Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SB85_RS18170 Genome accession   NZ_CP010409
Coordinates   4480795..4482528 (+) Length   577 a.a.
NCBI ID   WP_043095007.1    Uniprot ID   -
Organism   Xanthomonas sacchari strain R1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4475795..4487528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SB85_RS18150 (SB85_18220) coaE 4476691..4477320 (-) 630 WP_043095004.1 dephospho-CoA kinase -
  SB85_RS18155 (SB85_18225) - 4477334..4478197 (-) 864 WP_017910397.1 A24 family peptidase -
  SB85_RS18160 (SB85_18230) pilC 4478204..4479463 (-) 1260 WP_043095005.1 type II secretion system F family protein Machinery gene
  SB85_RS18165 (SB85_18235) pilA/pilAI 4479829..4480248 (+) 420 WP_043095006.1 pilin Machinery gene
  SB85_RS18170 (SB85_18240) pilB 4480795..4482528 (+) 1734 WP_043095007.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SB85_RS18175 (SB85_18245) pilR 4482694..4484082 (-) 1389 WP_043095008.1 sigma-54 dependent transcriptional regulator Regulator
  SB85_RS18180 (SB85_18250) - 4484165..4485778 (-) 1614 WP_043095009.1 ATP-binding protein -
  SB85_RS18185 (SB85_18255) sucC 4486170..4487339 (+) 1170 WP_010342227.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62582.91 Da        Isoelectric Point: 5.6425

>NTDB_id=136951 SB85_RS18170 WP_043095007.1 4480795..4482528(+) (pilB) [Xanthomonas sacchari strain R1]
MSALMAPNLVGITGIARRLVQDGALDEAAARAAMAQAGEAKVPLPQWFSEKKLVTAAQLAAANAVEFGMPLMDVSVFDAS
QNAMKLVSEELLQKYQVLPLFKRGGRLFVGISNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDSLGSALG
DDEGMDNLDVGGGDEDMGGGGDTGIDAKGEDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKNKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPVQLPE
KALLAEGFTEAEIAAGVQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIASAAQKIGVKDLRQSALLK
ARNGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=136951 SB85_RS18170 WP_043095007.1 4480795..4482528(+) (pilB) [Xanthomonas sacchari strain R1]
ATGAGCGCGTTGATGGCTCCAAATCTTGTCGGCATCACCGGCATCGCCCGCCGCCTCGTGCAGGACGGCGCGCTGGACGA
AGCCGCGGCGCGGGCCGCGATGGCCCAGGCGGGCGAGGCCAAGGTGCCGCTGCCGCAGTGGTTCTCCGAGAAGAAGCTGG
TCACCGCCGCGCAGTTGGCCGCCGCCAATGCGGTGGAGTTCGGCATGCCGTTGATGGATGTCTCGGTTTTCGATGCCAGC
CAGAACGCAATGAAGCTGGTCAGCGAGGAGTTGCTGCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCGGCCGCCT
GTTCGTCGGCATCAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCACACCAATCTGACGGTGGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGCCGGACCCTGGAGCAGTGGCAGGCCAGCAACGATTCCCTCGGCAGCGCGCTGGGC
GACGACGAGGGCATGGACAACCTCGATGTCGGCGGCGGCGACGAGGACATGGGCGGCGGTGGCGACACCGGCATCGACGC
CAAGGGGGAGGACACCCCGGTCGTCAAGTTCGTGAACAAGGTGCTGGTCGATGCGATCCGCCGCGGCGCGTCCGACATCC
ACTTCGAGCCCTACGAGGACGACTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAAGGCGCCGGTC
AAGCTCAATCAGCGCATCGCCGCGCGCCTCAAGGTGATGGCGCAGTTGGACATCGCCGAGAAGCGGGTCCCGCAGGACGG
CCGCATCAAGCTCAATCTGTCCAAGAACAAGCAGATCGATTTCCGCGTCAGCACCCTGCCCACGCTGTTCGGCGAGAAGA
TCGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAGCTG
TTCCTGGACGCGATCCACAAGCCGTACGGCATGGTCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTA
CACCGCGCTGGGCATCCTCAACGACGATACCCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATCCGCCTGCCCGGGG
TCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCGGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGAC
ATCATCATGGTTGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGTCACATGGTGCT
GTCCACCCTGCACACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCA
GTTCGGTGACCCTGGTCATCGCCCAGCGCCTGGCGCGGCGCCTTTGCAACAACTGCAAGCGCCCGGTCCAGTTGCCGGAA
AAGGCCTTGCTGGCCGAAGGCTTCACCGAGGCGGAGATCGCCGCCGGCGTCCAACTGTACGAGGCGGTCGGCTGCGACGA
GTGCACCGAGGGCTACAAAGGCCGCACCGGCATCTACCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGCTGC
AGGGCGGCAACGCCATGGACATCGCCAGCGCCGCGCAGAAGATCGGCGTCAAGGACCTGCGCCAGTCGGCCCTGCTCAAG
GCCCGCAACGGCATCACCAGCCTGGCCGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.844

100

0.549
  pilB Acinetobacter baumannii D1279779

54.467

100

0.549
  pilB Legionella pneumophila strain ERS1305867

51.142

98.614

0.504
  pilB Vibrio cholerae strain A1552

49.74

100

0.497
  pilF Neisseria gonorrhoeae MS11

49.296

98.44

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

46.046

98.614

0.454
  pilB Vibrio campbellii strain DS40M4

45.694

98.614

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

36.721

100

0.388
  pilF Thermus thermophilus HB27

39.619

90.988

0.36

Multiple sequence alignment