Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACOOFO_RS14860 Genome accession   NZ_OZ244162
Coordinates   3217448..3218491 (-) Length   347 a.a.
NCBI ID   WP_420427529.1    Uniprot ID   -
Organism   MAG: Algiphilus sp. isolate dd919ab0-ff71-4ff6-8152-ac5c3678e526     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3212448..3223491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOOFO_RS14840 - 3213863..3214567 (-) 705 WP_420427525.1 GNAT family N-acetyltransferase -
  ACOOFO_RS14845 - 3214798..3215172 (+) 375 WP_420427526.1 DUF6746 family protein -
  ACOOFO_RS14850 - 3215188..3216282 (-) 1095 WP_420427527.1 PilT/PilU family type 4a pilus ATPase -
  ACOOFO_RS14855 pilU 3216279..3217439 (-) 1161 WP_420427528.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOOFO_RS14860 pilT 3217448..3218491 (-) 1044 WP_420427529.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOOFO_RS14865 - 3218549..3219247 (+) 699 WP_420427530.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACOOFO_RS14870 rpiA 3219289..3219954 (+) 666 WP_420427531.1 ribose-5-phosphate isomerase RpiA -
  ACOOFO_RS14875 - 3219998..3220528 (+) 531 WP_420427532.1 DUF4399 domain-containing protein -
  ACOOFO_RS14880 - 3220536..3221303 (+) 768 WP_420427533.1 3-hydroxyacyl-CoA dehydrogenase -
  ACOOFO_RS14885 - 3221437..3222333 (+) 897 WP_420427534.1 HDOD domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38267.77 Da        Isoelectric Point: 6.6679

>NTDB_id=1171045 ACOOFO_RS14860 WP_420427529.1 3217448..3218491(-) (pilT) [MAG: Algiphilus sp. isolate dd919ab0-ff71-4ff6-8152-ac5c3678e526]
MDITQLLTFAVKQKASDLHLSAGVEPMIRVDGDVKRINLPPLEHDQVHGMIYDIMTDKQRKDYDEHFETDFSFEVQGLAR
FRVNAFNQARGAGAVFRTIPSTILTLEDLNAPEVFKKIIDVPRGIVLVTGPTGSGKSTTLAAMIDYINKTEYGHILTIED
PIEFVHQSNKCLINQREVHRDTLGFNEALRSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRAMLSESLRAVISQSLLKKKGGGRVAAHEIMIGNAAIRNLIRENKIAQMYSSIQTGGKEGMQTLDQNL
KKLVDRGIVSNSEARAYANSKADFAGG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1171045 ACOOFO_RS14860 WP_420427529.1 3217448..3218491(-) (pilT) [MAG: Algiphilus sp. isolate dd919ab0-ff71-4ff6-8152-ac5c3678e526]
ATGGATATCACGCAGTTGCTGACCTTCGCCGTCAAGCAGAAGGCGTCCGACCTGCACCTCTCGGCAGGTGTCGAGCCGAT
GATCCGGGTCGACGGAGACGTCAAGCGCATCAACCTGCCGCCGCTGGAGCACGACCAGGTGCACGGCATGATCTACGACA
TCATGACCGACAAGCAGCGCAAGGATTACGACGAGCATTTCGAGACCGATTTCTCCTTCGAGGTGCAGGGGCTTGCCCGC
TTCCGTGTCAATGCCTTCAATCAGGCACGCGGCGCCGGCGCCGTGTTCCGCACGATTCCGTCGACCATCCTGACCCTGGA
GGACCTCAACGCGCCCGAAGTGTTCAAGAAGATCATTGACGTGCCGCGCGGGATTGTGTTGGTGACCGGGCCCACCGGTT
CCGGTAAGTCGACCACACTGGCTGCGATGATCGACTACATCAACAAGACCGAATACGGGCACATTCTCACCATCGAAGAC
CCCATCGAGTTCGTACACCAGTCCAACAAGTGCCTGATCAATCAGCGTGAGGTGCACCGCGATACGCTGGGTTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAGGATCCGGACGTGGTGCTCGTCGGCGAGTTGCGCGACCTGGAGACCATCCGTCTGG
CACTGACGGCGGCCGAGACCGGGCACCTCGTCTTCGGCACCTTGCACACCAGTTCCGCGGCCAAGACCATTGACCGTATC
GTCGACGTCTTTCCGGCGGCGGAGAAGGATATGGTGCGCGCCATGCTGTCCGAGTCGCTGCGTGCGGTGATTTCACAGTC
GCTGCTCAAGAAGAAGGGCGGGGGTCGTGTGGCTGCGCACGAAATCATGATTGGCAATGCCGCCATCCGGAACCTGATCC
GCGAGAACAAGATCGCGCAGATGTATTCCTCGATTCAGACCGGCGGCAAGGAGGGCATGCAGACCCTCGACCAGAACCTC
AAGAAGCTGGTGGATCGTGGCATCGTGAGCAACAGCGAGGCGCGCGCCTACGCCAATTCCAAAGCCGATTTCGCGGGCGG
ATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.907

99.135

0.772
  pilT Acinetobacter nosocomialis M2

77.907

99.135

0.772
  pilT Acinetobacter baumannii D1279779

77.907

99.135

0.772
  pilT Acinetobacter baumannii strain A118

77.907

99.135

0.772
  pilT Pseudomonas stutzeri DSM 10701

77.616

99.135

0.769
  pilT Acinetobacter baylyi ADP1

76.453

99.135

0.758
  pilT Legionella pneumophila strain ERS1305867

72.965

99.135

0.723
  pilT Legionella pneumophila strain Lp02

72.965

99.135

0.723
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.345

96.83

0.671
  pilT Vibrio cholerae strain A1552

69.345

96.83

0.671
  pilT Neisseria meningitidis 8013

67.052

99.712

0.669
  pilT Neisseria gonorrhoeae MS11

66.763

99.712

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

99.712

0.499
  pilU Acinetobacter baylyi ADP1

41.52

98.559

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.87

99.424

0.406
  pilU Vibrio cholerae strain A1552

39.71

99.424

0.395

Multiple sequence alignment