Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Achr_RS19340 Genome accession   NZ_CP010415
Coordinates   4278538..4279572 (-) Length   344 a.a.
NCBI ID   WP_039806761.1    Uniprot ID   A0A0C4WU13
Organism   Azotobacter chroococcum NCIMB 8003     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4273538..4284572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Achr_RS19315 (Achr_38380) pyrR 4273987..4274496 (+) 510 WP_039806751.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  Achr_RS19320 (Achr_38390) - 4274515..4275531 (+) 1017 WP_039806752.1 aspartate carbamoyltransferase catalytic subunit -
  Achr_RS19325 (Achr_38400) - 4275531..4276802 (+) 1272 WP_039806753.1 dihydroorotase -
  Achr_RS19330 (Achr_38410) - 4276870..4277271 (-) 402 WP_039806755.1 NINE protein -
  Achr_RS19335 (Achr_38420) pilU 4277353..4278498 (-) 1146 WP_039806759.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Achr_RS19340 (Achr_38430) pilT 4278538..4279572 (-) 1035 WP_039806761.1 type IV pilus twitching motility protein PilT Machinery gene
  Achr_RS19345 (Achr_38440) - 4279644..4280336 (+) 693 WP_039806765.1 YggS family pyridoxal phosphate-dependent enzyme -
  Achr_RS19350 (Achr_38450) proC 4280354..4281172 (+) 819 WP_039806767.1 pyrroline-5-carboxylate reductase -
  Achr_RS19355 (Achr_38470) - 4281185..4281778 (+) 594 WP_039806769.1 YggT family protein -
  Achr_RS19360 - 4281778..4282077 (+) 300 WP_039806771.1 DUF167 domain-containing protein -
  Achr_RS19365 (Achr_38480) metX 4282232..4283371 (+) 1140 WP_039808304.1 homoserine O-succinyltransferase MetX -
  Achr_RS19370 (Achr_38490) metW 4283379..4283972 (+) 594 WP_039806774.1 methionine biosynthesis protein MetW -
  Achr_RS19375 (Achr_38500) - 4284043..4284462 (+) 420 WP_039806775.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37942.76 Da        Isoelectric Point: 7.0744

>NTDB_id=136998 Achr_RS19340 WP_039806761.1 4278538..4279572(-) (pilT) [Azotobacter chroococcum NCIMB 8003]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSRVLSMDDLGMGEVFKKITEVPRGLVLVTGPTGSGKSTTLAAMLDYLNANKHHHILTIED
PIEFVHESKKCLINQREVHRDTLGFAAALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSIGMQTLDMCL
KSLLSRGAITRENAREKAKIPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=136998 Achr_RS19340 WP_039806761.1 4278538..4279572(-) (pilT) [Azotobacter chroococcum NCIMB 8003]
ATGGACATTACCGAGCTGCTCGCCTTCAGCGCCAAGCAGGGCGCGTCGGACCTGCACCTCTCCGCCGGTCTGCCGCCGAT
GATCCGTGTCGACGGCGACGTGCGCCGGATCAACCTGCCGCCGATGGATCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAGTTCCTCGAGACCGACTTCTCCTTCGAGGTGCCGGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGTGCCGGCGCGGTGTTCCGGACCATTCCATCGCGGGTGCTGAGCATGGA
CGACCTGGGGATGGGCGAGGTGTTCAAGAAGATCACCGAGGTGCCGCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGTCGACCACCCTGGCGGCCATGCTCGACTACCTGAATGCCAACAAGCACCACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCTGGGCTTCGCCGC
GGCGCTGCGTTCGGCGCTGCGCGAGGACCCGGACGTCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCCGCGGAGGAGAAGTCGATGGTCCGCTCGATGCTCTCCGAATCGCTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAAGGTCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGCTCGATCGGCATGCAGACCCTGGACATGTGCCTG
AAGAGCCTGCTGTCCAGGGGCGCGATCACCCGCGAGAACGCCCGCGAGAAGGCGAAGATTCCCGACAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C4WU13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

91.86

100

0.919
  pilT Pseudomonas aeruginosa PAK

91.279

100

0.913
  pilT Acinetobacter nosocomialis M2

82.849

100

0.828
  pilT Acinetobacter baumannii D1279779

82.558

100

0.826
  pilT Acinetobacter baumannii strain A118

82.558

100

0.826
  pilT Acinetobacter baylyi ADP1

80.814

100

0.808
  pilT Legionella pneumophila strain Lp02

73.964

98.256

0.727
  pilT Legionella pneumophila strain ERS1305867

73.964

98.256

0.727
  pilT Neisseria meningitidis 8013

69.412

98.837

0.686
  pilT Neisseria gonorrhoeae MS11

69.118

98.837

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.824

98.837

0.68
  pilT Vibrio cholerae strain A1552

68.824

98.837

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Vibrio cholerae strain A1552

39.528

98.547

0.39

Multiple sequence alignment