Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CH92_RS19370 Genome accession   NZ_CP007441
Coordinates   4265534..4266679 (-) Length   381 a.a.
NCBI ID   WP_025243413.1    Uniprot ID   W8RBX5
Organism   Stutzerimonas stutzeri strain 28a24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4260534..4271679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH92_RS19350 (CH92_19775) - 4261083..4262354 (+) 1272 WP_038623181.1 dihydroorotase -
  CH92_RS19355 (CH92_19780) - 4262408..4262806 (-) 399 WP_025243410.1 TM2 domain-containing protein -
  CH92_RS19360 (CH92_19785) - 4262943..4264154 (+) 1212 WP_025243411.1 putative DNA modification/repair radical SAM protein -
  CH92_RS22455 (CH92_19790) - 4264154..4265533 (+) 1380 WP_025243412.1 TIGR03915 family putative DNA repair protein -
  CH92_RS19370 (CH92_19795) pilU 4265534..4266679 (-) 1146 WP_025243413.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CH92_RS19375 (CH92_19800) pilT 4266756..4267790 (-) 1035 WP_025243414.1 type IV pilus twitching motility protein PilT Machinery gene
  CH92_RS19380 (CH92_19805) - 4267857..4268570 (+) 714 WP_025243415.1 YggS family pyridoxal phosphate-dependent enzyme -
  CH92_RS19385 (CH92_19810) proC 4268583..4269410 (+) 828 WP_025243416.1 pyrroline-5-carboxylate reductase -
  CH92_RS19390 (CH92_19815) - 4269687..4270277 (+) 591 WP_025243417.1 YggT family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42420.64 Da        Isoelectric Point: 6.5054

>NTDB_id=119367 CH92_RS19370 WP_025243413.1 4265534..4266679(-) (pilU) [Stutzerimonas stutzeri strain 28a24]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKILPVNKTPMSPEMTRETVHAVMNEQQRREFAENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTLEDLKLPDVLKKLAMTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTDSFDVALKNTLRQAPDVILIGEVRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLISTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTDLGMQTFDQA
LYNLYVQGEITYEDALLHADSSNDLRLMIKLGSETDGEHLTSVSQGLTLEVSDDDPGRSFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=119367 CH92_RS19370 WP_025243413.1 4265534..4266679(-) (pilU) [Stutzerimonas stutzeri strain 28a24]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTTGAAAAGGGCGGCTCCGATCTGTTCATTACTGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCCTGCCGGTGAACAAAACGCCGATGTCGCCGGAGATGACTCGCGAAACCGTGCATGCCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCGCGGAAAACCACGAATGCAACTTTGCCATCAGTGCGCGTGGCATTGGTCGT
TTTCGTGTCAGCGCCTTCTATCAGCGCAACCTCGCCGGCATGGTGCTACGCCGTATCGAGACCAATATCCCGACGCTCGA
AGATCTGAAACTGCCGGACGTGCTGAAAAAACTGGCGATGACTAAGCGCGGTCTGGTGCTGTTCGTCGGGGCCACCGGCA
CCGGCAAGTCCACTTCGCTGGCGGCGATGATCGGCTATCGCAACAAGAACTCCAGCGGCCATATCATTTCGATTGAAGAC
CCCATCGAGTTCATTCATCAGCATCAAAGCTGCATCGTTACTCAGCGTGAAGTCGGTATCGACACCGACTCCTTCGATGT
GGCGCTGAAAAACACCCTGCGTCAGGCGCCGGATGTAATTCTTATCGGCGAGGTGCGGACGCGCGAAACCATGGACTACG
CCGTGGCGTTCGCCGAAACCGGCCACCTGTGCCTTGCGACCTTGCACGCCAACAACGCTAACCAGGCGCTGGATCGAATC
ATCAACTTCTTCCCGGCTGATCGTCAGCAGCAGGTCTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGATTTCCACGCCCGACGGTAAAGGCCGGCGGGCGGTCATCGAAGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAAGTGCACGAGCTCAAATCACTGATGAAGCGCTCTACTGACCTCGGCATGCAGACGTTCGATCAGGCG
CTCTACAACCTCTACGTGCAGGGCGAAATCACCTACGAAGACGCACTGCTGCATGCCGATTCGTCCAACGACCTACGCCT
GATGATCAAGTTGGGCTCGGAAACCGATGGCGAACATCTGACCTCCGTATCCCAGGGGCTCACTTTGGAAGTCAGCGATG
ACGATCCGGGGCGTAGCTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8RBX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

96.85

100

0.969
  pilU Acinetobacter baylyi ADP1

65.826

93.701

0.617
  pilU Vibrio cholerae strain A1552

59.143

91.864

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.658

88.976

0.388
  pilT Neisseria meningitidis 8013

40.87

90.551

0.37
  pilT Legionella pneumophila strain Lp02

42.09

87.927

0.37
  pilT Legionella pneumophila strain ERS1305867

42.09

87.927

0.37
  pilT Neisseria gonorrhoeae MS11

40.87

90.551

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.42

88.714

0.367
  pilT Vibrio cholerae strain A1552

41.42

88.714

0.367
  pilT Pseudomonas aeruginosa PAK

41.493

87.927

0.365
  pilT Pseudomonas stutzeri DSM 10701

41.194

87.927

0.362

Multiple sequence alignment