Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IX92_RS13300 Genome accession   NZ_CP009617
Coordinates   2887433..2888539 (-) Length   368 a.a.
NCBI ID   WP_043009310.1    Uniprot ID   A0AAN0SCM3
Organism   Vibrio coralliilyticus strain RE98     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2882433..2893539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IX92_RS13270 (IX92_13270) - 2883178..2883675 (+) 498 WP_043009675.1 SprT family zinc-dependent metalloprotease -
  IX92_RS13275 (IX92_13275) - 2883755..2884459 (+) 705 WP_038159793.1 endonuclease -
  IX92_RS13280 (IX92_13280) rsmE 2884568..2885299 (+) 732 WP_043009307.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IX92_RS13285 (IX92_13285) gshB 2885313..2886263 (+) 951 WP_043009308.1 glutathione synthase -
  IX92_RS13290 (IX92_13290) - 2886320..2886883 (+) 564 WP_043009309.1 YqgE/AlgH family protein -
  IX92_RS13295 (IX92_13295) ruvX 2886901..2887323 (+) 423 WP_029236265.1 Holliday junction resolvase RuvX -
  IX92_RS13300 (IX92_13300) pilU 2887433..2888539 (-) 1107 WP_043009310.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IX92_RS13305 (IX92_13305) pilT 2888551..2889588 (-) 1038 WP_043009311.1 type IV pilus twitching motility protein PilT Machinery gene
  IX92_RS13310 (IX92_13310) - 2889613..2890329 (+) 717 WP_043009313.1 YggS family pyridoxal phosphate-dependent enzyme -
  IX92_RS13315 (IX92_13315) proC 2890357..2891175 (+) 819 WP_043009315.1 pyrroline-5-carboxylate reductase -
  IX92_RS13320 (IX92_13320) - 2891214..2891771 (+) 558 WP_006958264.1 YggT family protein -
  IX92_RS13325 (IX92_13325) yggU 2891771..2892061 (+) 291 WP_038509522.1 DUF167 family protein YggU -
  IX92_RS13330 (IX92_13330) - 2892260..2892691 (+) 432 WP_043009317.1 DUF4426 domain-containing protein -
  IX92_RS13335 (IX92_13335) - 2892731..2893333 (+) 603 WP_019277254.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41233.89 Da        Isoelectric Point: 5.3077

>NTDB_id=130785 IX92_RS13300 WP_043009310.1 2887433..2888539(-) (pilU) [Vibrio coralliilyticus strain RE98]
MDINPYLQGMLAEKASDLYITVGAPILYRVDGELKPHGESLSEAEVLTLLHSTMDEERQNDYSHSREANFAVVRDFGRFR
VSAFYQRELPGAVIRRIETTIPTFDDLKLPETLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTERSGHILTVEDPI
EFVHEHQKCIVTQREVGLDTESYEIALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMAKSQEIGMQTFDQALY
QLVVNDKITEEDALHSADSANDLRIMLKTQRGESFGGGALADVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=130785 IX92_RS13300 WP_043009310.1 2887433..2888539(-) (pilU) [Vibrio coralliilyticus strain RE98]
ATGGATATCAATCCTTATCTGCAAGGCATGCTGGCTGAGAAAGCGTCAGACTTATACATCACTGTTGGTGCGCCGATACT
TTATCGTGTTGATGGTGAGTTGAAGCCGCATGGAGAGAGCTTATCGGAAGCCGAAGTGCTGACGCTACTCCACAGCACCA
TGGATGAAGAGCGCCAGAATGACTATAGCCACAGTCGAGAAGCGAACTTTGCCGTAGTGCGGGATTTTGGTCGTTTTCGT
GTGTCCGCTTTCTACCAGCGAGAATTACCCGGGGCAGTGATTCGTAGAATCGAAACCACCATACCCACATTTGATGACTT
AAAACTGCCAGAAACTCTGAAAGATCTTTCCATCGCCAAGCGCGGCTTGGTGTTAGTTGTGGGTGCCACAGGTTCCGGTA
AATCCACAACAATGGCAGCGATGACGGGCTATCGAAATACTGAGCGCAGCGGCCATATTCTGACAGTTGAAGACCCGATA
GAGTTTGTCCACGAGCATCAGAAGTGCATTGTCACGCAACGTGAGGTTGGCTTAGACACGGAAAGTTATGAAATCGCACT
GAAGAACTCTTTGCGTCAGGCTCCTGATATGATTTTGATCGGTGAGATCCGCTCGCGAGAGACCATGGAATACGCGATGA
CATTCGCCGAAACGGGGCATCTGTGTATGGCGACGTTGCATGCTAATAATGCTAACCAAGCTTTGGAAAGGATCCTGCAC
CTTGTACCGAAAGAGCAAAAAGAGCAGTTCCTGTTTGATCTTTCAATGAACCTCAAAGGTGTTATCGGTCAGCAGTTGAT
TCGAGATAAAAACGGCCAAGGTCGTCATGGCGTTTTTGAAATTTTACTCAACAGCCCACGAGTATCGGATCTCATTCGAC
GCGGTGATCTGCATGAACTCAAATCAACCATGGCTAAATCTCAGGAAATTGGTATGCAGACGTTTGATCAGGCGCTTTAC
CAACTGGTGGTGAATGACAAAATTACTGAAGAAGATGCGCTGCACAGTGCAGATTCAGCCAATGATCTGCGTATCATGCT
TAAAACTCAACGAGGTGAAAGCTTCGGTGGTGGTGCGCTGGCAGATGTGAAGATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.337

100

0.823
  pilU Pseudomonas stutzeri DSM 10701

57.143

95.109

0.543
  pilU Acinetobacter baylyi ADP1

53.276

95.38

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.248

92.12

0.408
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Acinetobacter baylyi ADP1

41.935

92.663

0.389
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.118

92.12

0.37
  pilT Neisseria meningitidis 8013

39.13

93.75

0.367
  pilT Neisseria gonorrhoeae MS11

39.13

93.75

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.231

88.315

0.364
  pilT Vibrio cholerae strain A1552

41.231

88.315

0.364

Multiple sequence alignment