Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RR42_RS17350 Genome accession   NZ_CP010536
Coordinates   3755168..3756211 (-) Length   347 a.a.
NCBI ID   WP_043349453.1    Uniprot ID   A0A0C4Y650
Organism   Cupriavidus basilensis strain 4G11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3750168..3761211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RR42_RS17320 (RR42_m3525) - 3751079..3751465 (+) 387 WP_043349434.1 CidA/LrgA family protein -
  RR42_RS17325 (RR42_m3526) - 3751462..3752184 (+) 723 WP_043349437.1 LrgB family protein -
  RR42_RS17330 (RR42_m3527) - 3752263..3752628 (+) 366 WP_173430689.1 VOC family protein -
  RR42_RS17335 (RR42_m3528) - 3752683..3753372 (+) 690 WP_043349443.1 helix-turn-helix transcriptional regulator -
  RR42_RS17340 (RR42_m3529) - 3753418..3753909 (-) 492 WP_043349446.1 glutathione peroxidase -
  RR42_RS17345 (RR42_m3530) pilU 3753994..3755133 (-) 1140 WP_043349449.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RR42_RS17350 (RR42_m3531) pilT 3755168..3756211 (-) 1044 WP_043349453.1 type IV pilus twitching motility protein PilT Machinery gene
  RR42_RS17355 (RR42_m3532) - 3756274..3757002 (+) 729 WP_043349456.1 YggS family pyridoxal phosphate-dependent enzyme -
  RR42_RS17360 (RR42_m3533) proC 3757058..3757882 (+) 825 WP_043349460.1 pyrroline-5-carboxylate reductase -
  RR42_RS17365 (RR42_m3534) ubiA 3757948..3758805 (-) 858 WP_052494817.1 4-hydroxybenzoate octaprenyltransferase -
  RR42_RS17370 (RR42_m3535) - 3758934..3759419 (-) 486 WP_043349465.1 Dps family protein -
  RR42_RS17375 (RR42_m3536) - 3759497..3759691 (-) 195 WP_043349470.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38404.11 Da        Isoelectric Point: 6.9771

>NTDB_id=139153 RR42_RS17350 WP_043349453.1 3755168..3756211(-) (pilT) [Cupriavidus basilensis strain 4G11]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAAMGHKDVHAMVYDIMSDTQRKQYEERLEIDFSFEIASLSR
FRVNAYNTQRGAAAVFRTIPSKVLSLEDLRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNDQDMGHILTVED
PIEFVHTSKKSLINQRELGPHTHSFANALRSALREDPDVVLVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRGIINYNDARAIAKNPDSFTG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=139153 RR42_RS17350 WP_043349453.1 3755168..3756211(-) (pilT) [Cupriavidus basilensis strain 4G11]
ATGGACATCGCGCAGCTATTAGCTTTCGCCGTCAAGAACAAGGCGTCCGATCTCCATCTCTCGGCAGACATGCCGCCGAT
GGTGCGTATCCACGGCGATATGCGCCGCATCAATGTGGCCGCGATGGGCCACAAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACACCCAGCGCAAGCAATATGAGGAACGGCTGGAAATCGACTTTTCCTTCGAGATCGCCAGCCTCTCGCGT
TTCCGGGTCAATGCCTACAACACGCAGCGCGGCGCCGCCGCCGTGTTCCGGACCATTCCTTCCAAGGTACTGTCGCTGGA
AGACCTGCGCGCGCCGGCGGTATTCGCTGACCTGTGCATGAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCTGCGATGGTGGACCACCGCAACGATCAGGACATGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAGTTCGTGCACACGTCCAAGAAGAGCCTGATCAACCAGCGCGAGCTGGGGCCGCACACGCATTCCTTCGCCAA
CGCCCTGCGCTCCGCGCTGCGTGAGGATCCCGACGTGGTGCTGGTGGGTGAGCTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCCGAAACGGGCCACCTGGTGTTTGCCACGCTGCACACGAGCTCGGCGGCCAAGACCATCGACCGGGTT
GTCGACGTGTTCCCGCCCGAAGAAAAAGACATGGTGCGCACCATGTTGTCGGAATCGCTGGAAGCGGTGATTTCGCAAAC
GCTGCTCAAGACGCGCGACGGCAACGGCCGCACGGCGGCCCACGAGATCATGATCGCCACACCAGCGATCCGCCACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGATGATGCAGACCAGCAGCGGACTTGGCATGCAGACGCTGGACCAGTGC
CTGTCGGACCTGATCAAGCGCGGCATCATCAACTACAACGATGCGCGCGCCATTGCCAAGAACCCCGACTCATTCACGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C4Y650

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.304

99.424

0.709
  pilT Acinetobacter baumannii D1279779

71.304

99.424

0.709
  pilT Acinetobacter baumannii strain A118

71.304

99.424

0.709
  pilT Acinetobacter baylyi ADP1

69.855

99.424

0.695
  pilT Pseudomonas stutzeri DSM 10701

69.275

99.424

0.689
  pilT Pseudomonas aeruginosa PAK

68.406

99.424

0.68
  pilT Legionella pneumophila strain Lp02

66.957

99.424

0.666
  pilT Legionella pneumophila strain ERS1305867

66.957

99.424

0.666
  pilT Neisseria gonorrhoeae MS11

66.763

99.712

0.666
  pilT Neisseria meningitidis 8013

66.763

99.712

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.985

97.118

0.631
  pilT Vibrio cholerae strain A1552

64.985

97.118

0.631
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.406

99.424

0.481
  pilU Acinetobacter baylyi ADP1

40.173

99.712

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.299

96.542

0.389
  pilU Vibrio cholerae strain A1552

39.701

96.542

0.383

Multiple sequence alignment