Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LT85_RS04225 Genome accession   NZ_CP009962
Coordinates   943593..944636 (-) Length   347 a.a.
NCBI ID   WP_038485669.1    Uniprot ID   A0A0A1F8J4
Organism   Collimonas arenae strain Cal35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 938593..949636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LT85_RS04205 (LT85_0830) glpK 939545..941041 (+) 1497 WP_038485657.1 glycerol kinase GlpK -
  LT85_RS04210 (LT85_0831) - 941132..941845 (+) 714 WP_038485660.1 MIP/aquaporin family protein -
  LT85_RS04215 (LT85_0832) - 941906..942409 (-) 504 WP_038485663.1 glutathione peroxidase -
  LT85_RS04220 (LT85_0833) pilU 942406..943542 (-) 1137 WP_038485666.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LT85_RS04225 (LT85_0834) pilT 943593..944636 (-) 1044 WP_038485669.1 type IV pilus twitching motility protein PilT Machinery gene
  LT85_RS04230 (LT85_0835) - 944855..945607 (+) 753 WP_038485672.1 YggS family pyridoxal phosphate-dependent enzyme -
  LT85_RS04235 (LT85_0836) proC 945604..946428 (+) 825 WP_038485675.1 pyrroline-5-carboxylate reductase -
  LT85_RS04240 (LT85_0837) - 946697..947017 (-) 321 WP_038485678.1 YqjD family protein -
  LT85_RS04245 (LT85_0838) ubiA 947115..947969 (-) 855 WP_038485681.1 4-hydroxybenzoate octaprenyltransferase -
  LT85_RS04250 (LT85_0839) - 948065..949012 (-) 948 WP_038485684.1 hydrogen peroxide-inducible genes activator -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38108.67 Da        Isoelectric Point: 6.6930

>NTDB_id=133190 LT85_RS04225 WP_038485669.1 943593..944636(-) (pilT) [Collimonas arenae strain Cal35]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHSMIYDIMNDGQRKAYEEHLECDFSFEIPGLAR
FRVNAFNQDRGAAAVLRTIPSTVLTLEQLNTPRIFAELSLKPRGLVLVTGPTGSGKSTTLAAMVNHVNENEYAHILTIED
PIEFVHQSNKCLINQREVGPHTHSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRAMLSESLQAVISQTLLKTKDGSGRVAAHEIMLGTPAIRNLIRESKIAQMYSAIQTGSNMGMQTLDSN
LTELVKRNVISLAAARAAAKTPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=133190 LT85_RS04225 WP_038485669.1 943593..944636(-) (pilT) [Collimonas arenae strain Cal35]
ATGGACATTTCCGAACTTCTGGCTTTTTCGGTAAAGAATAAGGCATCCGATTTGCATTTATCCGCAGGCTTGCCGCCGAT
GATCCGGGTTCATGGAGATGTGCGTCGCATCAACCTGCCGCCGCTCGAGCACAAAGACGTGCACTCGATGATCTATGACA
TCATGAACGATGGCCAGCGCAAAGCCTACGAAGAACACCTGGAATGCGATTTTTCGTTCGAGATTCCCGGCCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGGATCGCGGCGCCGCCGCCGTGTTGCGGACCATTCCATCCACCGTGCTGACCCTGGA
GCAACTCAACACGCCGCGCATTTTTGCCGAGCTGTCGCTCAAGCCGCGCGGCCTGGTGCTGGTCACCGGCCCTACCGGCT
CGGGTAAATCGACCACGCTGGCGGCGATGGTGAATCATGTCAATGAAAACGAATACGCACACATCCTGACCATTGAAGAC
CCGATCGAATTTGTCCATCAATCCAACAAATGCCTGATCAACCAGCGTGAAGTCGGCCCGCATACCCATTCGTTCAACAA
TGCGCTGCGTTCGGCCTTGCGTGAAGACCCGGACGTGATCCTGGTCGGCGAATTGCGTGACCTGGAAACCATCCGCCTCG
CGCTCACAGCAGCCGAAACCGGCCATCTGGTATTCGGTACCTTGCACACATCCTCCGCAGCAAAAACCATCGACCGTATC
GTCGACGTTTTCCCGGCGGAAGAAAAAGAAATGGTGCGCGCCATGTTGTCGGAATCGCTGCAGGCTGTGATCTCGCAAAC
CCTGTTGAAAACCAAGGATGGTTCGGGCCGCGTCGCCGCCCATGAAATCATGCTGGGTACGCCGGCGATCCGCAACCTGA
TCCGCGAAAGCAAGATCGCCCAGATGTATTCGGCGATCCAGACCGGCAGCAACATGGGCATGCAAACCCTGGACAGCAAT
CTGACCGAACTGGTCAAGCGCAACGTCATTTCACTGGCTGCTGCGCGCGCTGCGGCCAAGACGCCGGATAACTTCCCAGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A1F8J4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.971

99.424

0.775
  pilT Acinetobacter baumannii D1279779

77.971

99.424

0.775
  pilT Acinetobacter baumannii strain A118

77.971

99.424

0.775
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

75.221

97.695

0.735
  pilT Legionella pneumophila strain ERS1305867

75.221

97.695

0.735
  pilT Neisseria gonorrhoeae MS11

68.986

99.424

0.686
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.212

95.101

0.677
  pilT Vibrio cholerae strain A1552

71.212

95.101

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Pseudomonas stutzeri DSM 10701

40.58

99.424

0.403
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403
  pilU Vibrio cholerae strain A1552

39.42

99.424

0.392

Multiple sequence alignment