Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TO73_RS00250 Genome accession   NZ_CP010822
Coordinates   50607..51695 (-) Length   362 a.a.
NCBI ID   WP_003046845.1    Uniprot ID   -
Organism   Thermus aquaticus Y51MC23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 45607..56695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TO73_RS00235 (TO73_0054) - 47555..48184 (-) 630 WP_003046837.1 histidine phosphatase family protein -
  TO73_RS00240 (TO73_0055) purM 48181..49182 (-) 1002 WP_003046840.1 phosphoribosylformylglycinamidine cyclo-ligase -
  TO73_RS00245 (TO73_0056) gatB 49188..50597 (-) 1410 WP_003046842.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  TO73_RS00250 (TO73_0057) pilT 50607..51695 (-) 1089 WP_003046845.1 type IV pilus twitching motility protein PilT Machinery gene
  TO73_RS00255 (TO73_0058) pilF 51707..54376 (-) 2670 WP_003046848.1 type IV pilus assembly ATPase PilB Machinery gene
  TO73_RS00260 (TO73_0059) - 54373..54855 (-) 483 WP_003046850.1 YqeG family HAD IIIA-type phosphatase -
  TO73_RS00265 (TO73_0060) pgeF 54880..55614 (-) 735 WP_003046852.1 peptidoglycan editing factor PgeF -
  TO73_RS00270 (TO73_0061) - 55685..56089 (+) 405 WP_003046855.1 DUF4395 domain-containing protein -
  TO73_RS00275 (TO73_0062) ribH 56076..56549 (-) 474 WP_003046858.1 6,7-dimethyl-8-ribityllumazine synthase -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 40065.50 Da        Isoelectric Point: 7.2376

>NTDB_id=140091 TO73_RS00250 WP_003046845.1 50607..51695(-) (pilT) [Thermus aquaticus Y51MC23]
MPKVPDIVDLLSLAVERGASDLMITVGVPPMLKIDGEFHPTEYEPLSPQETRRLMYALMDEKQQRVFEEDKELDFSFSLP
GRGRFRVNVFLQRGSVAGVLRVVPAVIKSFEELGLPKNIAEIALSPRGLVLVTGPTGSGKSTTLASMIDYINERKPVHIV
TIEDPIEFFHKHKKAIVNQREIGADTHGFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIVDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKTHQLRSVIQTGGQFGMIT
MDACLADLYKRKLITYELGLARAVDPKEFMRLAGVQEKPARG

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=140091 TO73_RS00250 WP_003046845.1 50607..51695(-) (pilT) [Thermus aquaticus Y51MC23]
ATGCCTAAGGTTCCGGACATCGTGGATCTTCTGAGCCTGGCCGTGGAGCGAGGGGCCAGCGACCTGATGATCACCGTGGG
CGTCCCCCCCATGCTGAAGATCGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCTCCCCCCAGGAGACCCGCCGCC
TCATGTACGCCCTCATGGACGAAAAGCAGCAGCGGGTCTTTGAGGAGGACAAGGAGCTGGACTTCTCCTTCAGCCTCCCG
GGCAGGGGACGCTTTCGGGTGAACGTCTTCTTGCAGCGGGGAAGCGTGGCCGGGGTGTTGCGGGTGGTGCCCGCCGTCAT
CAAGAGCTTTGAGGAGCTGGGCCTGCCCAAAAACATCGCCGAGATCGCCCTAAGCCCCAGGGGCCTAGTCCTGGTCACGG
GGCCCACGGGGTCAGGGAAGAGCACGACCCTGGCCTCCATGATCGACTACATCAACGAACGCAAACCCGTGCACATCGTG
ACCATTGAGGACCCCATTGAGTTCTTCCACAAGCACAAAAAGGCCATCGTCAACCAGCGGGAGATCGGCGCCGACACCCA
CGGCTTCCACAAGGCCCTAAGGAGCGTCCTCCGCCAGGCCCCCGACGTGATCCTGGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCCGAGACCGGCCACCTGGTCATGGGCACCCTGCACACCAACTCCGCCCCCGAGACC
ATTGACCGCATCGTTGACGTCTTCCCCGAGAACCAGCAGGAGCAGGTGCGGGTCCAGCTCTCCAACAACCTGGTGGCCGT
CCTGACCCAGCAGCTCCTGCCCAAGGCCTTCGGCGGGGGCCGGGTCCTGGCCTACGAGCTCATGATCGCCACCCCGGCCG
TTAGGGCCCTGATCCGGGAGGGCAAGACCCACCAGCTCCGGAGCGTCATCCAGACGGGCGGCCAGTTCGGCATGATCACC
ATGGACGCCTGTCTGGCCGATCTCTACAAGCGCAAGCTCATCACCTACGAGCTGGGCCTCGCCCGGGCGGTGGACCCCAA
GGAGTTCATGCGCCTCGCTGGAGTGCAGGAGAAGCCCGCCCGCGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

67.529

96.133

0.649
  pilT Pseudomonas aeruginosa PAK

53.779

95.028

0.511
  pilT Acinetobacter baylyi ADP1

53.779

95.028

0.511
  pilT Pseudomonas stutzeri DSM 10701

53.488

95.028

0.508
  pilT Acinetobacter nosocomialis M2

53.043

95.304

0.506
  pilT Acinetobacter baumannii D1279779

53.043

95.304

0.506
  pilT Acinetobacter baumannii strain A118

53.043

95.304

0.506
  pilT Neisseria meningitidis 8013

52.663

93.37

0.492
  pilT Legionella pneumophila strain Lp02

51.163

95.028

0.486
  pilT Legionella pneumophila strain ERS1305867

51.163

95.028

0.486
  pilT Neisseria gonorrhoeae MS11

51.929

93.094

0.483
  pilT Vibrio cholerae strain A1552

53.191

90.884

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.191

90.884

0.483
  pilU Pseudomonas stutzeri DSM 10701

47.522

94.751

0.45
  pilU Acinetobacter baylyi ADP1

41.899

98.895

0.414
  pilU Vibrio cholerae strain A1552

45.484

85.635

0.39

Multiple sequence alignment