Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PESP_RS02850 Genome accession   NZ_CP011028
Coordinates   637161..638294 (+) Length   377 a.a.
NCBI ID   WP_089346686.1    Uniprot ID   -
Organism   Pseudoalteromonas espejiana DSM 9414     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 632161..643294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PESP_RS02820 (PESP_a0629) - 632888..633487 (-) 600 WP_089346682.1 XTP/dITP diphosphatase -
  PESP_RS02825 (PESP_a0630) - 633492..633935 (-) 444 WP_089346683.1 DUF4426 domain-containing protein -
  PESP_RS02830 (PESP_a0631) - 633996..634529 (-) 534 WP_004586837.1 YggT family protein -
  PESP_RS02835 (PESP_a0632) proC 634556..635377 (-) 822 WP_089346684.1 pyrroline-5-carboxylate reductase -
  PESP_RS02840 (PESP_a0633) - 635395..636108 (-) 714 WP_089346685.1 YggS family pyridoxal phosphate-dependent enzyme -
  PESP_RS02845 (PESP_a0634) pilT 636111..637151 (+) 1041 WP_004586840.1 type IV pilus twitching motility protein PilT Machinery gene
  PESP_RS02850 (PESP_a0635) pilU 637161..638294 (+) 1134 WP_089346686.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PESP_RS02855 (PESP_a0636) ruvX 638386..638835 (-) 450 WP_058549466.1 Holliday junction resolvase RuvX -
  PESP_RS02860 (PESP_a0637) - 638845..639402 (-) 558 WP_089346687.1 YqgE/AlgH family protein -
  PESP_RS02865 (PESP_a0638) gshB 639527..640477 (-) 951 WP_089346688.1 glutathione synthase -
  PESP_RS02870 (PESP_a0639) rsmE 640532..641266 (-) 735 WP_089346689.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PESP_RS02875 (PESP_a0640) - 641340..641816 (-) 477 WP_089346690.1 hypothetical protein -
  PESP_RS02880 (PESP_a0641) - 641898..642791 (-) 894 WP_089346691.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41777.74 Da        Isoelectric Point: 6.1358

>NTDB_id=141564 PESP_RS02850 WP_089346686.1 637161..638294(+) (pilU) [Pseudoalteromonas espejiana DSM 9414]
MNLSLNHFLLIMIEKKGSDLFVSSQLPVSAKINGELIALSDDKLTDEQALQLVESAMSDKQKNEFHTTKECNFAIATDEG
RFRISAFWQRDRAGMVIRRIVTQIPDVSDLGLPSTLTDVIMAKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTDSFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIVAQQLIPTSDGEGRVAAIEILLNSPMVAELIKSGDIGGIKEAMSKSKEMGMQTFDQ
ALFELYKQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKNGN

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=141564 PESP_RS02850 WP_089346686.1 637161..638294(+) (pilU) [Pseudoalteromonas espejiana DSM 9414]
ATGAATTTATCTTTAAACCACTTTTTACTCATTATGATTGAGAAAAAAGGCTCTGACTTATTTGTATCAAGCCAGTTGCC
TGTCAGCGCTAAAATTAATGGCGAACTAATCGCGTTAAGCGACGATAAGCTAACTGACGAACAGGCTTTACAGCTTGTTG
AGTCTGCCATGAGCGACAAGCAAAAAAATGAGTTTCATACCACCAAAGAGTGTAACTTTGCGATAGCCACAGACGAAGGG
CGTTTTCGTATTTCGGCTTTTTGGCAGCGCGACAGAGCAGGCATGGTTATTCGCCGAATAGTAACGCAAATACCTGATGT
AAGCGATTTAGGGCTGCCTTCAACACTTACCGATGTAATTATGGCAAAGCGTGGCTTGGTGTTGTTTGTGGGTGGTACAG
GTACCGGTAAATCAACCTCATTAGCTGCATTAATTGGCTATAGAAACCGGAATCAGCGTGGTCACATTTTAACAATAGAA
GACCCTATTGAGTTTGTTCATGAGCATCGTAAAAGTATTATTACTCAGCGCGAAGTAGGCCTAGATACCGACAGCTTTGA
ATCGGCACTTAAAAGCTCACTACGCCAAGCTCCCGATGTAATATTAATTGGTGAAATACGTTCGCAAGAAACCATGGAAT
ATGCACTGAGCTTTGCTGAAACCGGCCACTTATGTGTAGCCACATTGCATGCTAATAACGCAAACCAAGCCATTGACCGT
ATTATGCATTTAGTGCCTAAAGAAAAGCACGACAAGCTTAAGTACGATTTAGCGCTTAACTTACGCGCTATTGTAGCGCA
GCAGCTTATTCCTACCTCAGATGGGGAAGGGCGTGTTGCCGCCATTGAAATATTATTAAACTCACCCATGGTGGCAGAGC
TAATAAAAAGTGGCGATATTGGCGGCATTAAAGAGGCTATGTCTAAGTCTAAAGAAATGGGCATGCAAACCTTTGACCAA
GCACTGTTTGAGCTATATAAACAGCAACGTATTAATTACGCTGATGCGCTGCATCATGCCGACTCGCCAAACGATTTACG
CTTGATGATTAAACTGCGCAATAACGAGCAGCAAGGCGCAGGCTTTTTACAAGGTGTAACCATAGATGGGTTAGATGATA
AAAACGGTAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.614

100

0.568
  pilU Acinetobacter baylyi ADP1

56.233

100

0.562
  pilU Vibrio cholerae strain A1552

55.586

97.347

0.541
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.105

95.756

0.403

Multiple sequence alignment