Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACOQOE_RS02380 Genome accession   NZ_OZ245004
Coordinates   502189..503223 (+) Length   344 a.a.
NCBI ID   WP_067866669.1    Uniprot ID   -
Organism   MAG: Neptuniibacter marinus isolate 5ffc281b-0633-46d3-a470-07ce90f1f764     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 497189..508223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOQOE_RS02345 - 497205..497654 (-) 450 WP_067866682.1 DUF4426 domain-containing protein -
  ACOQOE_RS02350 metW 497666..498256 (-) 591 WP_420554819.1 methionine biosynthesis protein MetW -
  ACOQOE_RS02355 metX 498256..499425 (-) 1170 WP_420554820.1 homoserine O-succinyltransferase MetX -
  ACOQOE_RS02360 - 499565..499855 (-) 291 WP_420554821.1 DUF167 domain-containing protein -
  ACOQOE_RS02365 - 499857..500402 (-) 546 WP_067866677.1 YggT family protein -
  ACOQOE_RS02370 proC 500524..501351 (-) 828 WP_420554822.1 pyrroline-5-carboxylate reductase -
  ACOQOE_RS02375 - 501413..502102 (-) 690 WP_067866785.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACOQOE_RS02380 pilT 502189..503223 (+) 1035 WP_067866669.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOQOE_RS02385 pilU 503257..504420 (+) 1164 WP_067866668.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOQOE_RS02390 - 504500..505777 (-) 1278 WP_420554823.1 dihydroorotase -
  ACOQOE_RS02395 - 505783..506787 (-) 1005 WP_067866663.1 aspartate carbamoyltransferase catalytic subunit -
  ACOQOE_RS02400 pyrR 506797..507312 (-) 516 WP_420554824.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACOQOE_RS02405 ruvX 507293..507769 (-) 477 WP_067866659.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38192.84 Da        Isoelectric Point: 6.6680

>NTDB_id=1171212 ACOQOE_RS02380 WP_067866669.1 502189..503223(+) (pilT) [MAG: Neptuniibacter marinus isolate 5ffc281b-0633-46d3-a470-07ce90f1f764]
MDITELLSFTVQQGASDLHISAGMPPIIRVDGEVRRIKLPSLDHKQVHTLIYDIMNDKQRKEYEDTLETDFSFEVPGLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDDLDMGKVFRDLSELPRGLVLVTGPTGSGKSTTLAAMIDYVNDTRADHILTIED
PIEFVHESKKCLVNQREVHRDTHSFANALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKDMVRSMLSESLMGVISQTLLKKPKGGRVAAHEIMIGTPAIRNLIREAKVAQMYSAIQTGASFGMKTLDQSL
QELVQKGMISRETARERAANPQQF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1171212 ACOQOE_RS02380 WP_067866669.1 502189..503223(+) (pilT) [MAG: Neptuniibacter marinus isolate 5ffc281b-0633-46d3-a470-07ce90f1f764]
ATGGATATTACCGAACTTTTATCATTTACCGTACAGCAAGGCGCGTCTGACCTACATATTTCTGCTGGTATGCCTCCCAT
TATTCGTGTTGATGGCGAGGTTCGTCGTATTAAACTTCCCTCTTTAGATCATAAACAGGTGCATACGCTGATTTATGACA
TTATGAACGATAAACAGCGTAAAGAGTATGAAGATACGCTTGAAACTGATTTCTCGTTTGAGGTGCCAGGTCTTGCACGT
TTTCGTGTTAATGCGTTTAACCAAAACCGTGGTGCGGGCGCTGTTTTTCGTACAATCCCGAGTAAAGTATTGACGCTAGA
TGACCTCGATATGGGGAAAGTTTTCAGAGACCTATCTGAGCTTCCAAGGGGACTTGTACTTGTGACTGGGCCCACAGGTT
CGGGTAAGAGTACAACCTTAGCGGCAATGATTGATTATGTGAACGACACCCGCGCCGATCATATTCTTACTATTGAAGAT
CCCATCGAGTTTGTGCACGAAAGTAAGAAATGTTTAGTCAACCAGCGAGAAGTGCATCGAGATACCCATAGTTTCGCAAA
TGCATTGCGCTCAGCGTTACGTGAAGATCCGGATATTATTCTTGTCGGTGAGATGCGAGATTTAGAAACTATTCGTCTTG
CTTTAACTGCGGCAGAAACGGGGCATCTCGTCTTTGGAACACTACATACCACCTCGGCGGCTAAAACAATTGACCGTGTG
ATCGATGTTTTCCCAGCAGCAGAGAAAGATATGGTCCGCTCAATGTTATCTGAGTCGCTGATGGGCGTTATCTCCCAAAC
GCTATTGAAAAAACCTAAAGGGGGGCGTGTTGCTGCCCATGAAATTATGATTGGTACGCCTGCCATTCGTAACTTAATTC
GTGAGGCTAAAGTTGCGCAGATGTATTCAGCGATACAAACGGGTGCTTCGTTTGGTATGAAGACATTGGATCAATCTCTG
CAAGAATTGGTTCAAAAAGGTATGATATCCCGAGAAACGGCCCGAGAACGTGCAGCAAATCCGCAGCAGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

82.267

100

0.823
  pilT Pseudomonas stutzeri DSM 10701

81.395

100

0.814
  pilT Acinetobacter baumannii D1279779

81.105

100

0.811
  pilT Acinetobacter baumannii strain A118

81.105

100

0.811
  pilT Acinetobacter nosocomialis M2

81.105

100

0.811
  pilT Acinetobacter baylyi ADP1

80.233

100

0.802
  pilT Legionella pneumophila strain Lp02

72.674

100

0.727
  pilT Legionella pneumophila strain ERS1305867

72.674

100

0.727
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.656

97.965

0.663
  pilT Vibrio cholerae strain A1552

67.656

97.965

0.663
  pilT Neisseria meningitidis 8013

66.472

99.709

0.663
  pilT Neisseria gonorrhoeae MS11

66.181

99.709

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.5
  pilU Vibrio cholerae strain A1552

42.09

97.384

0.41
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401

Multiple sequence alignment