Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PSPO_RS10460 Genome accession   NZ_CP011039
Coordinates   2252386..2253516 (-) Length   376 a.a.
NCBI ID   WP_010561718.1    Uniprot ID   -
Organism   Pseudoalteromonas spongiae UST010723-006     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2247386..2258516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSPO_RS10430 (PSPO_a2342) - 2247701..2248600 (-) 900 WP_010561712.1 ABC transporter ATP-binding protein -
  PSPO_RS10435 (PSPO_a2343) rsmE 2248674..2249417 (+) 744 WP_010561713.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PSPO_RS10440 (PSPO_a2344) gshB 2249472..2250425 (+) 954 WP_010561714.1 glutathione synthase -
  PSPO_RS10445 (PSPO_a2346) - 2250455..2251024 (+) 570 WP_010561715.1 YqgE/AlgH family protein -
  PSPO_RS10450 (PSPO_a2347) ruvX 2251025..2251465 (+) 441 WP_010561716.1 Holliday junction resolvase RuvX -
  PSPO_RS10455 (PSPO_a2348) - 2251475..2252371 (-) 897 WP_010561717.1 hypothetical protein -
  PSPO_RS10460 (PSPO_a2350) pilU 2252386..2253516 (-) 1131 WP_010561718.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSPO_RS10465 (PSPO_a2351) pilT 2253526..2254584 (-) 1059 WP_010561719.1 type IV pilus twitching motility protein PilT Machinery gene
  PSPO_RS10470 (PSPO_a2352) - 2254622..2255308 (+) 687 WP_010561720.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSPO_RS10475 (PSPO_a2353) proC 2255324..2256154 (+) 831 Protein_1987 pyrroline-5-carboxylate reductase -
  PSPO_RS10480 (PSPO_a2354) - 2256154..2256690 (+) 537 WP_010561722.1 YggT family protein -
  PSPO_RS10485 (PSPO_a2355) - 2256783..2257223 (+) 441 WP_010561723.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41794.61 Da        Isoelectric Point: 6.3008

>NTDB_id=141685 PSPO_RS10460 WP_010561718.1 2252386..2253516(-) (pilU) [Pseudoalteromonas spongiae UST010723-006]
MHGLDHFLKQMVESEASDLFVSTFLPVSVKVNGELRPIGHSDLTEEEALELVESAMTGKQKTEFHETKECNFAIARDSGR
FRVSAFWQRDKAGMVVRRIVTTIPEVHDLGLPPVLTDVIMSKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIED
PIEFVHEHQKSIVTQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDRI
MHLVPKEKHDKLKYDLALNLRAIVAQQLVPTSDGHGRAAAIEILLNSPMVAELIKRGEVGAIKETMSKSREMGMQTFDQA
LFDLYQQHRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTVDGLDGDKNQ

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=141685 PSPO_RS10460 WP_010561718.1 2252386..2253516(-) (pilU) [Pseudoalteromonas spongiae UST010723-006]
ATGCACGGCTTAGATCATTTTTTAAAACAAATGGTAGAGAGTGAAGCATCGGATTTATTCGTGTCTACATTCTTGCCTGT
AAGTGTAAAAGTTAATGGCGAACTTCGCCCGATTGGCCATTCAGATTTAACTGAAGAAGAAGCGTTAGAGCTCGTTGAAT
CGGCAATGACCGGCAAGCAGAAAACCGAATTTCACGAAACCAAAGAATGTAACTTTGCGATCGCACGTGATTCGGGTCGT
TTCCGTGTTTCGGCTTTTTGGCAGCGCGACAAAGCGGGCATGGTTGTTCGTCGTATCGTAACTACCATTCCGGAAGTACA
CGACCTTGGCTTACCACCGGTTTTAACCGATGTGATAATGTCAAAACGTGGCTTAGTCCTGTTTGTGGGTGGTACGGGTA
CCGGTAAATCAACCTCACTTGCGGCACTGTTAGGGTATCGTAACCGCAACCAGCGCGGTCATATCTTAACTATTGAGGAC
CCGATTGAATTTGTTCATGAACACCAAAAGAGTATTGTAACGCAACGCGAAGTGGGACTGGATACTGAAAGTTTCGAATC
GGCACTAAAGAGCTCTCTACGTCAGGCTCCTGATGTAATTTTGATTGGTGAGATCCGCTCGCAAGAAACTATGGAATATG
CGCTTTCATTTGCTGAAACAGGTCATTTGTGTGTGGCTACACTGCACGCAAACAACGCGAACCAAGCAATCGACCGTATT
ATGCACTTAGTGCCAAAAGAAAAGCACGACAAGTTAAAGTACGATTTAGCACTTAACTTGCGTGCTATCGTTGCACAGCA
ACTTGTGCCGACCAGTGATGGTCATGGTCGTGCAGCAGCAATTGAAATATTGCTTAACTCGCCAATGGTGGCAGAACTAA
TAAAACGTGGTGAAGTAGGGGCAATTAAAGAAACCATGTCTAAATCGCGCGAAATGGGTATGCAAACCTTTGACCAAGCG
CTCTTTGACCTTTATCAGCAGCATCGCATTAATTATGCCGATGCACTACACCATGCTGACTCACCGAACGACCTGCGCTT
AATGATTAAATTACGCAATAATGAGCAACAAGGTGCAGGCTTTTTACAAGGTGTTACGGTTGATGGCCTTGATGGAGACA
AAAACCAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.358

99.468

0.59
  pilU Vibrio cholerae strain A1552

57.534

97.074

0.559
  pilU Acinetobacter baylyi ADP1

55.2

99.734

0.551
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.382

96.011

0.407
  pilT Acinetobacter baylyi ADP1

41.265

88.298

0.364

Multiple sequence alignment