACACGAAATCATGATAGGTACCCCTGCTATTCGTAACCTTATTC
GTGAAGATAAAATTGCGCAAATGTATTCATCGCTACAAACCGGTGCATCGCACGGCATGCAGACGATGGATCAGTGTTTA
GGTAACTTGCTAAATCGCGGCTTAATTACACAGCAAGATGCATATGCAAAAGCCCACGATAAGAGCAACTTTGGCGCAGC
AGGCGCACGATCTTACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0P9JY82

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.744

97.727

0.75
  pilT Acinetobacter baumannii D1279779

76.744

97.727

0.75
  pilT Acinetobacter baumannii strain A118

76.744

97.727

0.75
  pilT Pseudomonas stutzeri DSM 10701

76.744

97.727

0.75
  pilT Acinetobacter baylyi ADP1

76.453

97.727

0.747
  pilT Pseudomonas aeruginosa PAK

75.291

97.727

0.736
  pilT Legionella pneumophila strain Lp02

72.093

97.727

0.705
  pilT Legionella pneumophila strain ERS1305867

72.093

97.727

0.705
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.107

95.739

0.69
  pilT Vibrio cholerae strain A1552

72.107

95.739

0.69
  pilT Neisseria meningitidis 8013

68.116

98.011

0.668
  pilT Neisseria gonorrhoeae MS11

67.826

98.011

0.665
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

98.011

0.52
  pilU Pseudomonas stutzeri DSM 10701

41.57

97.727

0.406
  pilU Vibrio cholerae strain A1552

40.988

97.727

0.401
  pilU Acinetobacter baylyi ADP1

39.884

98.295

0.392

Multiple sequence alignment