Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACOFKU_RS09240 Genome accession   NZ_OZ241361
Coordinates   2002299..2003399 (-) Length   366 a.a.
NCBI ID   WP_419811906.1    Uniprot ID   -
Organism   MAG: Bacterioplanoides sp. isolate a8483998-2d78-4028-8a03-01e39642ae96     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1997299..2008399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOFKU_RS09225 - 1997923..1998477 (-) 555 WP_419811904.1 PilN domain-containing protein -
  ACOFKU_RS09230 pilM 1998477..1999538 (-) 1062 WP_419813659.1 pilus assembly protein PilM Machinery gene
  ACOFKU_RS09235 - 1999749..2002274 (+) 2526 WP_419811905.1 penicillin-binding protein 1A -
  ACOFKU_RS09240 pilU 2002299..2003399 (-) 1101 WP_419811906.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOFKU_RS09245 - 2003531..2004949 (-) 1419 WP_419811907.1 FAD-binding oxidoreductase -
  ACOFKU_RS09250 serA 2005241..2006470 (+) 1230 WP_419811908.1 phosphoglycerate dehydrogenase -
  ACOFKU_RS09255 - 2006634..2007470 (+) 837 WP_419811909.1 flagellin -
  ACOFKU_RS09260 hisF 2007521..2008309 (-) 789 WP_419811910.1 imidazole glycerol phosphate synthase subunit HisF -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40572.59 Da        Isoelectric Point: 9.5632

>NTDB_id=1170822 ACOFKU_RS09240 WP_419811906.1 2002299..2003399(-) (pilU) [MAG: Bacterioplanoides sp. isolate a8483998-2d78-4028-8a03-01e39642ae96]
MTITKLLQVLAKHGGSDLYLSTGAAPSAKFQGNLKALSKEPLKPGQIEAMVNDIIDDEQRQEFEKELELNIAISLPVGRF
RMNIFRQRHEVSLVARNIAAEIPKFEALGLPEILKTVIMAKRGLLLFVGATGSGKSTSLASLIDHRNSHSAGHIITIEDP
VEYVHSHKKSIVNQREVGTDTKSFHRALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRIM
NFFPEDRRQQLRQDLGLNLRAIVSQRLIPTTDSKRVAAFEVLLGNKTIEELILKGDIAGIKEIMEKSENLGMQTFDTSLF
RLYQAGRISLEEALKNADSENNLRLRIKLGKKAGSSQSASEGMSIT

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=1170822 ACOFKU_RS09240 WP_419811906.1 2002299..2003399(-) (pilU) [MAG: Bacterioplanoides sp. isolate a8483998-2d78-4028-8a03-01e39642ae96]
ATGACCATTACTAAACTGCTGCAGGTATTGGCTAAACACGGTGGCTCAGACCTGTATTTGAGTACCGGAGCCGCTCCCAG
TGCGAAGTTCCAGGGGAATCTGAAAGCTCTGAGTAAGGAGCCTTTAAAGCCGGGCCAGATTGAAGCGATGGTCAACGATA
TTATTGACGATGAACAACGTCAGGAATTTGAAAAAGAGCTGGAGTTGAACATTGCGATCAGCTTGCCTGTTGGTCGTTTC
CGGATGAATATTTTTCGTCAGCGCCATGAGGTTTCATTAGTGGCCCGAAATATTGCGGCTGAGATCCCTAAGTTCGAAGC
GCTGGGGCTGCCGGAAATCTTGAAAACAGTCATTATGGCAAAGCGGGGTCTGTTGTTATTTGTTGGGGCGACCGGCTCGG
GTAAATCAACATCGCTGGCGTCACTGATTGATCATCGTAACAGCCATTCGGCAGGTCACATTATTACGATTGAAGACCCG
GTTGAGTACGTTCATTCCCATAAAAAGTCGATTGTGAATCAACGAGAAGTGGGAACAGATACTAAGTCTTTCCATCGCGC
TCTGAAAAATACCCTGCGCCAGGCACCGGATGTCATTCTGATTGGCGAAATCCGTGATCGTGAAACCATGGAGCATGCCT
TGGCGTTTTCTGAAACCGGGCACCTGGCCATTTCGACGTTACATGCAAACAATGCTAACCAGGCATTAGATCGCATTATG
AACTTCTTTCCCGAAGATCGTCGTCAGCAATTACGGCAGGATCTTGGCCTGAATCTGCGGGCTATTGTATCTCAGCGTCT
TATTCCAACAACCGACAGCAAACGAGTGGCGGCTTTTGAAGTGTTGCTTGGCAATAAAACCATTGAGGAGTTAATACTGA
AAGGCGATATTGCGGGTATCAAAGAGATCATGGAAAAGTCTGAGAATCTGGGTATGCAAACCTTTGACACATCTCTGTTC
AGACTTTACCAGGCGGGTCGGATTTCATTAGAAGAGGCGTTAAAAAATGCCGATTCTGAAAATAACCTTCGTTTGCGTAT
TAAGCTGGGTAAAAAAGCAGGCTCTTCTCAGTCTGCTTCTGAAGGGATGAGCATTACGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

53.678

100

0.538
  pilU Pseudomonas stutzeri DSM 10701

53.117

100

0.536
  pilU Vibrio cholerae strain A1552

48.424

95.355

0.462
  pilT Pseudomonas aeruginosa PAK

41.86

93.989

0.393
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.433

92.077

0.391
  pilT Pseudomonas stutzeri DSM 10701

40.407

93.989

0.38
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.541

90.984

0.369
  pilT Vibrio cholerae strain A1552

40.541

90.984

0.369
  pilT Legionella pneumophila strain ERS1305867

40.909

90.164

0.369
  pilT Legionella pneumophila strain Lp02

40.909

90.164

0.369
  pilT Neisseria meningitidis 8013

39.13

94.262

0.369
  pilT Neisseria gonorrhoeae MS11

39.13

94.262

0.369
  pilT Acinetobacter baumannii strain A118

40.12

91.257

0.366
  pilT Acinetobacter baumannii D1279779

40.12

91.257

0.366
  pilT Acinetobacter nosocomialis M2

40.12

91.257

0.366
  pilT Acinetobacter baylyi ADP1

39.118

92.896

0.363

Multiple sequence alignment