Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PG1C_RS13465 Genome accession   NZ_CP010554
Coordinates   2722294..2723430 (+) Length   378 a.a.
NCBI ID   WP_202635242.1    Uniprot ID   A0A0C5JBC6
Organism   Rugosibacter aromaticivorans strain Ca6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2717294..2728430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG1C_RS13435 (PG1C_13225) - 2717374..2718672 (+) 1299 WP_202635236.1 dihydroorotase -
  PG1C_RS13440 (PG1C_13230) - 2718732..2719028 (-) 297 WP_202635237.1 DUF167 domain-containing protein -
  PG1C_RS13445 (PG1C_13235) - 2719025..2719591 (-) 567 WP_202635238.1 YggT family protein -
  PG1C_RS13450 (PG1C_13240) proC 2719622..2720452 (-) 831 WP_202635239.1 pyrroline-5-carboxylate reductase -
  PG1C_RS13455 (PG1C_13245) - 2720467..2721165 (-) 699 WP_202635240.1 YggS family pyridoxal phosphate-dependent enzyme -
  PG1C_RS13460 (PG1C_13250) pilT 2721211..2722254 (+) 1044 WP_202635241.1 type IV pilus twitching motility protein PilT Machinery gene
  PG1C_RS13465 (PG1C_13255) pilU 2722294..2723430 (+) 1137 WP_202635242.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PG1C_RS13470 (PG1C_13260) - 2723603..2723986 (+) 384 WP_202635243.1 hypothetical protein -
  PG1C_RS13475 (PG1C_13265) - 2723997..2725178 (-) 1182 WP_202635244.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  PG1C_RS13480 (PG1C_13270) - 2725195..2725614 (-) 420 WP_202635245.1 CopD family protein -
  PG1C_RS13485 (PG1C_13275) - 2725602..2726474 (-) 873 WP_237218205.1 NAD-dependent epimerase/dehydratase family protein -
  PG1C_RS13490 (PG1C_13280) - 2726522..2727568 (+) 1047 WP_202635247.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  PG1C_RS13495 (PG1C_13285) - 2727663..2728406 (-) 744 WP_202637082.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42300.71 Da        Isoelectric Point: 6.4465

>NTDB_id=139189 PG1C_RS13465 WP_202635242.1 2722294..2723430(+) (pilU) [Rugosibacter aromaticivorans strain Ca6]
MERDEGLKFMYQLLDMMMQKKGSDLFITAGFPPAMKIDGKMTPATTQVLSPQHTQELARAIMNDKQAAAFEETKEANFAI
SPSGIGRFRVNAFVQQGRVGIVLRTINVQIPDFDELKLPPVLKDVAMTKRGLVLFVGGTGSGKSTSLAAMIGYRNQNSHG
HIITIEDPVEYVHEHKNCIITQREVGVDTDSWEVALKNTLRQAPDVILIGEIREREVMEHAIAFAETGHLALGTLHANNS
NQALDRIINFFPEERRPQLLMDLSLNLKAVVSQRLIPLKEGKGRAAAVEVLLNSPLISDLIYKGDVHEIKEIMKKSRELG
MQTFDQALFDLHEEGRISYEDALRFADSMNEVRLMLKLHGRDAQTRDLHSGISHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=139189 PG1C_RS13465 WP_202635242.1 2722294..2723430(+) (pilU) [Rugosibacter aromaticivorans strain Ca6]
ATGGAACGCGATGAAGGTTTAAAGTTCATGTACCAGCTGTTGGACATGATGATGCAAAAAAAAGGGTCAGACTTATTCAT
TACTGCAGGCTTTCCGCCGGCGATGAAAATTGACGGCAAGATGACGCCGGCAACAACACAAGTTTTGTCGCCACAGCATA
CTCAGGAGCTGGCGCGCGCCATCATGAACGACAAGCAGGCTGCTGCATTTGAAGAAACCAAAGAAGCCAACTTCGCCATT
TCTCCATCCGGCATTGGCCGCTTTCGGGTTAATGCTTTTGTGCAACAAGGACGCGTAGGTATTGTGCTGCGGACCATCAA
TGTCCAGATTCCGGATTTCGATGAGCTCAAGCTGCCGCCGGTACTCAAAGATGTCGCCATGACCAAGCGTGGTCTGGTGC
TCTTCGTCGGCGGCACGGGTTCTGGTAAATCAACCTCGCTGGCGGCGATGATTGGCTACCGGAATCAGAACAGTCATGGC
CATATCATCACCATCGAAGATCCGGTGGAGTATGTGCACGAGCATAAGAACTGCATCATCACCCAGCGTGAAGTCGGCGT
CGATACCGACTCGTGGGAAGTAGCGCTGAAAAATACCCTTCGCCAAGCGCCGGACGTGATCCTGATTGGCGAAATTCGTG
AACGGGAAGTGATGGAACACGCCATCGCCTTCGCCGAAACCGGGCATCTTGCGCTAGGCACGCTGCATGCCAATAACTCG
AATCAGGCACTTGATCGCATCATCAATTTCTTCCCCGAGGAACGTCGCCCGCAGCTGTTGATGGATCTCTCTCTCAATCT
TAAGGCCGTGGTTTCGCAGCGCCTGATTCCGCTGAAAGAAGGTAAAGGCCGTGCAGCAGCCGTGGAAGTACTGCTCAACT
CGCCGCTGATATCCGATCTTATCTATAAAGGCGATGTGCACGAAATAAAGGAAATCATGAAAAAATCGCGCGAGCTTGGC
ATGCAGACTTTCGATCAAGCGTTGTTTGATTTGCACGAAGAAGGCCGTATCAGCTACGAAGATGCGCTACGCTTTGCAGA
CTCCATGAACGAAGTACGCCTGATGCTCAAACTGCATGGCAGAGATGCACAGACAAGGGATCTGCATTCCGGCATCAGCC
ACCTCGACATCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C5JBC6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.994

91.799

0.606
  pilU Acinetobacter baylyi ADP1

60.807

91.799

0.558
  pilU Vibrio cholerae strain A1552

58.96

91.534

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.413

88.36

0.384
  pilT Pseudomonas aeruginosa PAK

42.643

88.095

0.376
  pilT Acinetobacter baylyi ADP1

42.136

89.153

0.376
  pilT Legionella pneumophila strain Lp02

41.39

87.566

0.362
  pilT Legionella pneumophila strain ERS1305867

41.39

87.566

0.362
  pilT Acinetobacter nosocomialis M2

41.39

87.566

0.362
  pilT Acinetobacter baumannii D1279779

41.39

87.566

0.362
  pilT Acinetobacter baumannii strain A118

41.39

87.566

0.362

Multiple sequence alignment