Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   SB85_RS18165 Genome accession   NZ_CP010409
Coordinates   4479829..4480248 (+) Length   139 a.a.
NCBI ID   WP_043095006.1    Uniprot ID   -
Organism   Xanthomonas sacchari strain R1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4474829..4485248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SB85_RS18145 (SB85_18215) - 4475246..4476580 (+) 1335 WP_043095003.1 HAMP domain-containing sensor histidine kinase -
  SB85_RS18150 (SB85_18220) coaE 4476691..4477320 (-) 630 WP_043095004.1 dephospho-CoA kinase -
  SB85_RS18155 (SB85_18225) - 4477334..4478197 (-) 864 WP_017910397.1 A24 family peptidase -
  SB85_RS18160 (SB85_18230) pilC 4478204..4479463 (-) 1260 WP_043095005.1 type II secretion system F family protein Machinery gene
  SB85_RS18165 (SB85_18235) pilA/pilAI 4479829..4480248 (+) 420 WP_043095006.1 pilin Machinery gene
  SB85_RS18170 (SB85_18240) pilB 4480795..4482528 (+) 1734 WP_043095007.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SB85_RS18175 (SB85_18245) pilR 4482694..4484082 (-) 1389 WP_043095008.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14542.71 Da        Isoelectric Point: 8.0971

>NTDB_id=136950 SB85_RS18165 WP_043095006.1 4479829..4480248(+) (pilA/pilAI) [Xanthomonas sacchari strain R1]
MKKQQGFTLIELMIVVAIIAILAAIAIPMYQDYVVKSQATSALAEITPGKVGFETAINQGKTPSTTATDDGYIGIPATTT
YCNVTLDATATGNITCAAKGGNAGKFNTKKIIWTRDANGAWTCTSDLDAKYRPGSCAAV

Nucleotide


Download         Length: 420 bp        

>NTDB_id=136950 SB85_RS18165 WP_043095006.1 4479829..4480248(+) (pilA/pilAI) [Xanthomonas sacchari strain R1]
ATGAAGAAGCAGCAGGGCTTTACCCTGATCGAACTGATGATCGTTGTCGCGATCATTGCCATCCTGGCCGCCATCGCAAT
CCCGATGTACCAGGACTATGTGGTCAAGTCCCAGGCGACCTCGGCTCTGGCTGAAATCACGCCTGGGAAGGTCGGTTTTG
AGACCGCGATCAACCAGGGCAAGACTCCCAGCACCACTGCGACGGATGATGGCTACATCGGCATTCCGGCCACCACCACC
TATTGCAACGTTACGCTGGATGCCACCGCTACTGGCAACATCACTTGCGCCGCCAAGGGCGGGAACGCCGGTAAATTCAA
CACCAAGAAGATCATCTGGACGCGTGATGCCAATGGTGCTTGGACTTGCACCAGTGATCTGGACGCTAAGTATCGTCCGG
GCAGCTGCGCTGCAGTTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

68.657

96.403

0.662
  pilA/pilAII Pseudomonas stutzeri DSM 10701

60.606

94.964

0.576
  pilA Pseudomonas aeruginosa PAK

46.309

100

0.496
  pilA Acinetobacter baumannii strain A118

48.252

100

0.496
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.537

100

0.46
  pilA Vibrio cholerae strain A1552

43.537

100

0.46
  pilA Vibrio cholerae C6706

43.537

100

0.46
  pilA2 Legionella pneumophila str. Paris

45.652

99.281

0.453
  pilA2 Legionella pneumophila strain ERS1305867

45.652

99.281

0.453
  comP Acinetobacter baylyi ADP1

40

100

0.446
  pilA Ralstonia pseudosolanacearum GMI1000

36.747

100

0.439
  pilA Vibrio parahaemolyticus RIMD 2210633

38.686

98.561

0.381
  pilE Neisseria gonorrhoeae MS11

33.974

100

0.381
  pilE Neisseria gonorrhoeae strain FA1090

32.911

100

0.374

Multiple sequence alignment