Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACOOFO_RS14855 Genome accession   NZ_OZ244162
Coordinates   3216279..3217439 (-) Length   386 a.a.
NCBI ID   WP_420427528.1    Uniprot ID   -
Organism   MAG: Algiphilus sp. isolate dd919ab0-ff71-4ff6-8152-ac5c3678e526     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3211279..3222439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOOFO_RS14840 - 3213863..3214567 (-) 705 WP_420427525.1 GNAT family N-acetyltransferase -
  ACOOFO_RS14845 - 3214798..3215172 (+) 375 WP_420427526.1 DUF6746 family protein -
  ACOOFO_RS14850 - 3215188..3216282 (-) 1095 WP_420427527.1 PilT/PilU family type 4a pilus ATPase -
  ACOOFO_RS14855 pilU 3216279..3217439 (-) 1161 WP_420427528.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOOFO_RS14860 pilT 3217448..3218491 (-) 1044 WP_420427529.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOOFO_RS14865 - 3218549..3219247 (+) 699 WP_420427530.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACOOFO_RS14870 rpiA 3219289..3219954 (+) 666 WP_420427531.1 ribose-5-phosphate isomerase RpiA -
  ACOOFO_RS14875 - 3219998..3220528 (+) 531 WP_420427532.1 DUF4399 domain-containing protein -
  ACOOFO_RS14880 - 3220536..3221303 (+) 768 WP_420427533.1 3-hydroxyacyl-CoA dehydrogenase -
  ACOOFO_RS14885 - 3221437..3222333 (+) 897 WP_420427534.1 HDOD domain-containing protein -

Sequence

Protein


Download         Length: 386 a.a.        Molecular weight: 43718.82 Da        Isoelectric Point: 6.2509

>NTDB_id=1171044 ACOOFO_RS14855 WP_420427528.1 3216279..3217439(-) (pilU) [MAG: Algiphilus sp. isolate dd919ab0-ff71-4ff6-8152-ac5c3678e526]
MDREAAVRLVTQLLRMVKERGASDLFFTAGFPPAMKIDGELRPVMDRPLNAQQAALVVRSIMNDRQLRDFEATNECNFAI
SPQDIGRFRVNAFVQQNNTGAVLRPITTEIPTFEQLELPDTLRSVCMAKHGMVLMVGATGSGKSTSLAAMLGHRNGNSFG
HIVTIEDPIEFVHPHRQCVITQREVGVDTQTWDNALKNTLRQAPDVILIGEIRTRETMEYAINFAETGHLVLSTLHANSS
NQALDRIINFFPEDKREQVLMDLSLNLRAIVAQRLVRRENGGRKAAVEIMLNSPLVSDLIFKGEVAGIKETMTRSREQGM
QTFDQHLFELFEAGDISYEEALRNADSQNELRLRIKLESKRASRDLYNDPDVQKMSMHEDTEQRRW

Nucleotide


Download         Length: 1161 bp        

>NTDB_id=1171044 ACOOFO_RS14855 WP_420427528.1 3216279..3217439(-) (pilU) [MAG: Algiphilus sp. isolate dd919ab0-ff71-4ff6-8152-ac5c3678e526]
ATGGATCGAGAAGCCGCTGTTCGACTGGTCACCCAGCTGCTGCGCATGGTCAAGGAGCGCGGTGCCTCGGATCTGTTCTT
CACGGCCGGCTTCCCGCCCGCGATGAAAATCGACGGGGAGCTGCGGCCGGTGATGGACAGGCCGCTGAATGCCCAGCAGG
CGGCCCTCGTGGTGCGCAGCATCATGAACGACCGCCAGCTGCGCGACTTCGAAGCCACCAACGAGTGCAACTTCGCCATC
TCACCCCAGGACATCGGACGATTCCGCGTCAATGCCTTTGTTCAGCAGAACAATACTGGCGCGGTGCTGCGGCCGATCAC
CACCGAGATTCCGACCTTCGAACAGCTGGAGCTGCCCGACACACTGCGCTCGGTGTGCATGGCCAAGCACGGCATGGTGT
TGATGGTGGGGGCCACGGGCTCCGGCAAGTCGACCTCACTGGCGGCCATGCTGGGTCACCGCAATGGCAACTCCTTCGGT
CATATCGTCACGATCGAGGACCCCATCGAGTTCGTGCATCCGCACCGGCAGTGCGTGATCACGCAACGCGAGGTCGGCGT
GGATACGCAGACGTGGGATAACGCCCTCAAGAACACCCTGCGTCAGGCGCCGGATGTGATTCTGATCGGTGAGATCCGCA
CCCGCGAAACCATGGAATACGCCATCAACTTTGCGGAAACCGGCCACCTGGTGCTGTCCACGCTGCACGCCAATTCCTCG
AACCAGGCGCTGGACCGCATCATCAATTTCTTTCCCGAGGACAAGCGGGAACAGGTGCTGATGGACCTGTCGCTGAACCT
GCGCGCCATCGTCGCGCAGCGTCTGGTGCGGCGCGAGAATGGCGGCCGCAAGGCAGCGGTGGAGATCATGCTGAATTCAC
CGCTGGTCTCGGATCTGATCTTCAAGGGCGAGGTCGCCGGCATCAAGGAGACCATGACGCGCTCGCGCGAGCAGGGCATG
CAGACCTTTGACCAGCATCTCTTCGAACTCTTCGAGGCGGGCGACATCAGCTACGAGGAGGCGCTGCGCAATGCCGATTC
GCAGAATGAATTGCGCCTGCGCATCAAGCTCGAATCCAAGCGCGCCAGCCGTGATCTGTACAACGACCCGGATGTGCAGA
AGATGTCGATGCACGAGGACACCGAGCAGAGGCGCTGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.713

97.409

0.562
  pilU Acinetobacter baylyi ADP1

55.028

92.746

0.51
  pilU Vibrio cholerae strain A1552

53.501

92.487

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.606

88.86

0.396
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.899

89.378

0.383
  pilT Vibrio cholerae strain A1552

42.899

89.378

0.383
  pilT Acinetobacter baumannii strain A118

43.402

88.342

0.383
  pilT Acinetobacter nosocomialis M2

43.402

88.342

0.383
  pilT Acinetobacter baumannii D1279779

43.402

88.342

0.383
  pilT Acinetobacter baylyi ADP1

42.604

87.565

0.373
  pilT Pseudomonas aeruginosa PAK

42.262

87.047

0.368
  pilT Pseudomonas stutzeri DSM 10701

41.369

87.047

0.36
  pilT Legionella pneumophila strain ERS1305867

41.867

86.01

0.36
  pilT Legionella pneumophila strain Lp02

41.867

86.01

0.36

Multiple sequence alignment