Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RR42_RS17345 Genome accession   NZ_CP010536
Coordinates   3753994..3755133 (-) Length   379 a.a.
NCBI ID   WP_043349449.1    Uniprot ID   A0A0C4YJM3
Organism   Cupriavidus basilensis strain 4G11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3748994..3760133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RR42_RS17315 (RR42_m3524) glcF 3749649..3750914 (+) 1266 WP_043349430.1 glycolate oxidase subunit GlcF -
  RR42_RS17320 (RR42_m3525) - 3751079..3751465 (+) 387 WP_043349434.1 CidA/LrgA family protein -
  RR42_RS17325 (RR42_m3526) - 3751462..3752184 (+) 723 WP_043349437.1 LrgB family protein -
  RR42_RS17330 (RR42_m3527) - 3752263..3752628 (+) 366 WP_173430689.1 VOC family protein -
  RR42_RS17335 (RR42_m3528) - 3752683..3753372 (+) 690 WP_043349443.1 helix-turn-helix transcriptional regulator -
  RR42_RS17340 (RR42_m3529) - 3753418..3753909 (-) 492 WP_043349446.1 glutathione peroxidase -
  RR42_RS17345 (RR42_m3530) pilU 3753994..3755133 (-) 1140 WP_043349449.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RR42_RS17350 (RR42_m3531) pilT 3755168..3756211 (-) 1044 WP_043349453.1 type IV pilus twitching motility protein PilT Machinery gene
  RR42_RS17355 (RR42_m3532) - 3756274..3757002 (+) 729 WP_043349456.1 YggS family pyridoxal phosphate-dependent enzyme -
  RR42_RS17360 (RR42_m3533) proC 3757058..3757882 (+) 825 WP_043349460.1 pyrroline-5-carboxylate reductase -
  RR42_RS17365 (RR42_m3534) ubiA 3757948..3758805 (-) 858 WP_052494817.1 4-hydroxybenzoate octaprenyltransferase -
  RR42_RS17370 (RR42_m3535) - 3758934..3759419 (-) 486 WP_043349465.1 Dps family protein -
  RR42_RS17375 (RR42_m3536) - 3759497..3759691 (-) 195 WP_043349470.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 41980.17 Da        Isoelectric Point: 6.3709

>NTDB_id=139152 RR42_RS17345 WP_043349449.1 3753994..3755133(-) (pilU) [Cupriavidus basilensis strain 4G11]
MLDRESAAKYINDLLELMVGNRGSDLFITADFPPAIKVDGKITPVSQQPLNPTQALGLVRSIMNERQAGEFDGSRECNFA
ISVPNAGRFRVSAFIQQGKAGMVVRTINTRIPSVEDLDLPPALHDIVMSKRGLVIVTGATGSGKSTSLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTDSWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRVVNFFPEEKRQQLLIDLSLNLKAMVSQRLLPRKGQKGRVPAVEIMLGTPLVADLIFKGEIHALKEVIKKSREQ
GMVSFDQALFDLYEAGKITYEDALRNADSLNDLRLMIKLHGTMDRETDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=139152 RR42_RS17345 WP_043349449.1 3753994..3755133(-) (pilU) [Cupriavidus basilensis strain 4G11]
ATGCTCGATCGCGAATCCGCTGCCAAGTACATCAACGACCTGCTCGAGCTGATGGTGGGCAACCGTGGCTCGGACCTGTT
CATCACCGCCGATTTTCCGCCGGCCATCAAGGTCGATGGCAAGATCACGCCAGTCTCGCAGCAGCCGCTCAACCCGACCC
AGGCGCTCGGCCTGGTGCGCTCCATCATGAACGAGCGGCAGGCGGGCGAGTTCGACGGCAGCCGCGAGTGCAACTTCGCC
ATCTCCGTGCCCAACGCCGGGCGCTTTCGCGTCTCAGCGTTCATCCAGCAGGGCAAGGCGGGCATGGTGGTGCGTACCAT
CAATACGCGCATTCCTTCGGTGGAAGACCTCGACCTGCCGCCCGCGCTGCATGACATCGTGATGTCAAAGCGCGGACTGG
TGATCGTCACCGGCGCCACCGGCTCGGGCAAGTCGACCTCGCTCGCGGCCATGCTCGATCACCGCAACGCGCATTCGTAC
GGGCACATCATCACCATCGAGGACCCGATCGAATACGTGCACGCGCACCAGAACTGCATCGTCACGCAGCGCGAGGTCGG
CATCGATACCGACTCCTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCC
GCGATCGGGAAACCATGGAGTACGCGATGCAGTACGCGGAAACGGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAT
GCCAACCAGGCCATCGATCGCGTGGTCAACTTCTTCCCCGAGGAAAAGCGCCAGCAGTTGCTGATCGACCTGTCGCTCAA
CCTGAAGGCGATGGTGTCGCAGCGCCTGTTGCCGCGCAAGGGGCAGAAGGGCCGGGTGCCGGCAGTGGAGATCATGCTCG
GCACGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGATCCATGCGCTCAAGGAAGTCATCAAGAAATCGCGCGAGCAG
GGCATGGTGTCGTTCGACCAGGCGCTCTTTGATCTCTACGAGGCTGGCAAGATCACCTACGAAGACGCGCTGCGCAATGC
CGACTCCCTCAACGACCTGCGCTTGATGATCAAGCTGCACGGCACCATGGACCGCGAGACCGACCTGGGCGCCGGCACCG
AACACCTGAACGTGATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C4YJM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.173

93.14

0.588
  pilU Acinetobacter baylyi ADP1

57.382

94.723

0.544
  pilU Vibrio cholerae strain A1552

53.719

95.778

0.515
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

88.918

0.398
  pilT Acinetobacter baylyi ADP1

43.363

89.446

0.388
  pilT Legionella pneumophila strain Lp02

44.144

87.863

0.388
  pilT Legionella pneumophila strain ERS1305867

44.144

87.863

0.388
  pilT Acinetobacter nosocomialis M2

43.844

87.863

0.385
  pilT Acinetobacter baumannii D1279779

43.844

87.863

0.385
  pilT Acinetobacter baumannii strain A118

43.844

87.863

0.385
  pilT Pseudomonas aeruginosa PAK

43.027

88.918

0.383
  pilT Pseudomonas stutzeri DSM 10701

43.027

88.918

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.641

0.361
  pilT Vibrio cholerae strain A1552

43.218

83.641

0.361

Multiple sequence alignment