Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PESP_RS02845 Genome accession   NZ_CP011028
Coordinates   636111..637151 (+) Length   346 a.a.
NCBI ID   WP_004586840.1    Uniprot ID   -
Organism   Pseudoalteromonas espejiana DSM 9414     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 631111..642151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PESP_RS02815 (PESP_a0628) hemW 631755..632891 (-) 1137 WP_089346681.1 radical SAM family heme chaperone HemW -
  PESP_RS02820 (PESP_a0629) - 632888..633487 (-) 600 WP_089346682.1 XTP/dITP diphosphatase -
  PESP_RS02825 (PESP_a0630) - 633492..633935 (-) 444 WP_089346683.1 DUF4426 domain-containing protein -
  PESP_RS02830 (PESP_a0631) - 633996..634529 (-) 534 WP_004586837.1 YggT family protein -
  PESP_RS02835 (PESP_a0632) proC 634556..635377 (-) 822 WP_089346684.1 pyrroline-5-carboxylate reductase -
  PESP_RS02840 (PESP_a0633) - 635395..636108 (-) 714 WP_089346685.1 YggS family pyridoxal phosphate-dependent enzyme -
  PESP_RS02845 (PESP_a0634) pilT 636111..637151 (+) 1041 WP_004586840.1 type IV pilus twitching motility protein PilT Machinery gene
  PESP_RS02850 (PESP_a0635) pilU 637161..638294 (+) 1134 WP_089346686.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PESP_RS02855 (PESP_a0636) ruvX 638386..638835 (-) 450 WP_058549466.1 Holliday junction resolvase RuvX -
  PESP_RS02860 (PESP_a0637) - 638845..639402 (-) 558 WP_089346687.1 YqgE/AlgH family protein -
  PESP_RS02865 (PESP_a0638) gshB 639527..640477 (-) 951 WP_089346688.1 glutathione synthase -
  PESP_RS02870 (PESP_a0639) rsmE 640532..641266 (-) 735 WP_089346689.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PESP_RS02875 (PESP_a0640) - 641340..641816 (-) 477 WP_089346690.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38141.37 Da        Isoelectric Point: 6.1844

>NTDB_id=141563 PESP_RS02845 WP_004586840.1 636111..637151(+) (pilT) [Pseudoalteromonas espejiana DSM 9414]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPALADKDVNSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSDVLTLEDLGAPDIFKTISDNPRGLVLVTGPTGSGKSTTLAAMVDYINQTKHHHILTIED
PIEFVHDNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMVRSMLSESLRAVISQTLLKKIGGGRVAAHEIMIGVPAIRNLIREDKIAQMYSSIQTGASHGMQTMDQCL
NNLVNHGIVTTAAAKEKAQDKTQFGG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=141563 PESP_RS02845 WP_004586840.1 636111..637151(+) (pilT) [Pseudoalteromonas espejiana DSM 9414]
ATGGATATTACCGAATTATTAGCGTTTAGTGTGCAACACAAAGCATCCGATTTACACTTATCGTCGGGTGTTTCACCTAT
GATACGCGTAGATGGTGATGTTCGCCGCATTAATATACCTGCACTTGCAGACAAAGACGTAAACAGCCTTGTATACGACA
TTATGAACGATAATCAACGTAAGGACTACGAACAAAACCTAGAAGTGGATTTTTCGTTTGAAGTACCTAACCTTGCGCGT
TTTCGTGTGAATGCATTTAACTCAAACCGTGGCCCTGCGGCAGTATTTCGTACTATCCCAAGCGATGTACTTACGCTGGA
AGATTTAGGTGCCCCCGATATTTTTAAAACAATTTCAGATAACCCGCGTGGATTGGTATTGGTTACAGGCCCAACGGGTT
CGGGTAAGTCAACAACACTTGCGGCTATGGTTGATTACATAAATCAAACAAAACATCACCATATCCTCACTATAGAAGAC
CCAATCGAATTTGTTCACGACAATAAACTAAGCCTGATTAACCAGCGTGAAGTACACCGTGATACGCACAGTTTTTCTAA
TGCACTTCGTAGTGCGCTGCGTGAAGACCCCGATGTAATACTCGTTGGTGAGCTACGTGATTTAGAAACAATTCGCCTTG
CTATGACCGCAGCAGAAACGGGTCACCTAGTATTTGGTACATTGCATACTACATCAGCGCCAAAAACCATTGACCGTATT
ATTGACGTATTCCCAGGTGAAGAAAAAGACATGGTGCGTTCAATGCTTTCTGAGTCGTTACGTGCAGTAATTTCGCAAAC
ACTGCTTAAAAAAATTGGCGGTGGGCGTGTGGCTGCGCACGAAATTATGATTGGTGTTCCAGCTATTCGTAACTTAATTC
GTGAGGATAAAATAGCGCAAATGTATTCGTCTATTCAAACGGGTGCATCGCATGGCATGCAAACTATGGATCAATGTTTA
AATAATTTGGTTAATCACGGCATAGTAACAACCGCCGCTGCCAAAGAAAAAGCCCAAGACAAAACACAGTTTGGTGGCTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.326

99.422

0.769
  pilT Acinetobacter nosocomialis M2

77.035

99.422

0.766
  pilT Acinetobacter baumannii D1279779

77.035

99.422

0.766
  pilT Acinetobacter baumannii strain A118

77.035

99.422

0.766
  pilT Pseudomonas aeruginosa PAK

76.453

99.422

0.76
  pilT Acinetobacter baylyi ADP1

76.453

99.422

0.76
  pilT Legionella pneumophila strain Lp02

72.384

99.422

0.72
  pilT Legionella pneumophila strain ERS1305867

72.384

99.422

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.386

97.977

0.699
  pilT Vibrio cholerae strain A1552

71.386

97.977

0.699
  pilT Neisseria gonorrhoeae MS11

70.674

98.555

0.697
  pilT Neisseria meningitidis 8013

70.674

98.555

0.697
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.711

0.52
  pilU Vibrio cholerae strain A1552

42.462

93.931

0.399
  pilU Acinetobacter baylyi ADP1

38.824

98.266

0.382
  pilU Pseudomonas stutzeri DSM 10701

39.879

95.665

0.382

Multiple sequence alignment