Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACOFKU_RS13060 Genome accession   NZ_OZ241361
Coordinates   2831882..2832997 (+) Length   371 a.a.
NCBI ID   WP_419812587.1    Uniprot ID   -
Organism   MAG: Bacterioplanoides sp. isolate a8483998-2d78-4028-8a03-01e39642ae96     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2826882..2837997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOFKU_RS13040 - 2828372..2828965 (-) 594 WP_419812583.1 YggT family protein -
  ACOFKU_RS13045 proC 2829039..2829857 (-) 819 WP_419812584.1 pyrroline-5-carboxylate reductase -
  ACOFKU_RS13050 - 2829943..2830629 (-) 687 WP_419812585.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACOFKU_RS13055 pilT 2830794..2831828 (+) 1035 WP_419812586.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOFKU_RS13060 pilU 2831882..2832997 (+) 1116 WP_419812587.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOFKU_RS13065 - 2833159..2834382 (+) 1224 WP_419812588.1 acyl-CoA dehydrogenase family protein -
  ACOFKU_RS13070 - 2834382..2835236 (+) 855 WP_419812589.1 SDR family oxidoreductase -
  ACOFKU_RS13075 - 2835233..2836327 (+) 1095 WP_419812590.1 phosphotransferase family protein -
  ACOFKU_RS13080 - 2836329..2837018 (+) 690 WP_419812591.1 histidine phosphatase family protein -
  ACOFKU_RS13085 - 2837051..2837821 (+) 771 WP_419812592.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41149.30 Da        Isoelectric Point: 6.2877

>NTDB_id=1170830 ACOFKU_RS13060 WP_419812587.1 2831882..2832997(+) (pilU) [MAG: Bacterioplanoides sp. isolate a8483998-2d78-4028-8a03-01e39642ae96]
MAMDKFLRLMVEKGASDLFITAGVPPSMKVHGSIMPLTKNPLGAEQTRDIVLGLMNGKQQDEFEEKKELNFAINASGIGR
FRASAFYQRNVAGMVLRRIETRIPSLDQLGLPDIIKDLSMSKRGLILFVGATGAGKSTSLAAMIGYRNRNSKGHILSIED
PIEFIHQHESCIVTQREVGLDTETFEIGLKNCLRQAPDVIMIGEVRSADTMEHAITFAETGHLCLATLHSNNANQALERV
IHFFPTERHSQIWMDLSLNLKAIIAQQLIPTPDGKGRRAVIEVLINTPLASDLIRKGEVHELKPLMRKSNEVGMQTYDQA
LYALYEQGEITYEDAIAHADSPNDLRLMIKLGSETDADSLDSATSKLRLQD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=1170830 ACOFKU_RS13060 WP_419812587.1 2831882..2832997(+) (pilU) [MAG: Bacterioplanoides sp. isolate a8483998-2d78-4028-8a03-01e39642ae96]
ATGGCTATGGATAAGTTTTTGCGTCTGATGGTGGAAAAAGGCGCCTCCGATTTATTTATCACCGCCGGTGTTCCGCCGTC
GATGAAGGTGCATGGCAGCATCATGCCACTGACGAAAAATCCGCTGGGTGCCGAACAAACCCGCGATATTGTGCTGGGCC
TGATGAACGGCAAGCAACAGGATGAGTTTGAAGAGAAAAAAGAACTCAACTTTGCCATTAATGCCAGTGGTATTGGTCGT
TTTCGGGCGTCGGCGTTTTATCAGCGTAACGTCGCCGGTATGGTGTTGCGGCGGATTGAGACCCGGATTCCGAGTCTGGA
TCAGCTTGGTCTGCCCGACATTATCAAAGATTTGTCGATGTCCAAGCGTGGCCTGATTTTATTTGTGGGTGCCACCGGTG
CCGGTAAATCAACCTCGCTGGCAGCGATGATTGGTTATCGTAACCGCAATTCCAAAGGCCATATTCTCAGCATTGAAGAC
CCGATTGAATTTATCCATCAGCACGAAAGCTGCATTGTTACCCAGCGGGAAGTGGGGTTGGATACTGAGACGTTTGAGAT
TGGTCTGAAGAACTGTCTGCGTCAGGCTCCGGATGTCATCATGATTGGTGAGGTACGGTCGGCGGATACCATGGAGCACG
CCATCACCTTTGCTGAAACCGGCCACTTATGTCTGGCGACGCTGCACTCCAACAACGCCAACCAGGCCCTGGAACGAGTG
ATTCACTTTTTCCCAACTGAGCGCCATAGCCAGATCTGGATGGATTTATCCCTCAACTTAAAAGCCATTATTGCCCAGCA
ATTAATTCCAACACCGGATGGCAAAGGTCGTCGTGCGGTGATTGAAGTATTAATTAATACACCGCTGGCATCGGATTTAA
TTCGTAAAGGCGAAGTACACGAATTAAAACCGCTGATGCGTAAATCTAACGAAGTGGGCATGCAGACCTACGACCAGGCG
CTGTACGCGTTATACGAGCAGGGTGAAATCACTTACGAAGATGCCATTGCTCATGCGGACTCGCCAAACGATCTGCGCCT
GATGATTAAACTGGGCTCAGAAACCGATGCTGATTCGCTGGATAGTGCAACTTCCAAGCTGAGACTGCAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

73.784

99.73

0.736
  pilU Acinetobacter baylyi ADP1

61.905

96.226

0.596
  pilU Vibrio cholerae strain A1552

56

94.34

0.528
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.068

91.375

0.394
  pilT Pseudomonas aeruginosa PAK

39.71

92.992

0.369
  pilT Acinetobacter nosocomialis M2

39.766

92.183

0.367
  pilT Acinetobacter baumannii D1279779

39.766

92.183

0.367
  pilT Acinetobacter baumannii strain A118

39.766

92.183

0.367
  pilT Legionella pneumophila strain Lp02

40

90.296

0.361
  pilT Legionella pneumophila strain ERS1305867

40

90.296

0.361

Multiple sequence alignment