Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LT85_RS04220 Genome accession   NZ_CP009962
Coordinates   942406..943542 (-) Length   378 a.a.
NCBI ID   WP_038485666.1    Uniprot ID   A0A0A1F5J7
Organism   Collimonas arenae strain Cal35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 937406..948542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LT85_RS04200 (LT85_0828) glpD 937835..939367 (+) 1533 WP_052134637.1 glycerol-3-phosphate dehydrogenase -
  LT85_RS04205 (LT85_0830) glpK 939545..941041 (+) 1497 WP_038485657.1 glycerol kinase GlpK -
  LT85_RS04210 (LT85_0831) - 941132..941845 (+) 714 WP_038485660.1 MIP/aquaporin family protein -
  LT85_RS04215 (LT85_0832) - 941906..942409 (-) 504 WP_038485663.1 glutathione peroxidase -
  LT85_RS04220 (LT85_0833) pilU 942406..943542 (-) 1137 WP_038485666.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LT85_RS04225 (LT85_0834) pilT 943593..944636 (-) 1044 WP_038485669.1 type IV pilus twitching motility protein PilT Machinery gene
  LT85_RS04230 (LT85_0835) - 944855..945607 (+) 753 WP_038485672.1 YggS family pyridoxal phosphate-dependent enzyme -
  LT85_RS04235 (LT85_0836) proC 945604..946428 (+) 825 WP_038485675.1 pyrroline-5-carboxylate reductase -
  LT85_RS04240 (LT85_0837) - 946697..947017 (-) 321 WP_038485678.1 YqjD family protein -
  LT85_RS04245 (LT85_0838) ubiA 947115..947969 (-) 855 WP_038485681.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42026.40 Da        Isoelectric Point: 6.4213

>NTDB_id=133189 LT85_RS04220 WP_038485666.1 942406..943542(-) (pilU) [Collimonas arenae strain Cal35]
MERDQATKFMHDLLRLMVSKRGSDLFITADFPPAFKIDGKITPVSNQALTAMHTLELARAIMSDKQAADFEETKECNFAI
SPGGLGRFRASAFIQQGRVGMVLRTITTDIPKFEDLGLPATLKDVAMTKRGLVIMVGATGSGKSTTLAAMIGYRNENSYG
HIITIEDPVEYIHPHKNCIVTQREVGVDTENWGVALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCLATLHANSS
NQALDRIINFFPEERRPQLLMDLSLNLKGIVSQRLVPLKTVKGRAVAVEILLNSPLISDLIFKGDVHEIKEIMKKSRELG
MQTFDQALFDLYEDDKISYEDALRNADSVNDLRLSIKLRGKDAKDRDLSAGTEHLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=133189 LT85_RS04220 WP_038485666.1 942406..943542(-) (pilU) [Collimonas arenae strain Cal35]
ATGGAACGCGATCAAGCCACCAAATTCATGCACGACCTGCTGCGCCTGATGGTCAGCAAACGCGGTTCCGACCTGTTCAT
TACCGCAGACTTCCCGCCGGCATTCAAGATCGACGGCAAGATCACGCCGGTCTCGAATCAGGCCCTGACCGCGATGCATA
CGCTGGAACTGGCGCGCGCAATCATGAGCGACAAACAGGCTGCCGATTTTGAAGAAACCAAGGAATGCAATTTCGCCATC
AGCCCAGGCGGGCTGGGACGCTTCCGCGCTTCGGCATTTATCCAGCAGGGCCGGGTCGGCATGGTATTGCGTACGATTAC
CACCGATATTCCGAAATTCGAAGATCTAGGCCTGCCGGCGACGCTCAAGGACGTGGCGATGACCAAGCGCGGCCTGGTCA
TCATGGTCGGCGCGACCGGTTCCGGAAAATCGACCACCCTGGCGGCGATGATCGGCTACCGCAATGAGAACAGCTACGGC
CATATCATCACGATTGAAGATCCGGTGGAATATATCCATCCGCACAAGAACTGCATCGTGACCCAGCGCGAAGTCGGCGT
CGATACCGAAAACTGGGGCGTGGCCCTGAAGAACACCTTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCG
ACCGCGAAACCATGGACTACGCCATTGCCTTCGCCGAAACCGGCCACTTGTGCCTGGCTACCCTGCATGCCAACAGCTCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCAGAAGAACGCCGGCCGCAGTTGCTGATGGATTTGTCGCTTAACCT
GAAAGGCATTGTCTCGCAACGGCTGGTGCCGCTGAAAACGGTCAAGGGCCGTGCGGTAGCGGTTGAAATCCTGCTCAATT
CGCCATTGATTTCCGACCTGATTTTCAAGGGCGACGTCCATGAAATCAAGGAAATCATGAAGAAGTCGCGCGAGCTCGGC
ATGCAGACTTTCGACCAGGCCCTGTTTGATTTGTACGAAGACGATAAAATCAGCTACGAAGACGCTTTGCGCAACGCCGA
TTCGGTCAATGACCTGCGGCTTTCCATCAAACTGCGCGGCAAGGATGCAAAAGACCGCGACCTCTCTGCCGGCACTGAAC
ATCTGGGGATTGTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A1F5J7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.474

91.534

0.608
  pilU Acinetobacter baylyi ADP1

59.714

92.593

0.553
  pilU Vibrio cholerae strain A1552

57.637

91.799

0.529
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.881

88.624

0.389
  pilT Pseudomonas aeruginosa PAK

41.194

88.624

0.365
  pilT Acinetobacter baumannii D1279779

41.39

87.566

0.362
  pilT Acinetobacter baumannii strain A118

41.39

87.566

0.362
  pilT Acinetobacter nosocomialis M2

41.39

87.566

0.362

Multiple sequence alignment