Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PTUN_RS03735 Genome accession   NZ_CP011032
Coordinates   795017..796066 (+) Length   349 a.a.
NCBI ID   WP_009838361.1    Uniprot ID   A4C935
Organism   Pseudoalteromonas tunicata strain D2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 790017..801066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PTUN_RS03710 (PTUN_a0884) - 792129..792578 (-) 450 WP_009838356.1 DUF4426 domain-containing protein -
  PTUN_RS03715 (PTUN_a0885) yggU 792605..792910 (-) 306 WP_009838357.1 DUF167 family protein YggU -
  PTUN_RS03720 (PTUN_a0886) - 792910..793443 (-) 534 WP_009838358.1 YggT family protein -
  PTUN_RS03725 (PTUN_a0887) proC 793453..794271 (-) 819 WP_009838359.1 pyrroline-5-carboxylate reductase -
  PTUN_RS03730 (PTUN_a0888) - 794284..795018 (-) 735 WP_009838360.1 YggS family pyridoxal phosphate-dependent enzyme -
  PTUN_RS03735 (PTUN_a0889) pilT 795017..796066 (+) 1050 WP_009838361.1 type IV pilus twitching motility protein PilT Machinery gene
  PTUN_RS03740 (PTUN_a0890) pilU 796069..797199 (+) 1131 WP_009838362.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PTUN_RS03745 (PTUN_a0891) - 797364..798266 (+) 903 WP_009838363.1 hypothetical protein -
  PTUN_RS03750 (PTUN_a0892) ruvX 798378..798827 (-) 450 WP_009838364.1 Holliday junction resolvase RuvX -
  PTUN_RS03755 (PTUN_a0893) - 798859..799416 (-) 558 WP_009838365.1 YqgE/AlgH family protein -
  PTUN_RS03760 (PTUN_a0894) gshB 799444..800397 (-) 954 WP_009838366.1 glutathione synthase -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38594.11 Da        Isoelectric Point: 6.6369

>NTDB_id=141612 PTUN_RS03735 WP_009838361.1 795017..796066(+) (pilT) [Pseudoalteromonas tunicata strain D2]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPVLEAKDVNGLVYDIMNDKQRKEYEENLECDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSDILSLDDLGAPDIFKTISDNPRGLVLVTGPTGSGKSTTLAAMVDYINRNKHHHILTIED
PIEFVHQNKLCLINQREVHRDTKSFSAALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMVRSMLSESLRAVISQTLLKKISGGRVAAHEIMIGVPAIRNLIREDKIAQMYSTIQTGASHGMQTMDQCL
MNLLNRGIISQSQAQSKAHDKAQFGGTGN

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=141612 PTUN_RS03735 WP_009838361.1 795017..796066(+) (pilT) [Pseudoalteromonas tunicata strain D2]
ATGGATATTACCGAATTACTCGCTTTTAGTGTGCAACATAAGGCGTCCGATTTACATTTATCGTCGGGTGTCTCACCTAT
GATCCGAGTTGATGGTGATGTACGGCGCATAAATATTCCAGTCCTCGAAGCCAAAGACGTCAATGGACTGGTCTATGACA
TTATGAATGATAAACAGCGTAAAGAATACGAAGAAAATCTGGAGTGTGACTTTTCATTCGAAGTCCCCAATCTAGCACGT
TTTCGTGTCAATGCCTTTAATTCTAACCGTGGTCCTGCTGCGGTATTTCGTACCATTCCAAGTGATATATTATCGCTAGA
TGATTTAGGTGCGCCGGATATTTTTAAAACCATTTCAGATAATCCTCGGGGTTTGGTATTAGTGACGGGGCCTACGGGTT
CGGGTAAATCGACCACTTTAGCTGCTATGGTTGATTATATTAATCGTAATAAACATCATCATATTTTAACCATTGAAGAC
CCTATCGAGTTTGTGCATCAAAATAAGTTATGCCTAATTAACCAACGTGAAGTGCATCGCGATACCAAAAGTTTTAGTGC
GGCTCTTCGAAGCGCATTACGTGAAGATCCTGATGTGATTCTAGTCGGTGAGTTACGTGACCTTGAAACCATTCGCCTTG
CCATGACAGCGGCAGAAACGGGTCACTTAGTATTTGGCACCTTGCATACCACCTCAGCGCCTAAAACCATTGACCGTATT
ATCGACGTTTTTCCAGGCGAAGAAAAAGATATGGTAAGGTCGATGTTATCTGAGTCGTTGCGGGCTGTTATTTCCCAAAC
CTTATTGAAGAAAATTAGTGGTGGGCGAGTCGCAGCCCATGAAATCATGATCGGTGTACCGGCCATTCGAAATTTAATTC
GTGAAGATAAGATTGCGCAAATGTATTCGACTATTCAAACCGGTGCGTCGCATGGGATGCAAACTATGGATCAATGTTTA
ATGAATTTGCTTAATCGCGGCATTATCAGTCAATCACAAGCGCAGTCAAAAGCTCACGATAAAGCGCAATTTGGCGGTAC
AGGAAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A4C935

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

98.567

0.762
  pilT Acinetobacter baumannii D1279779

77.326

98.567

0.762
  pilT Acinetobacter baumannii strain A118

77.326

98.567

0.762
  pilT Pseudomonas stutzeri DSM 10701

76.163

98.567

0.751
  pilT Pseudomonas aeruginosa PAK

75.872

98.567

0.748
  pilT Acinetobacter baylyi ADP1

75.872

98.567

0.748
  pilT Legionella pneumophila strain Lp02

74.128

98.567

0.731
  pilT Legionella pneumophila strain ERS1305867

74.128

98.567

0.731
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.513

96.562

0.691
  pilT Vibrio cholerae strain A1552

71.513

96.562

0.691
  pilT Neisseria meningitidis 8013

69.208

97.708

0.676
  pilT Neisseria gonorrhoeae MS11

68.915

97.708

0.673
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.143

100

0.513
  pilU Acinetobacter baylyi ADP1

40.882

97.421

0.398
  pilU Pseudomonas stutzeri DSM 10701

41.194

95.989

0.395
  pilU Vibrio cholerae strain A1552

41.194

95.989

0.395

Multiple sequence alignment