Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   TO73_RS00255 Genome accession   NZ_CP010822
Coordinates   51707..54376 (-) Length   889 a.a.
NCBI ID   WP_003046848.1    Uniprot ID   -
Organism   Thermus aquaticus Y51MC23     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 46707..59376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TO73_RS00235 (TO73_0054) - 47555..48184 (-) 630 WP_003046837.1 histidine phosphatase family protein -
  TO73_RS00240 (TO73_0055) purM 48181..49182 (-) 1002 WP_003046840.1 phosphoribosylformylglycinamidine cyclo-ligase -
  TO73_RS00245 (TO73_0056) gatB 49188..50597 (-) 1410 WP_003046842.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  TO73_RS00250 (TO73_0057) pilT 50607..51695 (-) 1089 WP_003046845.1 type IV pilus twitching motility protein PilT Machinery gene
  TO73_RS00255 (TO73_0058) pilF 51707..54376 (-) 2670 WP_003046848.1 type IV pilus assembly ATPase PilB Machinery gene
  TO73_RS00260 (TO73_0059) - 54373..54855 (-) 483 WP_003046850.1 YqeG family HAD IIIA-type phosphatase -
  TO73_RS00265 (TO73_0060) pgeF 54880..55614 (-) 735 WP_003046852.1 peptidoglycan editing factor PgeF -
  TO73_RS00270 (TO73_0061) - 55685..56089 (+) 405 WP_003046855.1 DUF4395 domain-containing protein -
  TO73_RS00275 (TO73_0062) ribH 56076..56549 (-) 474 WP_003046858.1 6,7-dimethyl-8-ribityllumazine synthase -
  TO73_RS00280 (TO73_0063) crcB 56570..56947 (+) 378 WP_003046862.1 fluoride efflux transporter CrcB -
  TO73_RS00285 (TO73_0064) - 56944..57276 (+) 333 WP_003046864.1 DUF190 domain-containing protein -
  TO73_RS00290 (TO73_0065) - 57321..57929 (+) 609 WP_003046867.1 sulfite oxidase-like oxidoreductase -
  TO73_RS00295 (TO73_0066) - 57926..58195 (+) 270 WP_003046870.1 hypothetical protein -
  TO73_RS00300 (TO73_0067) - 58171..58974 (-) 804 WP_003046873.1 histidinol-phosphatase HisJ family protein -

Sequence

Protein


Download         Length: 889 a.a.        Molecular weight: 98000.30 Da        Isoelectric Point: 5.0910

>NTDB_id=140092 TO73_RS00255 WP_003046848.1 51707..54376(-) (pilF) [Thermus aquaticus Y51MC23]
MSVLSIGDKRLGAILLDSGLLTDEELQMALERHREVGGSLAEVLVDMGLLSERRIAQAIEDHFGIPLVELHKMEIPPKVK
ALLPAEKAKELQAIPFALDEEAGVVRVAFVNPLDTLALEEVEDLTGLVVEPYQATKSDFLYALAKNYPELGLPLPPPPSG
PGQEELKLGEVLVKKGLISRDVLEEALVEQERTGDLLGRILVQKGLKEEDLYQALAEQKGLEFLPSTEGLTIDPAATSLL
LRSDALRYSAVPVAIREGKVEVVLADPRHLEAVEELLGRPARFFLTLPKAWEDLFHRAYPEKGRLGEVLVQEGRLSREGL
REALEVQKRLPKAKPLGEILVELGLVRPEDVEEALKKQRQGGGRLEDTLIQSGKLKPEALAQAVATQLGYPYINPEENPP
DPGAALLIPEDLARRYGVFPHHVEGKTLVLLMKDPRNILALDDVRLALKRKGLNYEVTPAVATEAAITKLIERFYGKEEL
GELAKELSKGYQQEEEAAIGELDESAAQKFVKQVIREAYLQDASDIHVEPRPTDVLVRLRVDGTLRQYTTLPKGALGPVI
SVIKIMGGLNIAEKRLPQDGRVRYREGTIDLDLRLSTLPTVYGEKAVMRLLKKAADIPEIEQLGFAPGVFERFQEVISKP
YGIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIR
DSETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVELFNISAALIGVLSQRLVRKICDHCKVEVKPDPEVLRRIGL
SEKEIQGAKLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARQKGMKTLREDGIYKALLGITTL
EEVLARTIE

Nucleotide


Download         Length: 2670 bp        

>NTDB_id=140092 TO73_RS00255 WP_003046848.1 51707..54376(-) (pilF) [Thermus aquaticus Y51MC23]
ATGAGCGTGCTCAGCATCGGCGACAAGCGGCTTGGCGCCATCCTTTTGGATTCCGGGCTCCTCACCGACGAGGAGCTCCA
GATGGCCCTAGAACGGCACCGGGAGGTGGGGGGATCCCTGGCCGAGGTCCTGGTGGACATGGGCCTTCTCTCGGAAAGGC
GCATCGCCCAGGCCATTGAGGATCACTTCGGCATCCCGCTGGTGGAGCTCCACAAGATGGAGATCCCACCCAAGGTGAAG
GCCCTCCTTCCCGCCGAAAAGGCCAAGGAGCTTCAGGCCATCCCCTTCGCCCTGGACGAGGAGGCCGGGGTGGTCCGGGT
GGCCTTCGTGAACCCCCTGGACACCCTGGCCCTCGAGGAGGTGGAGGACCTCACCGGCCTGGTGGTGGAGCCCTACCAGG
CCACCAAGAGCGACTTCCTCTACGCCTTGGCCAAGAACTACCCCGAGCTGGGCCTCCCTCTCCCCCCACCCCCCTCGGGC
CCCGGCCAGGAGGAGCTGAAGCTGGGGGAAGTTCTGGTGAAAAAAGGGCTCATAAGCCGGGATGTCTTAGAAGAAGCCCT
GGTGGAGCAGGAACGCACCGGGGACCTCCTGGGCAGGATCTTGGTACAAAAGGGACTCAAGGAGGAGGACCTCTACCAGG
CCCTGGCAGAGCAAAAGGGCCTGGAGTTCCTCCCCTCCACCGAGGGCCTCACCATAGACCCGGCGGCCACCTCCCTCCTC
CTCCGCTCCGATGCCCTTCGGTATAGCGCTGTGCCCGTGGCCATCCGCGAGGGAAAGGTGGAGGTGGTTCTGGCCGACCC
CCGGCACCTGGAGGCGGTGGAGGAGCTCTTGGGCCGCCCCGCCCGCTTTTTCCTCACGCTCCCCAAGGCCTGGGAGGACC
TCTTCCACCGGGCCTACCCGGAAAAGGGCAGGCTCGGAGAGGTCCTGGTGCAGGAGGGCCGCCTCTCCCGGGAAGGGCTC
AGGGAGGCCCTCGAGGTGCAAAAGCGCCTCCCCAAGGCCAAGCCCCTGGGGGAGATCCTGGTGGAGCTGGGCCTGGTCCG
GCCCGAGGACGTGGAGGAGGCCCTGAAGAAGCAGAGGCAGGGTGGAGGGCGCCTGGAGGACACCCTGATCCAGTCGGGGA
AGCTGAAGCCCGAGGCCCTGGCCCAGGCGGTGGCCACGCAACTGGGCTACCCCTACATCAACCCCGAGGAGAACCCCCCG
GACCCCGGGGCCGCCCTCCTCATCCCCGAGGACCTGGCCCGGCGCTACGGGGTCTTTCCCCACCACGTGGAGGGGAAGAC
CCTCGTCCTCCTCATGAAGGACCCCCGCAACATCCTGGCCCTGGACGACGTGCGCCTGGCCCTCAAGCGCAAGGGGCTTA
ACTACGAGGTCACCCCGGCGGTGGCCACCGAGGCGGCCATCACCAAGCTCATTGAGCGCTTCTACGGCAAGGAGGAGCTG
GGGGAGCTGGCCAAGGAGCTCTCCAAGGGCTACCAGCAGGAAGAGGAAGCGGCCATCGGGGAGCTGGACGAGAGCGCCGC
CCAGAAGTTCGTCAAGCAGGTCATCCGCGAGGCCTATTTGCAGGACGCTTCCGACATCCACGTAGAGCCCAGGCCCACCG
ATGTCCTGGTACGCCTCCGCGTGGACGGCACCCTGCGCCAGTACACCACCCTGCCCAAGGGGGCCCTGGGCCCGGTGATC
AGCGTCATCAAGATCATGGGCGGGCTCAACATCGCCGAGAAGCGCCTCCCCCAGGACGGCCGCGTGCGCTACCGGGAAGG
GACCATTGACCTGGACCTCCGCCTCTCCACCCTGCCCACCGTCTACGGGGAGAAGGCGGTGATGCGCCTCCTCAAGAAGG
CCGCCGACATCCCCGAAATAGAGCAGCTGGGCTTTGCTCCCGGAGTCTTTGAGCGCTTCCAGGAGGTGATCTCCAAGCCC
TACGGCATCTTCCTCATCACCGGCCCCACAGGGTCGGGCAAGAGCTTCACCACCTTCTCCATCCTGAAGCGCATCGCCAC
CCCCGATAAGAACACCCAGACCATTGAGGACCCGGTGGAGTACGAGATCCCCGGCATCAACCAGACCCAGGTGAACCCCC
AGGCGGGCCTCACCTTTGCCCGGGCCCTGAGGGCCTTCCTGCGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGC
GACTCGGAGACGGCCAAGATCGCCACCGAGGCCGCCCTCACCGGCCACCTGGTCATCGCCACCCTGCACACCAACGACGC
CGCCCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAGCTCTTCAACATCTCCGCCGCCCTTATCGGGGTCCTCTCCC
AGAGGCTGGTGCGGAAGATCTGCGACCACTGCAAGGTGGAGGTGAAGCCCGACCCCGAGGTCCTGAGGCGGATCGGCCTG
AGCGAAAAGGAGATCCAGGGGGCCAAGCTCTACAAGGGCATGGGGTGCGAGCGGTGCGGCGGCACAGGGTACAAGGGCCG
CTACGCCATCCACGAGCTTCTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCA
AGGAGATAGCCAGGCAAAAGGGCATGAAGACCCTGCGGGAGGACGGCATCTACAAAGCGCTCCTGGGCATCACCACCCTC
GAGGAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(200-303)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(369-479)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(513-779)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

86.727

100

0.867
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.629

100

0.567

Multiple sequence alignment