Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TK49_RS16345 Genome accession   NZ_CP010799
Coordinates   3471290..3472333 (-) Length   347 a.a.
NCBI ID   WP_045787652.1    Uniprot ID   A0A0D5ASY1
Organism   Ralstonia mannitolilytica strain SN82F48     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3466290..3477333
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TK49_RS16310 (TK49_16315) - 3466608..3466985 (+) 378 WP_045787645.1 CidA/LrgA family protein -
  TK49_RS16315 (TK49_16320) - 3466982..3467713 (+) 732 WP_045787646.1 LrgB family protein -
  TK49_RS16320 (TK49_16325) - 3467885..3468280 (+) 396 WP_045787647.1 VOC family protein -
  TK49_RS16325 (TK49_16330) - 3468344..3468823 (+) 480 WP_045787648.1 cupin domain-containing protein -
  TK49_RS16330 (TK49_16335) - 3468867..3469565 (+) 699 WP_045787649.1 helix-turn-helix transcriptional regulator -
  TK49_RS16335 (TK49_16340) - 3469582..3470031 (-) 450 WP_045787650.1 glutathione peroxidase -
  TK49_RS16340 (TK49_16345) pilU 3470097..3471236 (-) 1140 WP_045787651.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TK49_RS16345 (TK49_16350) pilT 3471290..3472333 (-) 1044 WP_045787652.1 type IV pilus twitching motility protein PilT Machinery gene
  TK49_RS16350 (TK49_16355) - 3472457..3473173 (+) 717 WP_045787653.1 YggS family pyridoxal phosphate-dependent enzyme -
  TK49_RS16355 (TK49_16360) proC 3473204..3474028 (+) 825 WP_045787654.1 pyrroline-5-carboxylate reductase -
  TK49_RS16360 (TK49_16365) - 3474061..3474336 (+) 276 WP_045787655.1 hypothetical protein -
  TK49_RS16365 (TK49_16370) ubiA 3474333..3475208 (-) 876 WP_045787938.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38264.01 Da        Isoelectric Point: 6.7828

>NTDB_id=139892 TK49_RS16345 WP_045787652.1 3471290..3472333(-) (pilT) [Ralstonia mannitolilytica strain SN82F48]
MDIAQLLAFSAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHAMVYDIMSDVQRKVYEENLEVDFSFEIPGLSR
FRVNAFNQNRGASAVFRTIPSKVLSLDDLKAPPVFAELAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNENDMGHILTVED
PIEFVHESKKSLINQRELGPHTLSFANALKSALREDPDVILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPSEEKDMVRTMLSESLEAVISQTLLKTRDGSGRIAAHEIMITTPAIRHLIRENKISQMYSMMQTSSGMGMQTLDQC
LADLIKRSAINYADARAIAKNPDAFAN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=139892 TK49_RS16345 WP_045787652.1 3471290..3472333(-) (pilT) [Ralstonia mannitolilytica strain SN82F48]
ATGGACATCGCGCAGCTTCTGGCCTTCTCGGCCAAGAACAAGGCGTCGGACCTGCATCTGTCGGCCGGCCTGCCGCCGAT
GATCCGGATCCACGGCGACATGCGCCGGATCAATGTGCCGCCGCTCACGCACCAGGATGTGCACGCCATGGTGTACGACA
TCATGAGCGACGTGCAGCGCAAGGTCTACGAAGAAAACCTGGAAGTCGACTTCTCGTTTGAGATTCCGGGCCTGTCCCGC
TTCCGGGTCAACGCGTTCAACCAGAACCGCGGCGCCTCGGCCGTGTTCCGGACGATTCCGTCGAAGGTGCTCTCGCTGGA
CGACCTGAAGGCGCCGCCCGTGTTTGCCGAGCTGGCGATGAAGCCGCGCGGCCTGGTGCTCGTGACCGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCCGCGATGGTGAACCACCGCAACGAAAACGACATGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACGAATCGAAGAAGAGCCTGATCAACCAGCGCGAACTCGGGCCGCACACGCTGTCGTTCGCCAA
TGCACTGAAGTCGGCGCTGCGTGAAGACCCGGACGTCATCCTCGTCGGCGAATTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACGGCCGCTGAAACCGGCCACTTGGTGTTCGCCACGCTGCACACGAGTTCTGCGGCCAAGACGATCGACCGGGTG
GTCGACGTGTTCCCGTCGGAAGAGAAAGACATGGTGCGGACCATGCTTTCCGAATCGCTGGAGGCGGTGATCTCGCAGAC
GCTGCTCAAGACGCGCGACGGCTCGGGGCGGATTGCGGCGCACGAGATCATGATCACGACGCCGGCCATCCGCCACCTGA
TCCGTGAGAACAAGATCTCGCAGATGTACTCGATGATGCAGACCAGCAGCGGCATGGGCATGCAGACGCTCGACCAGTGC
CTGGCCGACCTCATCAAGCGCTCGGCGATCAACTACGCGGATGCGCGCGCCATCGCCAAGAACCCCGACGCGTTCGCGAA
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D5ASY1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

71.014

99.424

0.706
  pilT Acinetobacter nosocomialis M2

71.014

99.424

0.706
  pilT Acinetobacter baumannii D1279779

71.014

99.424

0.706
  pilT Acinetobacter baumannii strain A118

71.014

99.424

0.706
  pilT Pseudomonas stutzeri DSM 10701

70.725

99.424

0.703
  pilT Acinetobacter baylyi ADP1

69.565

99.424

0.692
  pilT Neisseria gonorrhoeae MS11

68.497

99.712

0.683
  pilT Neisseria meningitidis 8013

68.497

99.712

0.683
  pilT Legionella pneumophila strain Lp02

67.826

99.424

0.674
  pilT Legionella pneumophila strain ERS1305867

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.875

97.118

0.64
  pilT Vibrio cholerae strain A1552

65.875

97.118

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.289

99.712

0.501
  pilU Acinetobacter baylyi ADP1

43.895

99.135

0.435
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403

Multiple sequence alignment