Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACOFKU_RS09230 Genome accession   NZ_OZ241361
Coordinates   1998477..1999538 (-) Length   353 a.a.
NCBI ID   WP_419813659.1    Uniprot ID   -
Organism   MAG: Bacterioplanoides sp. isolate a8483998-2d78-4028-8a03-01e39642ae96     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1993477..2004538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOFKU_RS09205 aroK 1994114..1994623 (-) 510 WP_419811900.1 shikimate kinase AroK -
  ACOFKU_RS09210 pilQ 1994627..1996735 (-) 2109 WP_419811901.1 type IV pilus secretin PilQ Machinery gene
  ACOFKU_RS09215 - 1996738..1997277 (-) 540 WP_419811902.1 pilus assembly protein PilP -
  ACOFKU_RS09220 - 1997279..1997923 (-) 645 WP_419811903.1 type 4a pilus biogenesis protein PilO -
  ACOFKU_RS09225 - 1997923..1998477 (-) 555 WP_419811904.1 PilN domain-containing protein -
  ACOFKU_RS09230 pilM 1998477..1999538 (-) 1062 WP_419813659.1 pilus assembly protein PilM Machinery gene
  ACOFKU_RS09235 - 1999749..2002274 (+) 2526 WP_419811905.1 penicillin-binding protein 1A -
  ACOFKU_RS09240 pilU 2002299..2003399 (-) 1101 WP_419811906.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38543.43 Da        Isoelectric Point: 4.1202

>NTDB_id=1170821 ACOFKU_RS09230 WP_419813659.1 1998477..1999538(-) (pilM) [MAG: Bacterioplanoides sp. isolate a8483998-2d78-4028-8a03-01e39642ae96]
MASLFNKKSKALIGVDISSTSVKVLELSHNNGRYQVEAYAAEPLPPNAIVEQAISNDEAVGEAIRKALGRSRTSAKRAAI
AVAGSAVITKTVQMSSKLNDDEMDLQIRAEADQYIPYPLEEVALDWEVQGESEQAADMVDVLLAACRSETVERRKDAVEY
ADLEAGIVDVEAFCTERAFDLLQSQLDGDDIETVAVLDIGATMTTLSVLHEGKSIYTREQLFGGRQLTEDIMRRYGLSEE
EATRSKLDGDLPDDYESEVLDPFRKAVVQQVSRSLQFFYSSSQFNDVDFIILAGGTSAIPMLAECVEEELGVATLIANPF
MNMTLSQKVNASLLANDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=1170821 ACOFKU_RS09230 WP_419813659.1 1998477..1999538(-) (pilM) [MAG: Bacterioplanoides sp. isolate a8483998-2d78-4028-8a03-01e39642ae96]
CTGGCCAGCCTGTTTAATAAAAAGAGCAAAGCGCTGATTGGTGTGGATATCAGTTCTACATCGGTCAAGGTGCTGGAGCT
TAGTCATAATAATGGACGCTATCAGGTTGAAGCTTACGCGGCAGAGCCTTTACCACCGAATGCTATTGTTGAACAGGCTA
TCAGCAATGATGAGGCTGTTGGTGAGGCAATTCGAAAAGCGCTTGGTCGCTCGCGCACTTCAGCTAAACGTGCGGCAATT
GCCGTTGCTGGTTCTGCGGTTATCACTAAAACCGTTCAGATGAGTTCCAAACTTAATGACGATGAAATGGATTTGCAGAT
CCGTGCCGAGGCCGATCAGTATATTCCCTACCCGTTAGAAGAAGTTGCTCTGGATTGGGAAGTTCAGGGGGAATCTGAAC
AAGCAGCCGATATGGTTGATGTTCTGTTAGCAGCTTGCCGTTCAGAAACGGTTGAACGCCGTAAAGATGCTGTTGAGTAT
GCCGACCTTGAAGCGGGTATTGTTGATGTTGAAGCTTTCTGTACAGAGCGTGCTTTCGATTTACTGCAAAGCCAACTAGA
CGGTGATGATATCGAGACAGTTGCTGTGCTTGATATTGGTGCAACCATGACCACACTGAGTGTGCTGCATGAAGGCAAAT
CAATTTACACCCGTGAACAGTTATTTGGTGGTCGTCAGCTAACGGAAGATATTATGCGCCGTTATGGGTTGTCTGAAGAA
GAGGCGACACGCTCCAAACTGGACGGGGATTTACCGGATGACTACGAAAGTGAAGTACTGGATCCATTCCGCAAAGCGGT
TGTGCAGCAAGTAAGTCGCTCTCTGCAATTTTTCTATTCTTCCAGCCAGTTCAACGATGTCGACTTTATTATCCTTGCTG
GCGGCACTTCAGCGATTCCGATGTTGGCAGAGTGTGTTGAGGAAGAGCTGGGCGTTGCCACTCTGATCGCCAACCCTTTT
ATGAATATGACCTTGTCGCAAAAAGTAAATGCCAGTCTGTTAGCGAACGATGCCCCTTCACTGATGATTGCCTGCGGTCT
GGCGATGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.958

100

0.55
  comM Acinetobacter nosocomialis M2

54.958

100

0.55
  comM Acinetobacter baylyi ADP1

49.858

100

0.499
  pilM Legionella pneumophila strain ERS1305867

43.38

100

0.436

Multiple sequence alignment