Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PN96_RS00855 Genome accession   NZ_CP009977
Coordinates   166931..168037 (+) Length   368 a.a.
NCBI ID   WP_014233031.1    Uniprot ID   A0AAN0Y4F4
Organism   Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 161931..173037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PN96_RS00820 (PN96_00805) - 162035..162637 (-) 603 WP_014233038.1 XTP/dITP diphosphatase -
  PN96_RS00825 (PN96_00810) - 162771..163202 (-) 432 WP_020334002.1 DUF4426 domain-containing protein -
  PN96_RS00830 (PN96_00815) yggU 163277..163567 (-) 291 WP_014233036.1 DUF167 family protein YggU -
  PN96_RS00835 (PN96_00820) - 163567..164124 (-) 558 WP_014233035.1 YggT family protein -
  PN96_RS00840 (PN96_00825) proC 164177..164995 (-) 819 WP_020334000.1 pyrroline-5-carboxylate reductase -
  PN96_RS00845 (PN96_00830) - 165118..165831 (-) 714 WP_020333999.1 YggS family pyridoxal phosphate-dependent enzyme -
  PN96_RS00850 (PN96_00835) pilT 165860..166900 (+) 1041 WP_014233032.1 type IV pilus twitching motility protein PilT Machinery gene
  PN96_RS00855 (PN96_00840) pilU 166931..168037 (+) 1107 WP_014233031.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PN96_RS00860 (PN96_00845) ruvX 168103..168528 (-) 426 WP_014233030.1 Holliday junction resolvase RuvX -
  PN96_RS00865 (PN96_00850) - 168570..169133 (-) 564 WP_014233029.1 YqgE/AlgH family protein -
  PN96_RS00875 (PN96_00855) gshB 169513..170463 (-) 951 WP_020333997.1 glutathione synthase -
  PN96_RS00880 (PN96_00860) rsmE 170478..171209 (-) 732 WP_020333996.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PN96_RS00885 (PN96_00865) endA 171349..172044 (-) 696 WP_020333995.1 deoxyribonuclease I -
  PN96_RS00890 (PN96_00870) - 172176..172673 (-) 498 WP_020333994.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41300.35 Da        Isoelectric Point: 6.2478

>NTDB_id=133310 PN96_RS00855 WP_014233031.1 166931..168037(+) (pilU) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
MDLNKFLEGMLALQASDLYITVGAPILFRVDGELRPQGDKLTENDVAQLLDNAMDPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETDIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKSGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEPLATGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=133310 PN96_RS00855 WP_014233031.1 166931..168037(+) (pilU) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
ATGGATCTGAATAAATTTCTTGAAGGTATGCTGGCGCTGCAAGCGTCGGATCTTTATATCACGGTCGGCGCGCCGATTTT
GTTTCGTGTCGACGGCGAGTTACGTCCGCAAGGTGACAAGCTGACAGAAAATGATGTGGCGCAGTTGCTGGATAATGCAA
TGGATCCTGAGCGTCGTCAGGAGTTTCGAAAAAGTCGAGAGTCTAACTTTGCTATCGTCAGAGATTGTGGTCGATTCCGT
GTCAGTGCCTTTTTCCAGAGAGAATTGCCGGGCGCGGTTATTCGCCGTATTGAGACCGACATCCCTACCTTTGAGCAGCT
CAAACTGCCTCTGGTGTTGCAAGATTTAGCGATAGCCAAGAGAGGTCTGGTGCTGGTGGTTGGTGCGACTGGCTCTGGTA
AGTCGACCACAATGGCGGCGATGACAGGCTACCGAAACAGTAATAAGACCGGGCATATCCTGACGGTAGAAGATCCGATC
GAATTTGTTCATGAACACCAGCGTTGTATCGTAACCCAGCGTGAAGTCGGTTTAGACACCGAAAGTTACGAAGTGGCACT
GAAAAACTCCCTGCGACAGGCTCCAGATATGATTTTGATCGGCGAAATCCGCAGTAGAGAGACGATGGAATATGCGATGA
CTTTTGCTGAAACCGGGCATTTGTGTATGGCGACTCTCCATGCTAACAACGCCAACCAGGCATTAGAGCGGATTCTTCAT
TTAGTACCTAAAGATCAAAAAGAGCAGTTCTTGTTTGATCTGTCTATGAACTTAAAAGGCGTGGTTGGTCAGCAGTTGAT
CCGCGATAAGAGTGGGCAGGGACGTCATGGCGTATTTGAGATCCTGCTGAACAGCCCGCGTGTTTCGGATTTAATCCGTC
GTGGCGATCTGCATGAACTTAAGTCGACGATGGCTCGTTCAAATGAGTTTGGTATGCTGACTTTTGACCAGTCGCTTTAC
AAGTTAGTGATGCAAGGCAAAATCAGTGAAGAAGATGCACTGCACAGTGCTGATTCGGCAAATGACCTGCGTCTCATGCT
GAAGACCCAACGCGGTGAGCCTCTGGCAACAGGAAGCTTGGCGAACGTTAAGATTGATATGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.522

100

0.815
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

54.986

95.38

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44

88.315

0.389
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Acinetobacter baylyi ADP1

40.988

93.478

0.383
  pilT Acinetobacter baumannii D1279779

41.298

92.12

0.38
  pilT Acinetobacter baumannii strain A118

41.298

92.12

0.38
  pilT Acinetobacter nosocomialis M2

41.298

92.12

0.38
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

40.351

92.935

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372

Multiple sequence alignment