Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PN96_RS00850 Genome accession   NZ_CP009977
Coordinates   165860..166900 (+) Length   346 a.a.
NCBI ID   WP_014233032.1    Uniprot ID   -
Organism   Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 160860..171900
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PN96_RS00820 (PN96_00805) - 162035..162637 (-) 603 WP_014233038.1 XTP/dITP diphosphatase -
  PN96_RS00825 (PN96_00810) - 162771..163202 (-) 432 WP_020334002.1 DUF4426 domain-containing protein -
  PN96_RS00830 (PN96_00815) yggU 163277..163567 (-) 291 WP_014233036.1 DUF167 family protein YggU -
  PN96_RS00835 (PN96_00820) - 163567..164124 (-) 558 WP_014233035.1 YggT family protein -
  PN96_RS00840 (PN96_00825) proC 164177..164995 (-) 819 WP_020334000.1 pyrroline-5-carboxylate reductase -
  PN96_RS00845 (PN96_00830) - 165118..165831 (-) 714 WP_020333999.1 YggS family pyridoxal phosphate-dependent enzyme -
  PN96_RS00850 (PN96_00835) pilT 165860..166900 (+) 1041 WP_014233032.1 type IV pilus twitching motility protein PilT Machinery gene
  PN96_RS00855 (PN96_00840) pilU 166931..168037 (+) 1107 WP_014233031.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PN96_RS00860 (PN96_00845) ruvX 168103..168528 (-) 426 WP_014233030.1 Holliday junction resolvase RuvX -
  PN96_RS00865 (PN96_00850) - 168570..169133 (-) 564 WP_014233029.1 YqgE/AlgH family protein -
  PN96_RS00875 (PN96_00855) gshB 169513..170463 (-) 951 WP_020333997.1 glutathione synthase -
  PN96_RS00880 (PN96_00860) rsmE 170478..171209 (-) 732 WP_020333996.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38247.54 Da        Isoelectric Point: 6.3867

>NTDB_id=133309 PN96_RS00850 WP_014233032.1 165860..166900(+) (pilT) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELQNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLDQLEAPEIFSKIASGQKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLSSVIAQKLLKRNGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLLAQGIVSHEEADSKVGLDVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=133309 PN96_RS00850 WP_014233032.1 165860..166900(+) (pilT) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCCTCAGATCTACATCTTTCTGCAGGTGTACCACCTAT
GGTCAGAATTGATGGCGATGTGAGAAAGCTCGGGATTCCAGCGTTTACACACCAAGAGGTTCATCGCTTGGTATTCGAAA
TCATGAATGACGCTCAACGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTTTCTTTTGAACTACAGAACGTTGGTCGC
TTTCGTGTCAACGCCTTTAACCAGTCCCGTGGCTGCGCAGCGGTATTTCGTACCATTCCAAGCAGCATACCAACGCTTGA
TCAACTCGAAGCTCCAGAAATCTTCAGCAAGATTGCCAGCGGGCAAAAAGGCTTAGTTCTGGTAACCGGACCAACCGGCT
CTGGTAAATCGACGACTCTAGCTGCCATTGTCGACTACATCAATCGTAATCACAATAAGCACATTCTCACAATTGAAGAT
CCAATTGAATTTGTTCATACCAACAACAAATGTTTGATCAACCAGCGTGAAGTTCACCGTGACACTCATAGCTTCCAAAA
CGCCTTACGCAGTGCGTTGCGTGAAGACCCGGATGTCATCTTAGTTGGTGAGATGCGTGATAAAGAAACCATCAGCCTTG
CATTGACCGCCGCAGAAACCGGTCATTTGGTTTTTGGAACGCTACACACCAGCTCAGCCGCAAAAACCATCGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGACATGGTGCGTTCAATGTTGTCAGAATCGTTGAGTTCAGTCATTGCCCAGAA
GTTACTTAAGCGTAATGGTGGTGGTCGAGTCGCCTGCCATGAAATCATGATGGCGACACCAGCAATCCGTAATTTGATTC
GCGAAGATAAAGTCGCACAGATGTACTCGATAATTCAAACTGGTGCGGCACACGGCATGCAGACTATGGAGCAAAATGCT
CGTCAGCTATTGGCGCAAGGTATTGTTTCTCACGAAGAGGCCGACAGCAAAGTTGGGCTTGATGTACAGCAGTTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.667

99.711

0.864
  pilT Vibrio cholerae strain A1552

86.667

99.711

0.864
  pilT Acinetobacter baumannii D1279779

71.856

96.532

0.694
  pilT Acinetobacter baumannii strain A118

71.856

96.532

0.694
  pilT Acinetobacter nosocomialis M2

71.557

96.532

0.691
  pilT Acinetobacter baylyi ADP1

71.818

95.376

0.685
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.399

0.679
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Legionella pneumophila strain Lp02

67.742

98.555

0.668
  pilT Legionella pneumophila strain ERS1305867

67.742

98.555

0.668
  pilT Neisseria meningitidis 8013

65.373

96.821

0.633
  pilT Neisseria gonorrhoeae MS11

65.075

96.821

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.991

100

0.491
  pilU Vibrio cholerae strain A1552

41.194

96.821

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.821

0.393
  pilU Acinetobacter baylyi ADP1

38.393

97.11

0.373

Multiple sequence alignment