Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   UC35_RS11915 Genome accession   NZ_CP010951
Coordinates   2463039..2464175 (+) Length   378 a.a.
NCBI ID   WP_061499769.1    Uniprot ID   A0A127JU46
Organism   Ramlibacter tataouinensis strain 5-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2458039..2469175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UC35_RS11890 (UC35_11905) - 2458605..2459084 (+) 480 WP_061499755.1 winged helix-turn-helix transcriptional regulator -
  UC35_RS11895 (UC35_11910) glpK 2459124..2460617 (+) 1494 WP_061499758.1 glycerol kinase GlpK -
  UC35_RS11900 (UC35_11915) - 2460664..2461203 (+) 540 WP_061499761.1 DUF3617 domain-containing protein -
  UC35_RS11905 (UC35_11920) - 2461218..2461910 (-) 693 WP_061499764.1 YggS family pyridoxal phosphate-dependent enzyme -
  UC35_RS11910 (UC35_11925) pilT 2461984..2463027 (+) 1044 WP_061499766.1 type IV pilus twitching motility protein PilT Machinery gene
  UC35_RS11915 (UC35_11930) pilU 2463039..2464175 (+) 1137 WP_061499769.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  UC35_RS11920 (UC35_11935) - 2464272..2464868 (-) 597 WP_227820318.1 hypothetical protein -
  UC35_RS11925 (UC35_11940) - 2465013..2466077 (+) 1065 WP_415752705.1 NAD(P)-dependent oxidoreductase -
  UC35_RS11930 (UC35_11945) - 2466117..2466887 (-) 771 WP_415752673.1 BON domain-containing protein -
  UC35_RS11935 (UC35_11950) - 2466890..2467489 (-) 600 WP_061499779.1 phosphoheptose isomerase -
  UC35_RS11940 (UC35_11955) - 2467530..2468000 (-) 471 WP_145979408.1 hypothetical protein -
  UC35_RS11945 (UC35_11960) - 2468098..2468481 (-) 384 WP_061499784.1 YraN family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41737.11 Da        Isoelectric Point: 6.9721

>NTDB_id=140860 UC35_RS11915 WP_061499769.1 2463039..2464175(+) (pilU) [Ramlibacter tataouinensis strain 5-10]
MERDQASKFINDLLKLMVSRNGSDLFITGDFPPAIKVDGKVTKVSPQPLTGGHTLALARSIMNDKQAAEFERTKECNFAI
SPPGIGRFRVNAFVQQGKVGMVLRVIPQVLPTIDGLGVPQVLKEVVMTKRGLVVLVGATGSGKSTTLAAMVDWRNDNSHG
HIITIEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNALRQAPDVILMGEIRDRETMEHAIAFAETGHLCMATLHANSA
NQALDRVINFFPEERRAQLLMDLSLNLKAMVSQRLVPKQDGKGRGAAVEIMLNTPLVSDLIFKGEVTEIKEIMKKSRNLG
MQTFDQALFDMYENNIISYEDALRNADSLNDLRLQIKLNSQRAKTSDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=140860 UC35_RS11915 WP_061499769.1 2463039..2464175(+) (pilU) [Ramlibacter tataouinensis strain 5-10]
ATGGAACGCGATCAGGCATCGAAATTCATCAACGACCTGCTCAAGCTCATGGTCAGCCGCAATGGCAGCGACCTGTTCAT
CACCGGCGACTTCCCGCCCGCCATCAAGGTCGACGGCAAGGTGACCAAGGTGTCGCCGCAGCCGCTGACCGGCGGGCACA
CGCTGGCGCTGGCGCGCTCGATCATGAACGACAAGCAGGCGGCCGAATTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCGCCCGGCATCGGCCGGTTCCGCGTCAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGGTGATCCC
GCAGGTGCTGCCGACGATCGATGGCCTGGGCGTGCCGCAGGTGCTCAAGGAAGTGGTGATGACCAAGCGCGGCCTGGTCG
TGCTGGTCGGTGCCACCGGCTCGGGCAAGTCGACCACACTGGCCGCGATGGTCGACTGGCGCAATGACAATTCGCACGGC
CACATCATCACCATCGAGGATCCGGTGGAATTCGTGCACCCGCACAAGAACTGCGTGGTGACCCAGCGCGAGGTCGGACT
GGACACCGACAGCTGGGAAGCGGCGCTGAAGAACGCGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCCATCGCCTTCGCCGAAACCGGTCACCTGTGCATGGCGACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCGTGATCAACTTCTTCCCCGAAGAGCGCCGCGCGCAGCTGCTGATGGACCTGTCGCTGAACCT
CAAGGCCATGGTGTCGCAGCGCCTGGTGCCCAAGCAGGACGGCAAGGGCCGCGGCGCCGCGGTGGAGATCATGCTGAACA
CCCCGCTGGTGTCCGACCTGATCTTCAAGGGTGAGGTGACCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACATGTACGAGAACAACATCATTTCCTACGAGGACGCGCTGCGCAACGCCGA
CTCGCTCAACGACCTGCGCCTGCAGATCAAGCTGAACAGCCAGCGCGCCAAGACGTCGGACCTGGCGGCGGGCACCGAGC
ACTTTGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127JU46

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

57.66

94.974

0.548
  pilU Vibrio cholerae strain A1552

53.736

92.063

0.495
  pilT Pseudomonas aeruginosa PAK

45.401

89.153

0.405
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.917

89.153

0.392
  pilT Legionella pneumophila strain ERS1305867

43.844

88.095

0.386
  pilT Legionella pneumophila strain Lp02

43.844

88.095

0.386
  pilT Acinetobacter nosocomialis M2

43.544

88.095

0.384
  pilT Acinetobacter baumannii D1279779

43.544

88.095

0.384
  pilT Acinetobacter baumannii strain A118

43.544

88.095

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.164

83.862

0.37
  pilT Vibrio cholerae strain A1552

44.164

83.862

0.37
  pilT Acinetobacter baylyi ADP1

42.042

88.095

0.37

Multiple sequence alignment