Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CH92_RS19375 Genome accession   NZ_CP007441
Coordinates   4266756..4267790 (-) Length   344 a.a.
NCBI ID   WP_025243414.1    Uniprot ID   W8R3M1
Organism   Stutzerimonas stutzeri strain 28a24     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4261756..4272790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH92_RS19355 (CH92_19780) - 4262408..4262806 (-) 399 WP_025243410.1 TM2 domain-containing protein -
  CH92_RS19360 (CH92_19785) - 4262943..4264154 (+) 1212 WP_025243411.1 putative DNA modification/repair radical SAM protein -
  CH92_RS22455 (CH92_19790) - 4264154..4265533 (+) 1380 WP_025243412.1 TIGR03915 family putative DNA repair protein -
  CH92_RS19370 (CH92_19795) pilU 4265534..4266679 (-) 1146 WP_025243413.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CH92_RS19375 (CH92_19800) pilT 4266756..4267790 (-) 1035 WP_025243414.1 type IV pilus twitching motility protein PilT Machinery gene
  CH92_RS19380 (CH92_19805) - 4267857..4268570 (+) 714 WP_025243415.1 YggS family pyridoxal phosphate-dependent enzyme -
  CH92_RS19385 (CH92_19810) proC 4268583..4269410 (+) 828 WP_025243416.1 pyrroline-5-carboxylate reductase -
  CH92_RS19390 (CH92_19815) - 4269687..4270277 (+) 591 WP_025243417.1 YggT family protein -
  CH92_RS19395 (CH92_19820) - 4270398..4272359 (+) 1962 WP_025243418.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37914.68 Da        Isoelectric Point: 6.8360

>NTDB_id=119368 CH92_RS19375 WP_025243414.1 4266756..4267790(-) (pilT) [Stutzerimonas stutzeri strain 28a24]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDACL
KGLLAKGLISRDAAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=119368 CH92_RS19375 WP_025243414.1 4266756..4267790(-) (pilT) [Stutzerimonas stutzeri strain 28a24]
ATGGATATCACAGAACTGCTGGCCTTCAGCGCCAAGCAGGGTGCGTCTGACTTGCACCTGTCTTCCGGCCTGCCGCCGAT
GATCCGCGTTGACGGCGATGTGCGCCGGATCAATCTGCCGGCGATGGACCACAAGCAGGTGCACGCACTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAATTTCTCGAGACCGACTTTTCGTTCGAAGTGCCCGGCGTGGCACGC
TTCCGGGTCAACGCGTTCAACCAGAATCGCGGTGCCGGTGCGGTGTTCCGGACCATTCCGTCGAAAGTGCTGACCATGGA
AGATCTCGGTATGGGCGAGGTCTTCCGCAAGATTACCGATGTACCTCGAGGGCTGGTGCTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCAACCACCCTGGCGGCGATGCTCGACTATCTGAACAACACCAAATATCACCACATCCTCACCATCGAAGAC
CCGATCGAGTTCGTCCACGAGTCGAAAAAGTGTCTGGTCAACCAACGTGAAGTGCACCGCGACACACTGGGCTTCTCCGA
AGCCTTGCGCTCGGCGCTTCGTGAAGACCCGGACATCATCCTCGTCGGCGAGATGCGCGACCTGGAAACCATCCGGCTGG
CGCTGACCGCTGCGGAGACTGGGCACTTGGTGTTCGGTACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGGTG
GTCGACGTATTCCCGGCTGAAGAAAAGTCGATGGTCCGCTCGATGTTGTCCGAATCTCTGCAGGCGGTGATTTCCCAGAC
CCTGCTGAAGAAGGTCGGTGGTGGTCGGGTGGCCGCGCACGAAATCATGATCGGCACGCCTGCGATCCGAAACCTGATTC
GAGAGGACAAGGTGGCGCAAATGTATTCCTCGATCCAGACCGGCGGTTCGCTGGGCATGCAGACGCTCGATGCCTGTCTC
AAGGGACTGCTGGCCAAGGGACTGATTTCGCGCGACGCGGCCAAGGAAAAGGCCAAGCAGCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8R3M1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

99.419

100

0.994
  pilT Pseudomonas aeruginosa PAK

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

83.43

100

0.834
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment