Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   TK49_RS16340 Genome accession   NZ_CP010799
Coordinates   3470097..3471236 (-) Length   379 a.a.
NCBI ID   WP_045787651.1    Uniprot ID   A0AAJ5D5Q9
Organism   Ralstonia mannitolilytica strain SN82F48     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3465097..3476236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TK49_RS16305 (TK49_16310) glcF 3465312..3466556 (+) 1245 WP_045787644.1 glycolate oxidase subunit GlcF -
  TK49_RS16310 (TK49_16315) - 3466608..3466985 (+) 378 WP_045787645.1 CidA/LrgA family protein -
  TK49_RS16315 (TK49_16320) - 3466982..3467713 (+) 732 WP_045787646.1 LrgB family protein -
  TK49_RS16320 (TK49_16325) - 3467885..3468280 (+) 396 WP_045787647.1 VOC family protein -
  TK49_RS16325 (TK49_16330) - 3468344..3468823 (+) 480 WP_045787648.1 cupin domain-containing protein -
  TK49_RS16330 (TK49_16335) - 3468867..3469565 (+) 699 WP_045787649.1 helix-turn-helix transcriptional regulator -
  TK49_RS16335 (TK49_16340) - 3469582..3470031 (-) 450 WP_045787650.1 glutathione peroxidase -
  TK49_RS16340 (TK49_16345) pilU 3470097..3471236 (-) 1140 WP_045787651.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TK49_RS16345 (TK49_16350) pilT 3471290..3472333 (-) 1044 WP_045787652.1 type IV pilus twitching motility protein PilT Machinery gene
  TK49_RS16350 (TK49_16355) - 3472457..3473173 (+) 717 WP_045787653.1 YggS family pyridoxal phosphate-dependent enzyme -
  TK49_RS16355 (TK49_16360) proC 3473204..3474028 (+) 825 WP_045787654.1 pyrroline-5-carboxylate reductase -
  TK49_RS16360 (TK49_16365) - 3474061..3474336 (+) 276 WP_045787655.1 hypothetical protein -
  TK49_RS16365 (TK49_16370) ubiA 3474333..3475208 (-) 876 WP_045787938.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42241.65 Da        Isoelectric Point: 6.8849

>NTDB_id=139891 TK49_RS16340 WP_045787651.1 3470097..3471236(-) (pilU) [Ralstonia mannitolilytica strain SN82F48]
MLDREAATKYIHELLQLMVNSRGSDLFITSDFPPAIKVDGKVTPISQQPLNATQAMSLVKAIMNEKQLREYEQTLECNFA
ITAPGAGRFRVSAFVQQGRAGMVLRTINTKIPSLDELDLPPILNDIVMSKRGLVIVVGATGSGKSTSLAAMVGHRNANSY
GHIITIEDPVEYVHAHQNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIINFFPEEKRQQLLIDLSLNLRAMIAQRLLPRKGKKGRVPAVEIMLATPLVQDLIFKGEVHELKEVMKKSREQ
GMISFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHSKRAGQTDLAAGTEHLNVV

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=139891 TK49_RS16340 WP_045787651.1 3470097..3471236(-) (pilU) [Ralstonia mannitolilytica strain SN82F48]
ATGCTGGACCGCGAAGCCGCCACCAAATACATCCACGAACTCCTGCAGTTGATGGTGAACAGCCGTGGCTCGGACTTGTT
CATCACGTCGGATTTCCCGCCCGCCATCAAGGTGGACGGCAAGGTCACGCCGATCTCGCAGCAGCCGTTGAACGCCACGC
AGGCCATGAGCCTGGTCAAGGCGATCATGAACGAGAAGCAGCTGCGCGAGTACGAACAGACCCTTGAGTGCAACTTCGCC
ATCACGGCCCCGGGTGCCGGGCGCTTTCGCGTGTCGGCCTTCGTGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACCAT
CAACACGAAGATCCCCTCGCTGGACGAGCTTGACCTGCCGCCGATCCTCAACGACATCGTGATGAGCAAGCGCGGCCTGG
TGATCGTGGTGGGCGCCACCGGCTCGGGCAAGTCGACCTCGCTGGCGGCCATGGTGGGGCATCGCAACGCCAATTCGTAC
GGCCATATCATCACGATCGAAGACCCGGTCGAATACGTGCACGCGCACCAGAACTGTGTGGTCACGCAGCGCGAGGTGGG
CGTGGACACCGAGTCGTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGATGTGATCCTGATCGGCGAAATCC
GCGATCGCGACACGATGGAATACGCCATCCAGTATGCGGAAACCGGCCACCTGTGCCTCGCCACGCTGCACGCCAACAGC
TCGAACCAGGCCATCGACCGCATCATCAACTTCTTTCCGGAAGAGAAGCGCCAGCAGTTGCTGATCGACCTCTCGCTGAA
CCTGCGCGCGATGATTGCGCAGCGCCTGCTGCCGCGCAAAGGCAAGAAGGGGCGCGTGCCGGCCGTGGAGATCATGCTGG
CCACGCCGCTGGTGCAGGACCTCATCTTCAAGGGCGAGGTGCACGAGCTGAAGGAGGTCATGAAGAAATCGCGCGAGCAG
GGCATGATTTCCTTCGACCAGGCGCTGTTCGAGCTGTACGAGGCGGACAAGATCACCTACGAAGACGCCCTGCGCAACGC
CGACTCGCTCAACGACCTGCGCCTGCAGATCAAGCTGCACAGCAAGCGCGCCGGGCAGACCGACCTGGCGGCCGGCACCG
AACATCTGAACGTGGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.674

93.931

0.57
  pilU Acinetobacter baylyi ADP1

55.714

92.348

0.515
  pilU Vibrio cholerae strain A1552

54.023

91.821

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.027

88.918

0.383
  pilT Acinetobacter baylyi ADP1

44.41

84.96

0.377
  pilT Legionella pneumophila strain Lp02

42.943

87.863

0.377
  pilT Legionella pneumophila strain ERS1305867

42.943

87.863

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.11

83.641

0.377
  pilT Vibrio cholerae strain A1552

45.11

83.641

0.377
  pilT Acinetobacter nosocomialis M2

44.201

84.169

0.372
  pilT Acinetobacter baumannii D1279779

44.201

84.169

0.372
  pilT Acinetobacter baumannii strain A118

44.201

84.169

0.372
  pilT Pseudomonas aeruginosa PAK

41.84

88.918

0.372
  pilT Pseudomonas stutzeri DSM 10701

41.84

88.918

0.372

Multiple sequence alignment