Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACOFKU_RS13055 Genome accession   NZ_OZ241361
Coordinates   2830794..2831828 (+) Length   344 a.a.
NCBI ID   WP_419812586.1    Uniprot ID   -
Organism   MAG: Bacterioplanoides sp. isolate a8483998-2d78-4028-8a03-01e39642ae96     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2825794..2836828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOFKU_RS13035 - 2826250..2828238 (+) 1989 WP_419812582.1 DNA topoisomerase III -
  ACOFKU_RS13040 - 2828372..2828965 (-) 594 WP_419812583.1 YggT family protein -
  ACOFKU_RS13045 proC 2829039..2829857 (-) 819 WP_419812584.1 pyrroline-5-carboxylate reductase -
  ACOFKU_RS13050 - 2829943..2830629 (-) 687 WP_419812585.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACOFKU_RS13055 pilT 2830794..2831828 (+) 1035 WP_419812586.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOFKU_RS13060 pilU 2831882..2832997 (+) 1116 WP_419812587.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOFKU_RS13065 - 2833159..2834382 (+) 1224 WP_419812588.1 acyl-CoA dehydrogenase family protein -
  ACOFKU_RS13070 - 2834382..2835236 (+) 855 WP_419812589.1 SDR family oxidoreductase -
  ACOFKU_RS13075 - 2835233..2836327 (+) 1095 WP_419812590.1 phosphotransferase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37883.28 Da        Isoelectric Point: 6.4348

>NTDB_id=1170829 ACOFKU_RS13055 WP_419812586.1 2830794..2831828(+) (pilT) [MAG: Bacterioplanoides sp. isolate a8483998-2d78-4028-8a03-01e39642ae96]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPSLGHKEVHGLVYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMDQLGMGQVFKDLADTHKGIVLVTGPTGSGKSTTLAAMLDYINESKYYHILTVED
PIEFVHESKKSLINQREVHRDTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHVVFGTLHTSSAAKTIDRI
VDVFPSEEQAMVRSMLSESLQGVISQTLLKKSGGGRVAAHEIMVGTPAIRNLIREDKVAQMYSAIQTGGAYGMTTMDQSL
QTLVQKGLISRDTAREKAKVPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1170829 ACOFKU_RS13055 WP_419812586.1 2830794..2831828(+) (pilT) [MAG: Bacterioplanoides sp. isolate a8483998-2d78-4028-8a03-01e39642ae96]
ATGGATATTACCGAACTGTTGGCGTTCAGCGCGAAGCAAGGCGCGTCCGACTTACACCTGTCGGCAGGCCTGCCCCCTAT
GATTCGTGTTGACGGCGATGTGCGTCGTATTAATCTGCCGTCGCTGGGCCATAAAGAAGTACACGGTCTGGTATATGACA
TCATGAACGATAAGCAGCGTAAGGATTTTGAAGAATTCCTTGAGACTGACTTCTCCTTCGAAGTGCCGGGCGTGGCGCGT
TTCCGGGTAAACGCCTTTAACCATAACCGTGGTGCTGGCGCGGTATTCCGGACCATTCCTTCCAAAGTACTGACCATGGA
CCAGCTAGGCATGGGTCAGGTGTTTAAAGACCTGGCAGATACCCATAAAGGTATTGTGCTGGTAACTGGACCAACCGGTT
CGGGTAAGTCGACCACGCTGGCGGCGATGCTGGATTACATTAACGAAAGTAAGTATTACCACATCCTGACGGTAGAAGAT
CCGATCGAATTTGTGCACGAATCGAAGAAATCCCTGATTAACCAACGGGAAGTACACCGTGACACCCTGGGCTTTAACGA
AGCGCTGCGTTCTGCCCTGCGGGAAGACCCAGATGTGATTCTGGTGGGCGAGATGCGAGACCTGGAAACCATCCGTCTGG
CGTTAACTGCAGCCGAAACCGGTCACGTGGTGTTTGGTACGTTGCATACCAGCTCGGCAGCTAAAACCATTGACCGGATT
GTTGACGTGTTCCCGTCCGAAGAACAAGCCATGGTACGTTCAATGCTGTCTGAGTCTCTGCAGGGCGTGATCTCTCAGAC
ACTGCTGAAGAAAAGTGGCGGTGGTCGTGTGGCGGCTCACGAAATCATGGTCGGTACTCCGGCGATTCGTAACCTGATTC
GTGAAGATAAAGTGGCGCAGATGTATTCTGCTATCCAGACCGGTGGTGCCTATGGCATGACCACCATGGACCAGTCATTA
CAGACACTGGTTCAGAAGGGTTTGATTAGCCGTGATACCGCTCGTGAAAAAGCCAAAGTGCCGGATAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

86.337

100

0.863
  pilT Pseudomonas stutzeri DSM 10701

84.884

100

0.849
  pilT Acinetobacter nosocomialis M2

83.14

100

0.831
  pilT Acinetobacter baumannii D1279779

83.14

100

0.831
  pilT Acinetobacter baumannii strain A118

83.14

100

0.831
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

73.077

98.256

0.718
  pilT Legionella pneumophila strain ERS1305867

73.077

98.256

0.718
  pilT Neisseria meningitidis 8013

67.941

98.837

0.672
  pilT Neisseria gonorrhoeae MS11

67.647

98.837

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.062

97.965

0.657
  pilT Vibrio cholerae strain A1552

67.062

97.965

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Vibrio cholerae strain A1552

39.42

100

0.395
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387
  pilU Pseudomonas stutzeri DSM 10701

39.104

97.384

0.381

Multiple sequence alignment