Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACOQN4_RS05315 Genome accession   NZ_OZ244735
Coordinates   1037503..1038540 (-) Length   345 a.a.
NCBI ID   WP_420551090.1    Uniprot ID   -
Organism   MAG: Litorivicinus lipolyticus isolate cfb32c0c-0c5a-4fc9-8b22-2b72eeb7acf8     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1032503..1043540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOQN4_RS05285 - 1032644..1033198 (+) 555 WP_420551087.1 YqgE/AlgH family protein -
  ACOQN4_RS05290 ruvX 1033195..1033608 (+) 414 WP_153712743.1 Holliday junction resolvase RuvX -
  ACOQN4_RS05295 - 1033652..1034620 (+) 969 WP_153712742.1 aspartate carbamoyltransferase catalytic subunit -
  ACOQN4_RS05300 - 1034617..1035498 (+) 882 WP_420551088.1 dihydroorotase -
  ACOQN4_RS05305 - 1035463..1036302 (-) 840 WP_420551089.1 MBL fold metallo-hydrolase -
  ACOQN4_RS05310 pilU 1036375..1037493 (-) 1119 WP_153712739.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOQN4_RS05315 pilT 1037503..1038540 (-) 1038 WP_420551090.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOQN4_RS05320 - 1038572..1039270 (+) 699 WP_420551091.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACOQN4_RS05325 proC 1039267..1040067 (+) 801 WP_420551092.1 pyrroline-5-carboxylate reductase -
  ACOQN4_RS05330 - 1040091..1040672 (+) 582 WP_420551093.1 YggT family protein -
  ACOQN4_RS05335 metX 1040673..1041767 (+) 1095 WP_153712734.1 homoserine O-succinyltransferase MetX -
  ACOQN4_RS05340 metW 1041764..1042363 (+) 600 WP_420551094.1 methionine biosynthesis protein MetW -
  ACOQN4_RS05345 - 1042360..1042791 (+) 432 WP_153712732.1 DUF4426 domain-containing protein -
  ACOQN4_RS05350 rdgB 1042788..1043372 (+) 585 WP_420551095.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 37886.35 Da        Isoelectric Point: 6.5087

>NTDB_id=1171107 ACOQN4_RS05315 WP_420551090.1 1037503..1038540(-) (pilT) [MAG: Litorivicinus lipolyticus isolate cfb32c0c-0c5a-4fc9-8b22-2b72eeb7acf8]
MDITELLAFSAKQKASDLHLSAGLPPMIRVDGDVRRINVPELDHKAVHGLIYDIMNDKQRRDFEEKLETDFSFEVPGVAR
FRVNAFNQNRGAAAVFRTIPTEILSMEALGMGQVFRNLSDKPRGLVLVTGPTGSGKSTTLAAMVDYINETRNDHILTIED
PIEFVHPSKKSLVNQREVHRDTHGFSDALRSALREDPDVILVGEMRDLETISLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMVRSMLSESLQGVISQTLLKKNGGGRVAAHEIMMGTPAIRNLIREDKVAQMVSAIQTGSSMGMVTLDQSL
QTLVDKGVVSKEVARTKASRPDTFN

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1171107 ACOQN4_RS05315 WP_420551090.1 1037503..1038540(-) (pilT) [MAG: Litorivicinus lipolyticus isolate cfb32c0c-0c5a-4fc9-8b22-2b72eeb7acf8]
ATGGATATCACAGAGTTATTGGCCTTTAGCGCCAAACAGAAGGCCTCGGATTTACACCTGTCGGCGGGTTTGCCGCCGAT
GATTCGGGTGGACGGTGATGTCCGGCGTATCAATGTGCCTGAACTTGACCATAAAGCCGTGCATGGGCTGATTTACGACA
TCATGAACGACAAGCAACGACGTGACTTTGAAGAGAAGCTGGAAACAGACTTTTCGTTTGAAGTGCCCGGTGTGGCGCGT
TTTCGTGTCAACGCCTTTAATCAAAACCGTGGCGCGGCGGCGGTGTTCCGGACCATTCCAACCGAAATTTTGAGTATGGA
AGCGCTCGGCATGGGGCAGGTGTTCCGCAACTTGTCGGACAAACCGCGGGGTTTGGTGCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCGATTACATCAACGAGACCCGCAACGACCACATATTGACGATCGAAGAT
CCGATCGAGTTTGTGCACCCGTCGAAAAAATCCCTGGTCAACCAGCGTGAAGTCCACCGCGACACCCATGGGTTTAGCGA
CGCCCTGCGCAGCGCCCTGCGCGAAGACCCGGACGTGATTTTGGTCGGCGAGATGCGTGATTTAGAAACCATTAGCTTGG
CGCTGACGGCGGCTGAAACTGGCCACCTGGTGTTCGGCACGCTGCACACTACCAGTGCCGCCAAAACGATCGACCGTATT
ATCGACGTCTTCCCGGCGGCCGAGAAAGACATGGTCCGCTCGATGCTGTCCGAATCCTTGCAAGGTGTGATCAGCCAAAC
GCTATTGAAAAAGAACGGCGGCGGCCGGGTCGCGGCCCATGAAATCATGATGGGTACACCGGCGATTCGTAATCTGATAC
GCGAGGACAAGGTCGCGCAAATGGTCAGTGCGATCCAAACCGGCAGCTCGATGGGCATGGTTACCTTGGATCAGTCCCTA
CAGACCCTGGTCGATAAGGGGGTGGTCTCTAAAGAGGTTGCCCGGACCAAGGCATCCCGCCCCGATACGTTTAATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

83.43

99.71

0.832
  pilT Pseudomonas stutzeri DSM 10701

82.267

99.71

0.82
  pilT Acinetobacter baumannii strain A118

81.686

99.71

0.814
  pilT Acinetobacter nosocomialis M2

81.686

99.71

0.814
  pilT Acinetobacter baumannii D1279779

81.686

99.71

0.814
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Legionella pneumophila strain Lp02

72.674

99.71

0.725
  pilT Legionella pneumophila strain ERS1305867

72.674

99.71

0.725
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.03

97.681

0.684
  pilT Vibrio cholerae strain A1552

70.03

97.681

0.684
  pilT Neisseria meningitidis 8013

67.052

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.474

100

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Pseudomonas stutzeri DSM 10701

41.298

98.261

0.406
  pilU Vibrio cholerae strain A1552

41.791

97.101

0.406
  pilU Acinetobacter baylyi ADP1

39.017

100

0.391

Multiple sequence alignment