Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   UC35_RS11910 Genome accession   NZ_CP010951
Coordinates   2461984..2463027 (+) Length   347 a.a.
NCBI ID   WP_061499766.1    Uniprot ID   A0A127JUD5
Organism   Ramlibacter tataouinensis strain 5-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2456984..2468027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UC35_RS11890 (UC35_11905) - 2458605..2459084 (+) 480 WP_061499755.1 winged helix-turn-helix transcriptional regulator -
  UC35_RS11895 (UC35_11910) glpK 2459124..2460617 (+) 1494 WP_061499758.1 glycerol kinase GlpK -
  UC35_RS11900 (UC35_11915) - 2460664..2461203 (+) 540 WP_061499761.1 DUF3617 domain-containing protein -
  UC35_RS11905 (UC35_11920) - 2461218..2461910 (-) 693 WP_061499764.1 YggS family pyridoxal phosphate-dependent enzyme -
  UC35_RS11910 (UC35_11925) pilT 2461984..2463027 (+) 1044 WP_061499766.1 type IV pilus twitching motility protein PilT Machinery gene
  UC35_RS11915 (UC35_11930) pilU 2463039..2464175 (+) 1137 WP_061499769.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  UC35_RS11920 (UC35_11935) - 2464272..2464868 (-) 597 WP_227820318.1 hypothetical protein -
  UC35_RS11925 (UC35_11940) - 2465013..2466077 (+) 1065 WP_415752705.1 NAD(P)-dependent oxidoreductase -
  UC35_RS11930 (UC35_11945) - 2466117..2466887 (-) 771 WP_415752673.1 BON domain-containing protein -
  UC35_RS11935 (UC35_11950) - 2466890..2467489 (-) 600 WP_061499779.1 phosphoheptose isomerase -
  UC35_RS11940 (UC35_11955) - 2467530..2468000 (-) 471 WP_145979408.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38139.91 Da        Isoelectric Point: 6.7708

>NTDB_id=140859 UC35_RS11910 WP_061499766.1 2461984..2463027(+) (pilT) [Ramlibacter tataouinensis strain 5-10]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKAVHAMVYDIMNDTQRKNYEEFLECDFSFEIDGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLNAPKIFGDLALKPRGMVLVTGPTGSGKSTTLAAMINFLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFANALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMIRAMLSESLQAVISQTLCKTKDGSGRVAAHEIMIGTSAIRNLIREAKVAQMYSAIQTGNSFGMQTLDQN
LSDLVKRNVISPAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=140859 UC35_RS11910 WP_061499766.1 2461984..2463027(+) (pilT) [Ramlibacter tataouinensis strain 5-10]
GTGGATATTACCCAGCTGTTGGCATTCAGCGTCAAAAACAAGGCCTCGGACCTGCATCTGTCCGCCGGCCTGCCGCCCAT
GATTCGCGTCCATGGTGACGTGCGGCGTATCAACGTCGATGCGCTGGACCACAAGGCCGTGCACGCCATGGTGTACGACA
TCATGAACGACACCCAGCGCAAGAACTACGAAGAGTTCCTGGAGTGCGACTTCTCGTTCGAGATCGACGGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACGATTCCTTCCAAGATCCTCACGCTGGA
GCAGCTCAACGCGCCCAAGATCTTCGGCGACCTGGCGCTCAAGCCGCGCGGCATGGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACACTGGCCGCGATGATCAACTTCCTGAACGAGACCGAGTACGGCCACATCCTGACGGTCGAGGAC
CCGATCGAGTTCGTGCACGAATCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACGCTGTCCTTCGCCAA
CGCGCTGAAGTCGGCGCTGCGGGAGGACCCGGACGCGATCCTGGTGGGCGAAATGCGCGACCTGGAAACGATCCGGCTGG
CGATGACGGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCTTGCACACCTCGAGCGCCGCCAAGACCATCGACCGAATC
ATCGACGTGTTCCCGGCCGAGGAAAAGGAAATGATCCGGGCCATGCTTTCCGAATCGCTGCAGGCGGTGATCTCACAGAC
GCTGTGCAAGACCAAGGACGGTTCGGGCCGGGTCGCCGCGCACGAGATCATGATCGGCACCTCGGCCATCCGCAACCTCA
TTCGCGAAGCCAAGGTCGCCCAGATGTACTCGGCGATCCAGACCGGCAACAGTTTCGGCATGCAGACGCTCGACCAGAAC
CTCTCCGACCTGGTCAAGCGCAACGTGATCAGCCCCGCCGAAGCGCGCGGCAAGGCCAAGATTCCCGAGAATTTCCCCGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127JUD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.681

99.424

0.772
  pilT Acinetobacter baumannii strain A118

77.681

99.424

0.772
  pilT Acinetobacter nosocomialis M2

77.681

99.424

0.772
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

76.522

99.424

0.761
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Legionella pneumophila strain Lp02

72.566

97.695

0.709
  pilT Legionella pneumophila strain ERS1305867

72.566

97.695

0.709
  pilT Neisseria gonorrhoeae MS11

68.406

99.424

0.68
  pilT Neisseria meningitidis 8013

68.406

99.424

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.569

98.271

0.654
  pilT Vibrio cholerae strain A1552

66.569

98.271

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Acinetobacter baylyi ADP1

42.197

99.712

0.421
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406

Multiple sequence alignment