Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PG1C_RS13460 Genome accession   NZ_CP010554
Coordinates   2721211..2722254 (+) Length   347 a.a.
NCBI ID   WP_202635241.1    Uniprot ID   A0A0C5JBN6
Organism   Rugosibacter aromaticivorans strain Ca6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2716211..2727254
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG1C_RS13430 (PG1C_13220) - 2716401..2717372 (+) 972 WP_202635235.1 aspartate carbamoyltransferase catalytic subunit -
  PG1C_RS13435 (PG1C_13225) - 2717374..2718672 (+) 1299 WP_202635236.1 dihydroorotase -
  PG1C_RS13440 (PG1C_13230) - 2718732..2719028 (-) 297 WP_202635237.1 DUF167 domain-containing protein -
  PG1C_RS13445 (PG1C_13235) - 2719025..2719591 (-) 567 WP_202635238.1 YggT family protein -
  PG1C_RS13450 (PG1C_13240) proC 2719622..2720452 (-) 831 WP_202635239.1 pyrroline-5-carboxylate reductase -
  PG1C_RS13455 (PG1C_13245) - 2720467..2721165 (-) 699 WP_202635240.1 YggS family pyridoxal phosphate-dependent enzyme -
  PG1C_RS13460 (PG1C_13250) pilT 2721211..2722254 (+) 1044 WP_202635241.1 type IV pilus twitching motility protein PilT Machinery gene
  PG1C_RS13465 (PG1C_13255) pilU 2722294..2723430 (+) 1137 WP_202635242.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PG1C_RS13470 (PG1C_13260) - 2723603..2723986 (+) 384 WP_202635243.1 hypothetical protein -
  PG1C_RS13475 (PG1C_13265) - 2723997..2725178 (-) 1182 WP_202635244.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  PG1C_RS13480 (PG1C_13270) - 2725195..2725614 (-) 420 WP_202635245.1 CopD family protein -
  PG1C_RS13485 (PG1C_13275) - 2725602..2726474 (-) 873 WP_237218205.1 NAD-dependent epimerase/dehydratase family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38357.22 Da        Isoelectric Point: 7.2876

>NTDB_id=139188 PG1C_RS13460 WP_202635241.1 2721211..2722254(+) (pilT) [Rugosibacter aromaticivorans strain Ca6]
MDITELLSFVVKNNASDLHLSSGLPPMIRVHGDVRRINLPPMEHKEVHSLIYDIMNDGQRKVYEENLECDFSFAVPNLAR
FRVNAFVQNRGAAAVMRTIPSKILSLDDLKAPKIFAELADQPRGIVLVTGPTGSGKSTTLAAMVKHKNENEYGHILTVED
PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDVILVGEMRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKEMVRAMLSESLKAVISQTLCKTMDGTGRVAAHEIMIGTPAIRNLIREAKVAQMYSAIQTGQQFGMQTLDQN
LQELVKRRIISPAEARSKAANKDNFLG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=139188 PG1C_RS13460 WP_202635241.1 2721211..2722254(+) (pilT) [Rugosibacter aromaticivorans strain Ca6]
ATGGACATTACCGAGCTGCTCTCTTTCGTCGTCAAGAATAATGCGTCCGATTTACACCTTTCTTCAGGCTTGCCGCCGAT
GATTCGTGTCCATGGTGATGTGCGGCGGATCAATCTGCCGCCGATGGAACATAAAGAAGTGCACAGCCTGATCTATGACA
TCATGAATGACGGCCAGCGCAAGGTATATGAAGAAAATCTGGAATGTGACTTTTCTTTTGCTGTGCCCAATCTTGCGCGC
TTTCGCGTCAATGCCTTTGTCCAGAATCGTGGCGCGGCGGCGGTGATGCGCACCATCCCTTCAAAAATTCTGTCACTGGA
TGACTTGAAGGCACCAAAAATTTTTGCAGAACTCGCGGATCAGCCACGTGGCATTGTGTTGGTCACCGGGCCCACAGGCT
CCGGCAAATCAACCACGCTTGCCGCTATGGTCAAGCACAAGAATGAAAACGAGTACGGCCATATTCTGACCGTGGAAGAC
CCGATCGAATTCGTGCATGAATCGAAAAAATGCCTGATCAACCAGCGCGAAGTCGGCCCGCACACGCTCTCGTTCAACAA
CGCCCTGCGCAGCGCCCTGCGCGAAGACCCGGATGTCATTCTGGTTGGCGAAATGCGTGATCTGGAAACCATCCGCCTGG
CGCTATCAGCCGCAGAAACCGGCCACCTGGTGTTTGGCACATTGCACACCTCATCGGCTGCCAAAACCATCGACCGCATC
ATTGATGTGTTTCCAGCAGCAGAAAAGGAAATGGTGCGCGCCATGTTGTCCGAATCACTCAAAGCAGTCATTTCGCAAAC
TCTGTGCAAAACCATGGACGGCACGGGGCGCGTTGCCGCACACGAGATCATGATCGGCACGCCCGCCATCCGCAATCTGA
TCCGCGAGGCCAAGGTCGCCCAGATGTATTCCGCAATTCAGACCGGCCAGCAGTTTGGCATGCAGACGCTGGACCAGAAC
CTGCAAGAGCTGGTCAAGCGCCGCATTATTTCACCCGCCGAAGCGCGCAGCAAAGCGGCAAACAAGGATAACTTCCTCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C5JBN6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.59

99.712

0.764
  pilT Acinetobacter baumannii strain A118

76.59

99.712

0.764
  pilT Acinetobacter nosocomialis M2

76.59

99.712

0.764
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Pseudomonas aeruginosa PAK

74.493

99.424

0.741
  pilT Pseudomonas stutzeri DSM 10701

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

72.174

99.424

0.718
  pilT Legionella pneumophila strain ERS1305867

72.174

99.424

0.718
  pilT Neisseria meningitidis 8013

69.855

99.424

0.695
  pilT Neisseria gonorrhoeae MS11

69.565

99.424

0.692
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.751

97.406

0.66
  pilT Vibrio cholerae strain A1552

67.751

97.406

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

99.424

0.496
  pilU Acinetobacter baylyi ADP1

43.103

100

0.432
  pilU Pseudomonas stutzeri DSM 10701

43.21

93.372

0.403
  pilU Vibrio cholerae strain A1552

39.71

99.424

0.395

Multiple sequence alignment