Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACOQOE_RS02385 Genome accession   NZ_OZ245004
Coordinates   503257..504420 (+) Length   387 a.a.
NCBI ID   WP_067866668.1    Uniprot ID   -
Organism   MAG: Neptuniibacter marinus isolate 5ffc281b-0633-46d3-a470-07ce90f1f764     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 498257..509420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOQOE_RS02360 - 499565..499855 (-) 291 WP_420554821.1 DUF167 domain-containing protein -
  ACOQOE_RS02365 - 499857..500402 (-) 546 WP_067866677.1 YggT family protein -
  ACOQOE_RS02370 proC 500524..501351 (-) 828 WP_420554822.1 pyrroline-5-carboxylate reductase -
  ACOQOE_RS02375 - 501413..502102 (-) 690 WP_067866785.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACOQOE_RS02380 pilT 502189..503223 (+) 1035 WP_067866669.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOQOE_RS02385 pilU 503257..504420 (+) 1164 WP_067866668.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOQOE_RS02390 - 504500..505777 (-) 1278 WP_420554823.1 dihydroorotase -
  ACOQOE_RS02395 - 505783..506787 (-) 1005 WP_067866663.1 aspartate carbamoyltransferase catalytic subunit -
  ACOQOE_RS02400 pyrR 506797..507312 (-) 516 WP_420554824.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACOQOE_RS02405 ruvX 507293..507769 (-) 477 WP_067866659.1 Holliday junction resolvase RuvX -
  ACOQOE_RS02410 - 507826..508398 (-) 573 WP_067866657.1 YqgE/AlgH family protein -
  ACOQOE_RS02415 - 508434..509381 (-) 948 WP_067866654.1 energy transducer TonB -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43104.07 Da        Isoelectric Point: 6.1117

>NTDB_id=1171213 ACOQOE_RS02385 WP_067866668.1 503257..504420(+) (pilU) [MAG: Neptuniibacter marinus isolate 5ffc281b-0633-46d3-a470-07ce90f1f764]
MDFTQLLKVMVERDASDLFVTAGARPTIKVDGTLKPLTKDTLKPSQARALVYSTMNDKQLAEFEGSHECNFAISAPGLGR
FRVSAFFQRNSAGMVLRKINNHIPSLEELNLPPILRDLSMTKRGLILFVGGTSTGKSTSLASMIDYRNTNHRGHIITIED
PIEYIHEHKQSIITQREVGIDTESFDTALKNTLRQAPDVILMGEIRTRETMQHGIVFAETGHLTLATLHANNANQALDRV
ISFFPAEHHDQLWMDLSLNLKAIIAQQLLPTKDGKGRRAAIEVLINTPLIQDLIRKGEVHEIKEVVKKSTNLGMQTFDQA
LFALYKDGEITYDVALAHADSPNDLRLMIKLNSDTNPGLGVDDGQSFHLQEEEDYLHNEGDANVSGM

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=1171213 ACOQOE_RS02385 WP_067866668.1 503257..504420(+) (pilU) [MAG: Neptuniibacter marinus isolate 5ffc281b-0633-46d3-a470-07ce90f1f764]
GTGGATTTTACGCAATTACTGAAAGTAATGGTAGAAAGGGATGCTTCGGATCTCTTCGTGACTGCGGGTGCGCGTCCGAC
GATAAAGGTCGATGGTACGTTAAAGCCGTTAACTAAAGATACGTTAAAGCCGTCTCAGGCACGGGCGTTAGTCTATAGTA
CGATGAACGACAAACAGCTGGCCGAGTTTGAAGGTTCGCATGAGTGTAACTTTGCGATCAGTGCACCGGGTTTAGGTCGT
TTCCGTGTGTCAGCCTTTTTTCAGCGAAACTCTGCCGGTATGGTCCTTAGGAAGATTAATAATCATATCCCCTCTTTAGA
AGAGCTAAATCTCCCTCCTATTCTCCGTGATCTTTCCATGACAAAGCGCGGTTTGATTCTGTTTGTGGGGGGGACCTCAA
CCGGTAAATCTACTTCCCTAGCTTCAATGATTGATTACCGTAATACCAATCATCGCGGACATATCATTACGATCGAAGAC
CCGATTGAATATATCCATGAGCATAAGCAAAGTATTATTACTCAGCGTGAAGTGGGTATAGATACAGAATCTTTTGATAC
TGCGTTGAAAAATACGCTGCGTCAGGCTCCTGATGTCATTTTGATGGGGGAAATTCGTACGCGAGAAACGATGCAGCATG
GTATTGTATTTGCTGAAACAGGTCATCTTACGTTGGCCACGCTACATGCGAATAATGCCAACCAAGCGTTAGATCGAGTG
ATTAGTTTCTTTCCAGCAGAACACCATGATCAGTTGTGGATGGATCTATCATTGAACCTGAAAGCGATTATAGCTCAGCA
ACTCCTACCGACTAAGGATGGTAAGGGACGTCGAGCCGCGATTGAGGTCTTGATTAACACACCTTTGATTCAAGACCTTA
TCCGTAAAGGTGAAGTCCATGAGATAAAAGAGGTGGTGAAAAAATCTACAAACTTGGGGATGCAGACGTTTGATCAGGCT
CTTTTTGCGCTCTATAAAGATGGTGAAATTACTTATGACGTAGCTTTGGCCCATGCAGACTCACCTAATGATCTTCGCTT
GATGATTAAACTCAATTCAGATACAAATCCGGGTTTAGGTGTGGATGATGGGCAGTCTTTCCATCTTCAGGAAGAGGAAG
ATTATCTTCATAATGAAGGTGATGCTAACGTCAGCGGGATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.82

91.99

0.633
  pilU Acinetobacter baylyi ADP1

61.389

93.023

0.571
  pilU Vibrio cholerae strain A1552

54.167

93.023

0.504
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.333

93.023

0.403
  pilT Acinetobacter baylyi ADP1

44.444

88.372

0.393
  pilT Acinetobacter nosocomialis M2

43.275

88.372

0.382
  pilT Acinetobacter baumannii D1279779

43.275

88.372

0.382
  pilT Acinetobacter baumannii strain A118

43.275

88.372

0.382
  pilT Pseudomonas stutzeri DSM 10701

42.609

89.147

0.38
  pilT Pseudomonas aeruginosa PAK

42.609

89.147

0.38

Multiple sequence alignment