Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Achr_RS19335 Genome accession   NZ_CP010415
Coordinates   4277353..4278498 (-) Length   381 a.a.
NCBI ID   WP_039806759.1    Uniprot ID   A0A0C4WRI3
Organism   Azotobacter chroococcum NCIMB 8003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4272353..4283498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Achr_RS19305 (Achr_38360) - 4272951..4273517 (+) 567 WP_377802447.1 YqgE/AlgH family protein -
  Achr_RS19310 (Achr_38370) ruvX 4273514..4273942 (+) 429 WP_039806749.1 Holliday junction resolvase RuvX -
  Achr_RS19315 (Achr_38380) pyrR 4273987..4274496 (+) 510 WP_039806751.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  Achr_RS19320 (Achr_38390) - 4274515..4275531 (+) 1017 WP_039806752.1 aspartate carbamoyltransferase catalytic subunit -
  Achr_RS19325 (Achr_38400) - 4275531..4276802 (+) 1272 WP_039806753.1 dihydroorotase -
  Achr_RS19330 (Achr_38410) - 4276870..4277271 (-) 402 WP_039806755.1 NINE protein -
  Achr_RS19335 (Achr_38420) pilU 4277353..4278498 (-) 1146 WP_039806759.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Achr_RS19340 (Achr_38430) pilT 4278538..4279572 (-) 1035 WP_039806761.1 type IV pilus twitching motility protein PilT Machinery gene
  Achr_RS19345 (Achr_38440) - 4279644..4280336 (+) 693 WP_039806765.1 YggS family pyridoxal phosphate-dependent enzyme -
  Achr_RS19350 (Achr_38450) proC 4280354..4281172 (+) 819 WP_039806767.1 pyrroline-5-carboxylate reductase -
  Achr_RS19355 (Achr_38470) - 4281185..4281778 (+) 594 WP_039806769.1 YggT family protein -
  Achr_RS19360 - 4281778..4282077 (+) 300 WP_039806771.1 DUF167 domain-containing protein -
  Achr_RS19365 (Achr_38480) metX 4282232..4283371 (+) 1140 WP_039808304.1 homoserine O-succinyltransferase MetX -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42892.14 Da        Isoelectric Point: 6.5770

>NTDB_id=136997 Achr_RS19335 WP_039806759.1 4277353..4278498(-) (pilU) [Azotobacter chroococcum NCIMB 8003]
MEFEKLLRLMMEKGASDLFITAGMPPSMKLNGRLVPVTRNPLSPEQARETVFAVMNEQQRREFAACHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETRIPTFDELQLPEVLRELSMTKRGLVLFVGATGTGKSTSLAAMIGYRNQNSSGHIISIED
PIEFIHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVIMVGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPADRHHQVWMDLSLNLRAIVAQQLIPTPDGKGRRAVIEVLINTPLVADLIRKGEVHELKAVMKRSTEQGMQTFDQA
LYQLYSQGEITYEEALRHADSANDLRLMIKLESETDSRHLESVADGLSLQASEEDTGRRMR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=136997 Achr_RS19335 WP_039806759.1 4277353..4278498(-) (pilU) [Azotobacter chroococcum NCIMB 8003]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGATGGAGAAGGGCGCTTCCGATCTGTTCATCACCGCGGGCATGCCGCCGTC
GATGAAGCTCAACGGGCGCCTGGTGCCGGTAACCCGCAACCCGCTGTCGCCGGAGCAGGCCCGGGAAACCGTGTTCGCGG
TGATGAACGAACAGCAGCGCCGCGAGTTCGCCGCCTGCCACGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGCGCCTTCTACCAGCGCAACCTGGCGGGGATGGTGCTGCGGCGCATCGAGACGCGCATCCCGACCTTCGA
CGAGCTCCAGCTGCCCGAGGTGCTGCGCGAGCTGTCGATGACCAAGCGCGGCCTGGTGCTGTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATTGGCTACCGCAACCAGAACAGCAGCGGCCACATCATCTCCATCGAGGAC
CCGATCGAGTTCATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGCATCGACACCGAGTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGGTCGGCGAGATCCGCACCCGCGAAACCATGGACTACG
CGGTGGCCTTCGCCGAGACAGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTCGACCGGATC
ATCAACTTCTTCCCCGCCGACCGTCACCACCAGGTGTGGATGGACCTGTCGCTGAACCTGCGCGCCATCGTCGCCCAGCA
GCTGATCCCGACGCCGGACGGCAAGGGGCGCCGGGCGGTGATCGAGGTGCTGATCAATACCCCGCTGGTCGCCGACCTGA
TCCGCAAGGGCGAGGTCCACGAGCTCAAGGCGGTGATGAAGCGCTCCACCGAGCAAGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACAGCCAGGGCGAGATCACCTACGAGGAAGCCCTGCGCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTGGAGTCGGAGACCGACAGCCGGCACCTGGAGAGCGTGGCCGACGGGCTCAGCCTGCAGGCCAGCGAGG
AGGACACCGGCCGGCGCATGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C4WRI3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

83.465

100

0.835
  pilU Acinetobacter baylyi ADP1

64.146

93.701

0.601
  pilU Vibrio cholerae strain A1552

59.831

93.438

0.559
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

88.976

0.391
  pilT Pseudomonas aeruginosa PAK

41.194

87.927

0.362

Multiple sequence alignment