Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IX92_RS13305 Genome accession   NZ_CP009617
Coordinates   2888551..2889588 (-) Length   345 a.a.
NCBI ID   WP_043009311.1    Uniprot ID   A0AAN0VXY8
Organism   Vibrio coralliilyticus strain RE98     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2883551..2894588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IX92_RS13275 (IX92_13275) - 2883755..2884459 (+) 705 WP_038159793.1 endonuclease -
  IX92_RS13280 (IX92_13280) rsmE 2884568..2885299 (+) 732 WP_043009307.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IX92_RS13285 (IX92_13285) gshB 2885313..2886263 (+) 951 WP_043009308.1 glutathione synthase -
  IX92_RS13290 (IX92_13290) - 2886320..2886883 (+) 564 WP_043009309.1 YqgE/AlgH family protein -
  IX92_RS13295 (IX92_13295) ruvX 2886901..2887323 (+) 423 WP_029236265.1 Holliday junction resolvase RuvX -
  IX92_RS13300 (IX92_13300) pilU 2887433..2888539 (-) 1107 WP_043009310.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IX92_RS13305 (IX92_13305) pilT 2888551..2889588 (-) 1038 WP_043009311.1 type IV pilus twitching motility protein PilT Machinery gene
  IX92_RS13310 (IX92_13310) - 2889613..2890329 (+) 717 WP_043009313.1 YggS family pyridoxal phosphate-dependent enzyme -
  IX92_RS13315 (IX92_13315) proC 2890357..2891175 (+) 819 WP_043009315.1 pyrroline-5-carboxylate reductase -
  IX92_RS13320 (IX92_13320) - 2891214..2891771 (+) 558 WP_006958264.1 YggT family protein -
  IX92_RS13325 (IX92_13325) yggU 2891771..2892061 (+) 291 WP_038509522.1 DUF167 family protein YggU -
  IX92_RS13330 (IX92_13330) - 2892260..2892691 (+) 432 WP_043009317.1 DUF4426 domain-containing protein -
  IX92_RS13335 (IX92_13335) - 2892731..2893333 (+) 603 WP_019277254.1 XTP/dITP diphosphatase -
  IX92_RS13340 (IX92_13340) hemW 2893333..2894511 (+) 1179 WP_043009320.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38098.45 Da        Isoelectric Point: 6.1084

>NTDB_id=130786 IX92_RS13305 WP_043009311.1 2888551..2889588(-) (pilT) [Vibrio coralliilyticus strain RE98]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGVPAFNHSEVHRLVFEIMNDAQRSEFEERLEVDFSFALPDVGR
FRVNAFNQSRGCAAVFRTIPTDIPTLEQLEAPSIFENIANMEKGLILVTGPTGSGKSTTLAAMVDHINRNHNKHILTIED
PIEFVHTNNKCLINQREVHNDTHSFNNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKGMVRSMLSESLRAVIAQKLLKRIGGGRTACHEIMMATPAIRNLIREDKVAQMQSVIQTGAAHGMQTMEQHS
KQLIAQGLVDAEEVSKKIEVEQGLF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=130786 IX92_RS13305 WP_043009311.1 2888551..2889588(-) (pilT) [Vibrio coralliilyticus strain RE98]
ATGGATATCGCTGAATTACTGGATTTTAGTGTAAAGCATAATGCTTCAGATCTACATCTTTCTGCTGGTGTATCTCCCAT
GGTACGCATTGATGGTGAAGTAAGAAAGCTTGGCGTACCAGCGTTCAACCATTCCGAAGTGCATCGTTTGGTGTTTGAAA
TTATGAACGATGCTCAGCGTAGCGAATTTGAAGAACGTCTTGAAGTCGACTTTTCTTTTGCACTTCCGGACGTAGGCCGC
TTTAGGGTCAACGCTTTTAACCAATCTCGTGGTTGTGCGGCCGTATTTCGTACCATTCCAACCGATATCCCGACTTTAGA
GCAGCTAGAAGCGCCGTCGATCTTTGAAAATATCGCCAATATGGAGAAAGGACTAATCTTGGTCACTGGGCCAACAGGCT
CAGGTAAATCGACTACTTTGGCGGCAATGGTGGATCATATTAATCGCAACCACAATAAGCATATCCTGACTATTGAAGAT
CCGATTGAGTTTGTGCACACCAATAACAAATGTCTGATCAACCAGCGTGAAGTTCACAATGATACCCATAGTTTTAATAA
CGCGCTGCGCAGTGCATTGCGTGAAGATCCCGATGTCATTTTAGTCGGTGAGCTACGTGATCAGGAAACGATCAGTCTAG
CGTTGACCGCGGCTGAAACCGGCCACTTGGTATTTGGTACTTTGCACACCAGTTCGGCGGCGAAAACTATCGACCGTATC
ATTGATGTTTTCCCGGGTAGCGACAAAGGCATGGTGCGTTCCATGCTCTCTGAGTCTCTGCGTGCGGTGATTGCGCAGAA
ATTACTCAAGCGCATTGGCGGCGGGAGAACCGCTTGTCATGAAATCATGATGGCAACGCCTGCGATTCGTAACTTGATCC
GCGAAGACAAAGTCGCGCAAATGCAGTCTGTGATTCAGACTGGTGCGGCGCACGGTATGCAAACCATGGAGCAGCACTCA
AAACAGTTGATTGCTCAAGGTCTGGTTGATGCAGAAGAAGTGTCGAAAAAAATCGAAGTTGAGCAAGGTCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

86.957

100

0.87
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.957

100

0.87
  pilT Acinetobacter baylyi ADP1

68.421

99.13

0.678
  pilT Acinetobacter baumannii D1279779

69.578

96.232

0.67
  pilT Acinetobacter baumannii strain A118

69.578

96.232

0.67
  pilT Acinetobacter nosocomialis M2

69.277

96.232

0.667
  pilT Pseudomonas stutzeri DSM 10701

66.766

97.681

0.652
  pilT Pseudomonas aeruginosa PAK

65.497

99.13

0.649
  pilT Legionella pneumophila strain Lp02

66.869

95.362

0.638
  pilT Legionella pneumophila strain ERS1305867

66.869

95.362

0.638
  pilT Neisseria meningitidis 8013

64.497

97.971

0.632
  pilT Neisseria gonorrhoeae MS11

63.235

98.551

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.818

95.652

0.496
  pilU Vibrio cholerae strain A1552

42.154

94.203

0.397
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.812

0.391
  pilU Acinetobacter baylyi ADP1

40.938

92.754

0.38

Multiple sequence alignment