Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PAGA_RS02785 Genome accession   NZ_CP011011
Coordinates   621187..622227 (+) Length   346 a.a.
NCBI ID   WP_004586840.1    Uniprot ID   -
Organism   Pseudoalteromonas agarivorans DSM 14585     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 616187..627227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PAGA_RS02755 (PAGA_a0635) hemW 616834..617970 (-) 1137 WP_096041186.1 radical SAM family heme chaperone HemW -
  PAGA_RS02760 (PAGA_a0636) - 617967..618566 (-) 600 WP_055254892.1 XTP/dITP diphosphatase -
  PAGA_RS02765 (PAGA_a0637) - 618571..619014 (-) 444 WP_096041187.1 DUF4426 domain-containing protein -
  PAGA_RS02770 (PAGA_a0638) - 619072..619605 (-) 534 WP_096041188.1 YggT family protein -
  PAGA_RS02775 (PAGA_a0639) proC 619632..620453 (-) 822 WP_096041189.1 pyrroline-5-carboxylate reductase -
  PAGA_RS02780 (PAGA_a0640) - 620471..621184 (-) 714 WP_096041190.1 YggS family pyridoxal phosphate-dependent enzyme -
  PAGA_RS02785 (PAGA_a0641) pilT 621187..622227 (+) 1041 WP_004586840.1 type IV pilus twitching motility protein PilT Machinery gene
  PAGA_RS02790 (PAGA_a0642) pilU 622237..623370 (+) 1134 WP_029772387.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PAGA_RS02795 (PAGA_a0643) ruvX 623504..623953 (-) 450 WP_004586842.1 Holliday junction resolvase RuvX -
  PAGA_RS02800 (PAGA_a0644) - 623963..624520 (-) 558 WP_004586843.1 YqgE/AlgH family protein -
  PAGA_RS02805 (PAGA_a0645) gshB 624645..625595 (-) 951 WP_096041191.1 glutathione synthase -
  PAGA_RS02810 (PAGA_a0646) rsmE 625671..626405 (-) 735 WP_096041192.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PAGA_RS02815 (PAGA_a0647) - 626478..626954 (-) 477 WP_024031544.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38141.37 Da        Isoelectric Point: 6.1844

>NTDB_id=141433 PAGA_RS02785 WP_004586840.1 621187..622227(+) (pilT) [Pseudoalteromonas agarivorans DSM 14585]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPALADKDVNSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSDVLTLEDLGAPDIFKTISDNPRGLVLVTGPTGSGKSTTLAAMVDYINQTKHHHILTIED
PIEFVHDNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMVRSMLSESLRAVISQTLLKKIGGGRVAAHEIMIGVPAIRNLIREDKIAQMYSSIQTGASHGMQTMDQCL
NNLVNHGIVTTAAAKEKAQDKTQFGG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=141433 PAGA_RS02785 WP_004586840.1 621187..622227(+) (pilT) [Pseudoalteromonas agarivorans DSM 14585]
ATGGATATTACCGAATTATTAGCGTTTAGTGTGCAACACAAAGCATCTGATTTACACTTATCGTCAGGTGTTTCACCTAT
GATACGCGTAGATGGTGATGTTCGCCGCATTAATATACCTGCACTTGCAGACAAAGACGTAAACAGCCTTGTTTACGACA
TTATGAACGATAATCAGCGCAAGGACTACGAACAAAATCTAGAAGTGGATTTTTCGTTTGAAGTGCCTAACCTTGCTCGG
TTTCGTGTTAACGCATTTAACTCAAACCGCGGCCCTGCAGCGGTGTTTCGTACCATCCCTAGCGATGTACTGACGCTTGA
AGATTTAGGTGCGCCAGATATTTTTAAAACAATATCAGATAACCCGCGTGGTTTAGTATTGGTTACCGGCCCAACTGGTT
CGGGTAAATCAACAACGCTTGCGGCTATGGTCGATTATATAAATCAAACAAAACATCACCATATCCTCACCATTGAAGAT
CCTATCGAATTTGTACACGATAATAAACTCAGTCTTATTAACCAACGTGAAGTGCACCGAGATACGCACAGCTTCTCTAA
TGCACTGCGCAGCGCGCTGCGTGAAGACCCGGATGTAATACTTGTTGGTGAGCTACGTGACTTAGAAACTATTCGCCTTG
CGATGACCGCCGCTGAAACTGGCCACTTAGTGTTTGGTACGCTACATACCACCTCAGCACCAAAAACGATTGACCGTATT
ATTGATGTATTCCCAGGTGAAGAAAAAGATATGGTGCGCTCAATGCTTTCTGAGTCACTGCGTGCCGTTATTTCGCAAAC
ACTACTTAAAAAAATTGGCGGTGGACGCGTGGCCGCGCACGAAATTATGATTGGTGTACCGGCTATTCGTAACTTAATTC
GTGAAGATAAAATTGCACAAATGTATTCGTCTATTCAAACGGGTGCATCACACGGTATGCAAACCATGGATCAATGTTTA
AATAATTTAGTTAATCACGGTATTGTGACAACCGCAGCCGCAAAAGAAAAAGCCCAAGATAAAACCCAGTTTGGTGGCTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.326

99.422

0.769
  pilT Acinetobacter nosocomialis M2

77.035

99.422

0.766
  pilT Acinetobacter baumannii D1279779

77.035

99.422

0.766
  pilT Acinetobacter baumannii strain A118

77.035

99.422

0.766
  pilT Pseudomonas aeruginosa PAK

76.453

99.422

0.76
  pilT Acinetobacter baylyi ADP1

76.453

99.422

0.76
  pilT Legionella pneumophila strain Lp02

72.384

99.422

0.72
  pilT Legionella pneumophila strain ERS1305867

72.384

99.422

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.386

97.977

0.699
  pilT Vibrio cholerae strain A1552

71.386

97.977

0.699
  pilT Neisseria gonorrhoeae MS11

70.674

98.555

0.697
  pilT Neisseria meningitidis 8013

70.674

98.555

0.697
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.711

0.52
  pilU Vibrio cholerae strain A1552

42.462

93.931

0.399
  pilU Acinetobacter baylyi ADP1

38.824

98.266

0.382
  pilU Pseudomonas stutzeri DSM 10701

39.879

95.665

0.382

Multiple sequence alignment